Regulation of artery, immune and nervous systems by albutensin derived from serum albumin

血清白蛋白衍生的阿布紧张素对动脉、免疫和神经系统的调节

• 批准号: 07660162
• 负责人: YOSHIKAWA Masaaki
• 金额: $ 1.66万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660162/
• 关键词: serum albumin; albutensin; complement; tumour necrosis factor; phagocytosis; bombesin; food intake; neuropeptide Y; 補体C3a; 補体C5a; 免疫促進

1. Bovine albutensin A (Ala-Leu-Lys-Ala-Trp-Ser-Val-Ala-Arg) was isolated from the tryptic digest of bovine serum albumin as an ileum-contracting peptide. Human and porcine counterparts (Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg and Ala-Phe-Lys-Ala-Trp-Ser-Leu-Ala-Arg) were also ileum-contracting peptides. These peptides were agonists for complement C3a and C5a. They stimulated phagocytosis and induction of TNF.2. Bovine albutensin C (Arg-His-Pro-Glu-Tyr-Ala-Val-Ser-Val-Leu-Leu-Arg) was also isolated from the tryptic digest of bovine serum albumin as an ileum-contracting peptide. Human and porcine counterparts did not show the same activity. Albutensin C was also an agonist for C3a receptor and stimulated phagocytosis.3. Human albutensin A showed affinity for bombesin receptor (IC_<50>=133muM). This peptide stimulated food intake at a dose of 10nmol (icv.) in fasted mice. However, it suppressed food intake at a dose of 30 nmol. Human albutensin A seems to be a partial agonist for bombesin receptor because bombesin suppress food intake. On the other hand, synthetic human des-Arg-albutensin A-NH_2 also showed affinity for bombesin receptor and supressed food intake at 100 nmol. It didn't stimulate food intake and seems to be a bombesin agonist. Bovine and porcine albutensin A did had no effect on food intake because their affinities for bombesin receotor were low.4. A synthetic fragment peptide of bovine serum albumin, Tyr-Leu-Ser-Leu-Ile-Leu-Asn-Arg showed affinity for neuropeptide Y_2 receptor. This peptide was named albutensin B.Albutensin might be an antagonist for neuropeptide Y because it suppressed food intake in fasted mice after icv.

1.牛白蛋白紧张素A（Ala-Leu-Lys-Ala-Trp-Ser-Val-Ala-Arg）是从牛血清白蛋白的胰蛋白酶消化物中分离得到的回肠收缩肽。人和猪对应物（Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg和Ala-Phe-Lys-Ala-Trp-Ser-Leu-Ala-Arg）也是回肠收缩肽。这些肽是补体C3 a和C5 a的激动剂。它们刺激吞噬作用和TNF的诱导。牛白蛋白紧张素C（Arg-His-Pro-Glu-Tyr-Ala-Val-Ser-Val-Leu-Leu-Arg）也从牛血清白蛋白的胰蛋白酶消化物中分离作为回肠收缩肽。人类和猪的对应物没有显示出相同的活性。白蛋白紧张素C也是C3 a受体的激动剂，并刺激吞噬.人白蛋白紧张素A对蛙皮素受体具有亲和力（IC_<50>（133）μ M）。该肽在10 nmol（icv.）在禁食的小鼠中。然而，它在30 nmol剂量下抑制食物摄入。人白蛋白紧张素A可能是蛙皮素受体的部分激动剂，因为蛙皮素抑制摄食。另一方面，合成的人des-Arg-albutensin A-NH_2也显示出与蛙皮素受体的亲和力，在100 nmol时抑制摄食。它不会刺激食物摄入，似乎是一种铃蟾肽激动剂。牛和猪白蛋白紧张素A对摄食量没有影响，因为它们对蛙皮素受体的亲和力较低.人工合成的牛血清白蛋白片段肽Tyr-Leu-Ser-Leu-Ile-Leu-Asn-Arg与神经肽Y_2受体有亲和力。白蛋白紧张素B可能是神经肽Y的拮抗剂，因为白蛋白紧张素抑制icv后禁食小鼠的摄食。

项目成果

吉川正明: "食品タンパク質からの生理活性ペプチドの生産とプロテアーゼの選択" 酪農科学・食品の研究. 45(3). A51-A60 (1996)

Masaaki Yoshikawa：“从食品蛋白质生产生物活性肽和蛋白酶的选择”，乳制品科学和食品研究 45(3) (1996)。

吉川正明: "食品タンパク質からの生理活性ペプチドの生産とプロテアーゼの選択" 酪農科学・食品の研究. 45(3). A51-A60 (1997)

Masaaki Yoshikawa：“从食品蛋白质生产生物活性肽和蛋白酶的选择”，乳制品科学与食品研究 45(3) (1997)。

H.Fujita et al.: "vasorelaxation by casomokinin L,a derivative of beta-casomorphin and casoxin D,is mediated by NK_1 receptor." Peptides.17 (4). 635-639 (1997)

H.Fujita 等人：“casomokinin L（β-casomorphin 和 casoxin D 的衍生物）的血管舒张作用是由 NK_1 受体介导的。”

H.Fujita: "Vasorelaxation by casomokinin L,a derivative of β-casomorphin and casoxin D,is mediated by NK_1 receptor." Peptides. 17(4). 635-639 (1996)

H. Fujita：“casomokinin L（β-casomorphin 和 casoxin D 的衍生物）的血管舒张作用是由 NK_1 受体介导的。” 635-639 (1996)。

M.Yoshikawa: "Selection of proteases for the production of bioactive peptides derivved from food" Jap.J.Dairy Food Sci.45 (3). A51-A60 (1997)

M.Yoshikawa：“用于生产源自食品的生物活性肽的蛋白酶的选择”Jap.J.Dairy Food Sci.45 (3)。