Purification and identification of the growth promoting substance from corn fiber and its promotion mechanisms on useful mushroom productions

玉米纤维促生长物质的纯化、鉴定及其对有用菌增产的促进机制

• 批准号: 15580151
• 负责人: TERASHITA Takao
• 金额: $ 1.98万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580151/
• 关键词: Mushroom; Mushroom Cultivation; Corn fiber; Corn fiber Waste Product; Growth Promoting Substance for mushroom

Corn fiber (CNF) is an abundant by-product of the wet corn milling process in the production of corn starch. We have shown that the hot water soluble fraction (HWSF) from CNF has a promoting effect on the mycelial growth of various edible mushrooms including mycorrhizal mushroom such as Tricholoma matsutake and so on. Moreover, fruit-body yield increased at 1.2-1.6 times by using CNF, and the cultivation period was dramatically shortened compared to that of the control without CNF. To reveal the promoting mechanisms, the effect of CNF-HWSF on the stimulation of extracellular enzymes was examined. As a result, the production of carbohydrases such as amylase, CMCase and xylanase were markedly enhanced by the addition of low molecular weight fractions (less than M.W.500) prepared from CNF-HWSF. The enzymatic stimulations and enhancement of mycelial growth appeared during the about same time on the culture period. Furthermore, fraction less than the M.W.500 was separated by gel filtration into 5 fractions, and the effect of each fraction was investigated. The promoting effects were indicated even on a fraction D has no sugars and amino acids in CNF-HWSF, From these results, the promoting effect by CNF-HWSF seems to be a 2 step reactions. The first step could be achieve by rich nutrients such as mono- and ologo-saccharides and free amino acids from CNF-HWSF. The second step considered that the marked promoting effect was caused by the stimulation of extracellular enzymes. Now, we are in the improvement stage for practical application of CNF as a growth substrate using sawdust-based cultivation. Further attempts will be made to investigate the isolation and the characterization of promoting substances in CNF-HWSF to clarify the promotion mechanisms on edible mushrooms.

玉米纤维（CNF）是玉米淀粉生产过程中玉米湿磨过程中产生的大量副产物。结果表明，CNF的热水可溶物（HWSF）对包括松茸等菌根菌在内的多种食用菌菌丝生长均有促进作用，子实体产量比对照提高1.2-1.6倍，培养周期明显缩短。为了揭示促进机制，检查CNF-HWSF对胞外酶的刺激的效果。结果表明，添加CNF-HWSF制备的低分子量组分（小于M.W.500）可显著提高淀粉酶、羧甲基纤维素酶和木聚糖酶等糖酶的产量。在培养过程中，酶促反应和菌丝生长的促进作用几乎同时出现。此外，通过凝胶过滤将小于M.W.500的级分分离成5个级分，并研究每个级分的效果。CNF-HWSF中不含糖和氨基酸的组分D也显示出促进作用。从这些结果来看，CNF-HWSF的促进作用似乎是两步反应。第一步可以通过CNF-HWSF中丰富的营养物质如单-和低聚糖和游离氨基酸来实现。第二步认为，显著的促进作用是由胞外酶的刺激引起的。现在，我们正处于将CNF作为生长基质进行锯末栽培的实际应用的改进阶段。本研究将进一步探讨CNF-HWSF中促进物质的分离与表征，以阐明CNF-HWSF对食用菌的促进机制。

项目成果

Promotive effect of the hot water-soluble fraction from corn fiber on vegetative Mycelial growth in edible mushrooms

玉米纤维热水可溶部分对食用菌营养菌丝生长的促进作用

• 发表时间: 2003
• 期刊: J.Wood Science 49・5
• 作者: Yasue Arai;Yasue ARAI;荒井 康恵;Yasue Arai
• 通讯作者: Yasue Arai

コーンファイバー添加培地で栽培された食用きのこ類の機能性評価

玉米纤维培养基中食用菌的功能评价

• 期刊: 日本きのこ学会誌 (発表予定)
• 作者: Yasue Arai;Yasue ARAI;荒井 康恵;Yasue Arai;Yasue ARAI;Yasue ARAI;Yasue Arai;Yasue Arai;寺下 隆夫;白坂 憲章
• 通讯作者: 白坂 憲章

食用きのこ菌床栽培への廃棄物コーンファイバー(CNF)の利用

利用废弃玉米纤维（CNF）进行食用菌床栽培

• 发表时间: 2003
• 期刊: 日本応用きのこ学会誌 11・1
• 作者: Yasue Arai;Yasue ARAI;荒井 康恵
• 通讯作者: 荒井 康恵

The stimulation of extracellular carbohydrases of edible mushrooms By the hot water soluble fraction from corn fiber

玉米纤维热水可溶部分对食用菌胞外糖酶的刺激

• 期刊: Mycoscience (In press)
• 作者: Yasue Arai;Yasue ARAI;荒井 康恵;Yasue Arai;Yasue ARAI;Yasue ARAI;Yasue Arai;Yasue Arai
• 通讯作者: Yasue Arai

The sutimulation of extracellular carbohydrases of edible mushrooms by hot water soluble fraction from corn fiber

玉米纤维热水溶组分对食用菌胞外糖酶的刺激

• 期刊: Mycoscience 46(4)(in press)
• 作者: Yasue Arai;Yasue ARAI;荒井 康恵;Yasue Arai;Yasue ARAI;Yasue ARAI;Yasue Arai;Yasue Arai;寺下 隆夫;白坂 憲章;Yasue ARAI
• 通讯作者: Yasue ARAI