权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Exploitation of effective uses of Rubisco as highly functional food resources

Rubisco作为高功能食品资源的有效利用

基本信息

批准号：
12793007
负责人：
YOSHIKAWA Masaaki
金额：
$ 19.58万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for University and Society Collaboration
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Ribulose bisphosphate carboxylase/oxygenase (Rubisco) which catalizes carbon dioxide fixation in photosynthesis is the most abundant protein on the earth since it occupys 1/3 to 1/2 of leaf proteins. Exceptionally as a plant protein, Rubisco has an ideal amino acid composition from nutritional point of view. We screened for bioactive peptides which might contribute to prevent lifestyle-related diseases in enzymatic digests of Rubisco. Rubiscolin (Tyr Pro-Leu- Asp Leu- Phe), which was isolated as a δ opioid peptide from a pepsin digest of spinach Rubisco, improved learning performance after oral administration at a dose of 100mg/kg in mice. It also exhibited anxiolytic activity. Four inhibitory peptides for angiotensin-converting enzyme, MRWRD, MRW, LRIPVA and IAYKPAG, were isolated from pepsin pancreatin digest of spinach Rubisco. Among them, MRWRD MRW and IAYKPAG showed hypotensive activity after oral administration in spontaneously hypertensive rats (SHR). Crude protein fraction of which Rubisco content was higher than 50% was prepared from spinach and digested with pepsin and pancreatin. ACE-inhibitory activities of the digests from the crude leaf protein were higher than those from purified rubisco. Especially, pepsin digest of the crude leaf protein exhibited potent antihypertensive activity suggesting that ingestion of leaf protein is effective in preventing hypertension. Essentially the same bioactive peptides could be released from Rubisco of other plant species. Rubisco is expected as an ideal protein resouce which could be produced under small environmental load.Rubisco is an important enzyme protein in lowering atmospheric carbon dioxide. This means that Rubisco is a very important proteins not only as a food resources but also in improving environment.

二磷酸核酮糖羧化酶/加氧酶（Rubisco）是光合作用中催化二氧化碳固定的酶，是地球上最丰富的蛋白质，占叶片蛋白质的1/3 ~ 1/2。Rubisco作为一种特殊的植物蛋白，从营养学的角度来看具有理想的氨基酸组成。我们筛选了可能有助于预防Rubisco酶促代谢物中生活方式相关疾病的生物活性肽。Rubiscolin（Tyr Pro-Leu-AspLeu-Phe）是从菠菜Rubisco的胃蛋白酶消化物中分离得到的一种δ阿片肽，经口给药100 mg/kg后，小鼠的学习成绩得到改善。它还表现出抗焦虑活性。从菠菜Rubisco的胃蛋白酶胰酶消化物中分离得到四种血管紧张素转换酶抑制肽MRWRD、MRW、LRIPVA和IAYKPAG。其中MRWRD、MRW和IAYKPAG对自发性高血压大鼠（SHR）口服后有降压作用。以菠菜为原料，经胃蛋白酶和胰酶消化，得到Rubisco含量高于50%的粗蛋白组分。从粗叶蛋白中提取的酶的ACE抑制活性高于从纯化的Rubisco中提取的酶。特别是，粗叶蛋白的胃蛋白酶消化物显示出有效的抗高血压活性，表明摄入叶蛋白可有效预防高血压。从其他植物Rubisco中也能释放出基本相同的活性肽。Rubisco是一种理想的蛋白质资源，可以在低环境负荷下生产，是降低大气CO2排放的重要酶蛋白。这意味着Rubisco不仅是一种重要的食品资源，而且在改善环境方面也是一种非常重要的蛋白质。