Function of the plant gene induced by oxidative stress on the reduciton of lipid hydroperoxide

氧化应激诱导的植物基因对脂质过氧化氢还原的作用

基本信息

  • 批准号:
    12660295
  • 负责人:
  • 金额:
    $ 1.98万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

The gene that includes the promoter region of the phospholipid hydroperoxide glutathione peroxidase (PHGPX)-like protein was cloned from Arabidopsis thaliana. The gene was subcloned into a plasmid, pBI101, resulting the promoter gene was fused with the β-glucuronidase (GUS) gene. The fused gene was transferred to the root of the pea by the particle bombardment system. Transient expression of the GUS was shown in the roots cultured by the MS medium containing 0, 50, and 100mM NaCl. This result suggests that the PHGPX-like gene is not induced by oxidative/salt stress.A cDNA encoding barley PHGPX-like protein was expressed in Escherichia coli cells to produce an extra protein. The PHGPX was not detected in the cell-free extract prepared from the clone cells when tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide were used as a substrate. The clone cells were assayed for their ability to grow in the medium containing NaCl. The clone cells were much more tolerant to NaCl stress than the host cells, suggesting that the PHGPX-like protein would be a good candidate component of anti-salt/oxidative enzyme in plants.
从拟南芥中克隆了含磷脂过氧化氢谷胱甘肽过氧化物酶(PHGPX)类蛋白启动子区域的基因。将该基因亚克隆到pBI101中,得到启动子基因与β-葡萄糖苷酸酶(GUS)基因的融合。通过基因枪法将融合基因转入豌豆的根中。在含有0、50和100 mM氯化钠的MS培养基中,GUS基因在根中瞬时表达。这一结果表明PHGPX-like基因不是由氧化/盐胁迫诱导的。大麦PHGPX-like蛋白的编码基因在大肠杆菌中表达,以产生额外的蛋白。以叔丁基氢过氧化氢、过氧化异丙苯和过氧化氢为底物时,从克隆细胞制备的无细胞提取物中未检测到PHGPX。检测克隆细胞在含有氯化钠的培养液中的生长能力。克隆细胞比寄主细胞对盐胁迫的耐受性更强,这表明PHGPX类蛋白可能是植物抗盐/氧化酶的一个很好的候选成分。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Manabu Sugimoto: "Structure and function of a phospholipid hydroperoxide glutathione peroxidase-like protein from barley"Journal of Molecular Catalysis B : Enzymatic. (in press). (2003)
Manabu Sugimoto:“来自大麦的磷脂氢过氧化物谷胱甘肽过氧化物酶样蛋白的结构和功能”分子催化杂志 B:酶学。
  • DOI:
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    0
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  • 通讯作者:
Manabu Sugimoto, Kazuyoshi Takeda: "Structure and function of a phospholipid hydroxide glutathione peroxidase-like protein from barley"Journal of Molecular Catalysis B : Enzymatic. (in press). (2003)
Manabu Sugimoto、Kazuyoshi Takeda:“来自大麦的磷脂氢氧化物谷胱甘肽过氧化物酶样蛋白的结构和功能”分子催化杂志 B:酶学。
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    0
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SUGIMOTO Manabu其他文献

SUGIMOTO Manabu的其他文献

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{{ truncateString('SUGIMOTO Manabu', 18)}}的其他基金

Electronic-Structure Simulation Study on Molecular Structure andProperties of Graphitic Carbon Nitride
石墨碳氮化物分子结构与性能的电子结构模拟研究
  • 批准号:
    22550019
  • 财政年份:
    2010
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Electronic Function Simulation of Surface Coordination Space
表面协调空间的电子函数仿真
  • 批准号:
    16074213
  • 财政年份:
    2004
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Development of recycling biomass for recycling systems of organic waste
开发用于有机废物回收系统的回收生物质
  • 批准号:
    15580297
  • 财政年份:
    2003
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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