LOCALIZATION OF THE NOVEL RNA BINDING PROTEIN SLA1 AND ITS ROLE IN CELL DIFFERENTIATION IN FISSION YEAST

新型RNA结合蛋白SLA1的定位及其在裂殖酵母细胞分化中的作用

• 批准号: 12660301
• 负责人: KAWAMUKAI Makoto
• 金额: $ 2.3万
• 依托单位: SHIMANE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660301/
• 关键词: RAS; cAMP; Signal transduction; fission yeast; Sla1; Sla1

Sla1, a homolog of human La protein in fission yeast, has been known to be an RNA binding protein that has an RRM (RNA-Recognition Motif) and the La motif which are highly conserved among La protein families. In this study, we present the evidence, for the first time among the La homologs, that a truncated form of Sla1 is important for regulation of sexual development in Schizosaccharomyces pombe. We found that Sla1 truncated in the COOH-terminus (Sla1DC) induced ectopic sporulation in ras1 strain and other several sporulation deficient strains such as disruptants of the components of the MAPK-cascade, the SAPK-cascade and the cAMP-cascade. The full-length of Sla1 located in nucleus, but sporulation inducible Sla1DC located in cytoplasm. Consistent with this result, Sla1 has a basic NLS (Nuclear Localization Signal)-like motif in its COOH terminus. Further deletion analysis of Sla1 suggested that for sporulation inducible effect a small region that includes only a portion of RRM, but neither a whole RRM nor the La motif was required. In addition, Sla1DC induced ectopic haploid meiosis, like pat1-114 or mei2-SATA mutant do. Sla1DC induced sporulation in a mei3 disruptant but not in a mei2 disruptant. These results strongly suggested that Sla1DC acts at the upstream of Mei2 and at the downstream of Mei3.

SLA 1是裂殖酵母中人La蛋白的同源物，已知是具有在La蛋白家族中高度保守的RRM（RNA识别基序）和La基序的RNA结合蛋白。在这项研究中，我们提出的证据，第一次在La同系物，即SLA 1的截短形式是重要的粟酒裂殖酵母的性发育的调节。我们发现，在COOH-末端截短的Sla 1（Sla 1DC）诱导的异位孢子形成在ras 1菌株和其他几个孢子形成缺陷株，如破坏的MAPK-级联，SAPK-级联和cAMP-级联的组件。Sla 1全长定位于细胞核，而可诱导孢子形成的Sla 1DC定位于细胞质。与该结果一致，Sla 1在其COOH末端具有碱性NLS（核定位信号）样基序。进一步的删除分析SLA 1的孢子形成诱导的影响，一个小的区域，包括RRM的一部分，但既不需要一个完整的RRM也不La基序。此外，Sla 1DC诱导异位单倍体减数分裂，就像pat 1 -114或mei 2-SATA突变体一样。sla 1DC诱导产孢mei 3破坏，但不是在mei 2破坏。这些结果有力地表明Sla 1DC作用于Mei 2的上游和Mei 3的下游。

项目成果

Guo - Lei Zhou, Takaharu Yamamoto, Fumiyo Ozoe, Daisuke Yano, Katsunori Tanaka, Hideyuki Matsuda and Makoto Kawamukai: "Identification of a 14-3-3 protein from Lentinus edodes that interacts with CAP (adenylyl cyclase - associated protein), and conservati

郭 - Lei Zhou、Takaharu Yamamoto、Fumiyo Ozoe、Daisuke Yano、Katsunori Tanaka、Hideyuki Matsuda 和 Makoto Kawamukai：“香菇中与 CAP（腺苷酸环化酶相关蛋白）相互作用的 14-3-3 蛋白的鉴定，以及保守性

Kumiko Shimono: "Functional expression of chitinase and chitosanase, and their effects on morhphologies in the yeast S. pombe"Biosci.Biotech.Biochem.. (印刷中).

Kumiko Shimono：“几丁质酶和壳聚糖酶的功能表达及其对酵母裂殖酵母形态的影响”Biosci.Biotech.Biochem..（出版中）。

Kumiko Shimono: "Functional expression of chitinase and chitosanase, and their effects on morhphologies in the yeast"Biosci. Biotech. Biochem.. (印刷中).

Kumiko Shimono：“几丁质酶和壳聚糖酶的功能表达及其对酵母形态的影响”Biosci Biochem..（出版中）。

Guo-Lei Zhou: "Identification of a 14-3-3 protein from Lentinus edodes that interacts with CAP, and conservation of this interaction in fission yeast"Biosci.Biotech.Biochem.. 64. 149-159 (2000)

周国雷：“香菇中与 CAP 相互作用的 14-3-3 蛋白的鉴定，以及裂殖酵母中这种相互作用的保存”Biosci.Biotech.Biochem.. 64. 149-159 (2000)

Naonori Uchida: "Phenotypes of fission yeast defective in ubiquinone production due to disruption of the gene"J. Bacteriol. 182. 6933-6939 (2000)

Naonori Uchida：“由于基因破坏导致裂殖酵母在泛醌生产中出现缺陷的表型”J.