Functional Analyses of Protocadherin 10 in Embryonic Development of Central Nervous System and Paraxial Mesoderm

研究人员和合作者研究了原钙粘附素-10(Pcdh1O)和其他钙粘附素在斑马鱼发育中的生物学功能。原位杂交显示，pcdh1Ob表达于旁中胚层(PAM)和发育中的体节，在松果体、间脑和耳囊周围表达。在体节成熟过程中，PAM和体节的表达减少。Pcdh1Ob的显性-负性和反义Morpholino干扰了PAM的收敛和体节分割。比较研究表明，pcdh1Ob和pcdh8在PAM和近轴中的表达存在差异。我们认为Pcdh1Ob参与了PAM和体节段的汇聚，Pcdh8和Pcdh10b的差异黏附在体节和近轴细胞的形态发生机制中发挥了作用。我们克隆了…更像是人类K-钙粘素的斑马鱼同源物。整装ISH结果显示，除中枢和外周神经系统外，CDH6在前肾管和肾单位原基中也有表达。注射针对cdh6基因的mOS(Cdh6mos)的胚胎在30hpf时出现头部小、体轴弯曲、卵黄伸短、尾巴弯曲等发育缺陷，42hpf时出现胸腔水肿。用原肾标记WTL和pax2.1对注射了cdh6mos的胚胎进行原位杂交，观察原肾管前区和肾单位原基发育受阻的情况。组织学检查显示，前肾畸形为融合异常的肾小球原基、融合或异常弯曲的前肾小管原基和细小的前肾管。这些结果表明，cdh6在斑马鱼胚胎原肾的发育过程中起着关键作用。表达于中枢神经系统、咽弓和肠原基。研究结果目前正在以科学报告的形式进行汇编。较少

The investigator and collaborators studied biological functions of Protocadherin-lO (Pcdh1O) and other cadherins in zebrafish development. In situ hybridization (ISH) showed that pcdh1Ob was expressed in the paraxial mesoderm (PAM) and developing somites, and in the pineal body, the diencephalon and the vicinity of otocysts. Expression in PAM and somites decreased during somite maturation. A dominant-negative form of Pcdh1Ob and anti-sense Morpholino of pcdh1Ob disturbed convergence of PAM and somite segmentation. Comparative studies showed that differential expressions of pcdh1Ob and pcdh8 in PAM and the adaxial. We propose that Pcdh1Ob is involved in the convergence of PAM and somite segmentation, and that differential adhesion of Pcdh8 and Pcdh10b plays a role in the morphogenic machinery of somites and adaxial cells.We performed functional analyses of cadherin-6 (cdh6) in zebrafish nephrogenesis using antisense morpholino oligonucleotide (MO) inhibition combined with ISH. We cloned … More a zebrafish homolog of human K-cadherin. Whole-mount ISH showed that cdh6 is expressed in the pronephric ducts and nephron primordium in addition to the central and peripheral nervous systems. Embryos injected with MOs directed against cdh6 (cdh6MOs) showed developmental defects including small head, body axis curvature, short yolk extension, and short bent tail by 30 hpf, and edema appeared in the thorax by 42 hpf. Embryos injected with cdh6MOs were subjected to in situ hybridization with pronephric markers, wtl and pax2.1, to examine disturbed development of the anterior region of the pronephric ducts and the nephron primordia. Histological studies showed malformation of the pronephros as abnormally fused glomerulus primordia, fused or abnormally bent pronephric tubule anlage, and thin pronephric ducts. These results suggested that cdh6 plays pivotal roles in development of the pronephros in zebrafish embryos.We also studied zebrafish Protocadherin-18. It was expressed central nervous system, pharyngeal arches and gut primodia. The results are currently to be compiled as a scientific report. Less