The study on the morphogenesis of peroxisomes employing a targeting signal and its receptors

靶向信号及其受体过氧化物酶体形态发生的研究

• 批准号: 12670012
• 负责人: USUDA Nobuteru
• 金额: $ 1.79万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670012/
• 关键词: peroxisomes; signal peptides; targeting signals; morphogenesis

Peroxisomes have been stained by expressing a fusion protein of green fluorescent protein with a targeting signal in its C-terminus (GFP-SKL), introduced to cultured cells by trasfection procedure and to mice by making a trasgenic mouse. The object of the study is to elucidate the mechanism of morphogenesis, with these cells and transgenic mouse as materials. The present study was attempted to show the appearance of peroxisomes during cell division, embryonic development and by the administration of peroxisome proliferators. A tansgenic mouse expressing. GFP-SKL has been developed and biochemical and morphological analyses are on the way. Morphological analyses in cultured cells expressing GFP-SKL have been analyzed and on the way of publication. Related subjects on peroxisomes have been published.

通过表达在其C-末端具有靶向信号的绿色荧光蛋白的融合蛋白（GFP-SKL）来染色过氧化物酶体，通过转染程序将其引入培养的细胞，并通过制备转基因小鼠引入小鼠。本研究的目的是以这些细胞和转基因小鼠为材料，阐明其形态发生的机制。本研究试图展示细胞分裂、胚胎发育过程中以及通过给予过氧化物酶体增殖剂时过氧化物酶体的出现。转基因小鼠表达。已经研制出绿色荧光蛋白----国家重点实验室，正在进行生物化学和形态学分析。已对表达GFP-SKL的培养细胞进行了形态学分析，并即将发表。过氧化物酶体的相关课题已经发表。

项目成果

Nakashiro KI他: "Role of peroxisomeproferatpvates receptor gamma and its ligands in non-neoplastic and neoplastic human urothelial"Am J Pathol. 159. 591-597 (2001)

Nakashiro KI 等：“过氧化物酶体增殖物受体 γ 及其配体在非肿瘤性和肿瘤性人类尿路上皮中的作用”Am J Pathol. 159. 591-597 (2001)

Hamano H他: "High serum IgG4 concentrations patients with sclerosing pancreatitis"N Engl J Med. 344. 732-738 (2001)

Hamano H 等人：“患有硬化性胰腺炎的高血清 IgG4 浓度患者”N Engl J Med 344. 732-738 (2001)

Hamano H, Kawa S, Horiuchi A, Unno H, Furuya N, Akamatsu T, Fukushima M, Nikaido T, Nakayama K, Usuda N, Kiyosawa K: "High serum IgG4 concentrations in patients with sclerosing pancreatitis"N Engl J Med. 344 (10). 732-8 (2001)

Hamano H、Kawa S、Horiuchi A、Unno H、Furuya N、Akamatsu T、Fukushima M、Nikaido T、Nakayama K、Usuda N、Kiyosawa K：“硬化性胰腺炎患者血清 IgG4 浓度较高”N Engl J Med。

Karasawa N.他: "Distribution of histamine-containing neurons in the …"Acta Histochem.Cytochem.. 34. 431-439 (2001)

Karasawa N. 等人：“含组胺神经元在……中的分布”Acta Histochem.Cytochem.. 34. 431-439 (2001)

Ito R.他: "Temperature-sensitive phenotype of Chinese hamster ovary …"Biochem Biophys.Res.Commun. 288. 321-327 (2001)

Ito R. 等人：“中国仓鼠卵巢的温度敏感表型……”Biochem Biophys.Res.Commun 288. 321-327 (2001)