Epidemiological research of the endocrine disruptors in wild primates as a human model in Thailand

泰国野生灵长类动物模型内分泌干扰物的流行病学研究

• 批准号: 12836004
• 负责人: SUZUKI Juri
• 金额: $ 2.3万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12836004/
• 关键词: Primate; Endcrine disruptors; IGF-1; Sexual steroids; 内分泌撹乱物質

Capturing work of wild long-tailed macaques and rhesus macaques were carried out in the northeastern part of Thailand. Blood samples were collected from those macaque groups. As survey of the phthalate esters in blood, plasma di(n-butyl)phthalate and di(2-ethylhexyl)phthalate were measured. The result indicated that examined animals were polluted by those phthalate esters. This shows that the environmental pollution by the phthalate esters may have been spread over in Thailand. IGF-1 and sexual steroids (testosterone in male, estrogen and progesterone in female) were measured. Plasma level of IGF-1 in long-tailed macaques was slightly lower than that in Japanese macaques. Pregnant individuals exhibited higher levels of the hormone. Those values were within the range of IGF-1 concentrations in pregnant wild Japanese macaques. Age-related changes in testosterone showed a temporal rise in infant period and permanent rise after 5 years. These are similar to those in captive animals. However, the second (permanent) rise in wild animals was one or two years later than that in captive ones. Age-related changes in female sexual steroids were similar to those in captive animals. Histological specimens were made from gonadal organs of captive macaques by biopsy in Thailand. Histological analysis of those specimens showed no any abnormalities. This result indicated that it may be difficult to prove the influence of endocrine disrupters by this approach.An epidemiological proof of the endocrine disruption by the phthalate ester to wild macaques in Thailand was detected hi this research. Those wild macaques were evaluated as a useful epidemiological monitor for humans. However, the necessity for more precisely research cannot be undervalued.

在泰国东北部进行了野生长尾猕猴和恒河猴的捕获工作。从这些猕猴组中采集血样。作为血液中邻苯二甲酸酯的调查，测定了血浆邻苯二甲酸二（正丁基）酯和邻苯二甲酸二（2-乙基己基）酯。结果表明，受检动物均受到邻苯二甲酸酯类物质的污染。这表明，邻苯二甲酸酯的环境污染可能已经扩散到泰国。测定IGF-1和性类固醇激素（男性睾酮，女性雌激素和孕激素）。长尾猕猴血浆IGF-1水平略低于日本猕猴。怀孕的人表现出更高的激素水平。这些值在妊娠野生日本猕猴的IGF-1浓度范围内。睾丸激素的变化在婴儿期呈暂时性上升，5年后呈永久性上升。这些与圈养动物的情况相似。然而，野生动物的第二次（永久）上升比圈养动物晚一两年。雌性性类固醇激素的变化与圈养动物相似。组织学标本由泰国圈养猕猴的性腺器官活检制成。这些标本的组织学分析显示无任何异常。这一结果表明，通过这种方法可能很难证明内分泌干扰物的影响。本研究检测到了邻苯二甲酸酯对泰国野生猕猴内分泌干扰的流行病学证据。这些野生猕猴被评价为人类有用的流行病学监测者。然而，不能低估进行更精确研究的必要性。

项目成果

Shimizu, Y., Suzuki, J., Terao, K., Ishida, T.: "In vitro aging of macaque adherent cells : similar pattern of cellular aging between human and macaque"Mechanisms of Ageing and Development. 124. 237-244 (2003)

Shimizu, Y.、Suzuki, J.、Terao, K.、Ishida, T.：“猕猴贴壁细胞的体外衰老：人类和猕猴之间细胞衰老的相似模式”衰老和发育的机制。

Suzuki, J., Kato, A., Maeda, N., Hashimoto, C., Uchikoshi, M., Mizutanim T., Doke, C., Matsuzawa, T.: "Plasma insulin-like growth factor-I(IGF-1), testosterone and morphological changes in the growth of captive agile gibbons (Hylobates agilis) from birth

Suzuki, J.、Kato, A.、Maeda, N.、Hashimoto, C.、Uchikoshi, M.、Mizutanim T.、Doke, C.、Matsuzawa, T.：“血浆胰岛素样生长因子-I（IGF）

Suzuki, J.: "Age changes in plasma IGF-1 concentration in free-ranging Japanese macaques (Macaca fuscata)"Journal of Medical Primatology. 30. 174-178 (2001)

Suzuki, J.：“自由放养的日本猕猴（Macaca fuscata）血浆 IGF-1 浓度的年龄变化”医学灵长类动物学杂志。

Suzuki, J.: "Plasma insulin-like growth factor-I(IGF-1), testosterone and morphological changes in the growth of captive agile gibbons (Hylobates agilis) from birth to adolescence"Primates. (in press). (2003)

Suzuki, J.：“血浆胰岛素样生长因子-I (IGF-1)、睾酮和圈养敏捷长臂猿 (Hylobates agilis) 从出生到青春期生长的形态变化”灵长类动物。

Suzuki, J.: "Baseline and stress levels of cortisol in conscious and unrestrained Japanese macaques (Macaca fuscata)"Journal of Medical Primatology. 32. 340-344 (2002)

Suzuki, J.：“有意识且不受约束的日本猕猴（Macaca fuscata）皮质醇的基线和压力水平”《医学灵长类动物学杂志》。