Correlated Light and Electron Microscopic Study of the Plasmalemmal Undercoats

质膜底毛的相关光和电子显微镜研究

• 批准号: 12670004
• 负责人: MURAKAMI Tohru
• 金额: $ 1.79万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670004/
• 关键词: plasmalemmal undercoats; cytoskeletons; cell adhesion; development; molecular architectures; corrated microscopy; confocal microscopy; electron microscopy; 形質膜; 共焦点レーザー顕微鏡; 電子顕微鏡; 平滑筋細胞; プレクチン; 神経周膜

Cytoskeletons are among the key components of prokaryotic cells playing such rolls as a cellular mechanical support as well as cytokinesis, cell adhesion, signal transmission, cell differentiation and development. Many of their functions are based on the mechanical and structural associations with cell adhesion structures and plasmalemmal undercoat. We studied such associations in muscle cells and perineurial cells by electron microscopy correlated with confocal microscopy and biochemical techniques. Additionally we investigated the induction of cell adhesion by rostral determinant gene Otx in developing vertebrate embryos by correlated molecular biology and microscopy. We also identified a protocadherin as a morphoregulator of somitogenesis.

细胞骨架是原核细胞的重要组成部分，在细胞分裂、细胞粘附、信号传递、细胞分化和发育等方面起着重要的作用。它们的许多功能是基于与细胞粘附结构和质膜底涂层的机械和结构关联。我们研究了这种协会在肌肉细胞和神经束膜细胞的电子显微镜与共聚焦显微镜和生化技术。此外，我们还通过相关的分子生物学和显微镜技术研究了喙决定基因Otx对脊椎动物胚胎细胞粘附的诱导作用。我们还确定了原钙粘蛋白作为一个形态调节器的体节发生。

项目成果

Abou Salem EA, Ishikawa H.: "Early morphological changes in the rat soleus muscle induced by tenotomy and denervation"J Electron Microsc. 50. 275-282 (2001)

Abou Salem EA、Ishikawa H.：“腱切断术和去神经支配引起的大鼠比目鱼肌的早期形态变化”J Electron Microsc。

Tanaka H, Hijikata T, Murakami T, Fujimaki N, Ishikawa H: "Localization of Plectin and Other Related Proteins along the Sarcolemma in Smooth Muscle Cells of Rat Colon"Cell Struct Funct. 26. 61-70 (2001)

Tanaka H、Hijikata T、Murakami T、Fujimaki N、Ishikawa H：“大鼠结肠平滑肌细胞中肌膜上的凝集素和其他相关蛋白的定位”细胞结构功能。

Abou Salem EA, Fujimaki N, Ishikawa H, Tashiro T, Komiya Y: "Morphological changes and recovery process in the tenotomized soleus muscles of the rat"Arch Histol Cytol. 64. 127-37 (2001)

Abou Salem EA、Fujimaki N、Ishikawa H、Tashiro T、Komiya Y：“大鼠腱切断比目鱼肌的形态变化和恢复过程”Arch Histol Cytol。

Abou Salem EA, Ishikawa H: "Early morphological changes in the rat soleus muscle induced by tenotomy and denervation"J Electron Microsc. 50. 275-282 (2001)

Abou Salem EA、Ishikawa H：“腱切断术和去神经支配引起的大鼠比目鱼肌的早期形态变化”J Electron Microsc。

Hijikata T: "Molecular organization of intermediate filament networks in skeletal muscle fibers (English Translation of Japanese title)"Seitai-no-Kagaku. 52. 287-293 (2001)

土方 T：“骨骼肌纤维中中间丝网络的分子组织（日文标题的英文翻译）”Seitai-no-Kagaku。