Molecular biological studies of the effect of moxibustion on diabetes mellitus
艾灸治疗糖尿病作用的分子生物学研究
基本信息
- 批准号:18390210
- 负责人:
- 金额:$ 10.84万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Object of the study : Comprehensive studies on the gene expression in various organs of rats with diabetes mellitus type 2 and metabolic syndrome. The analysis was performed by DNA microarray to reveal the effect of moxibustion on diabetes mellitus, whose metabolic changes concerning its pathogenesis and treatment of the disease. Morphological and clinico-pathological analysis on the blood and tissues were also done.Methods : SHR(mc-cp)cp/cp rats and WKY rats were mainly employed. Gene expression had been planned to be analyzed using fluorescent differential display and real time PCR, however, DNA microarray was employed due to its higher reliability in quantitative analysis. Physiological experiments, conditioned place preference, microdialysis was added in order to know the timing for moxibustion treatment.Results : The secretion of a neurotransmitter, dopamine, was observed in the nucleus accumbens 30 min. after the moxibustion. Conditioned place preference was positive. Microarray analysis revealed gene expression changed in most number of genes in the olfactory bulb and epididymal white adipose tissue.Discussion : In contrast to a popular view on the effect of moxibustion, which is merely produced by the heat produced by the burning of moxa, its effect on the animal bodies were suggested to be provoked by the combination of heat and the aroma of moxa. Based on physiological and molecular biological results obtained in the present study, moxibustion results in the changes of gene expression in the brain. This may change the the behavior of individuals and the cure of chronic diseases.
研究目的:综合研究2型糖尿病合并代谢综合征大鼠各器官基因表达。通过DNA芯片分析艾灸对糖尿病的影响,揭示其代谢变化与糖尿病的发病机制和治疗有关。同时对血液和组织进行了形态学和临床病理分析。方法:以SHR(mc-cp)cp/cp大鼠和WKY大鼠为主。原计划采用荧光差示显示和实时PCR分析基因表达,但由于定量分析的可靠性更高,采用了DNA芯片。通过生理实验、条件偏好、微透析等方法了解艾灸治疗时机。结果:艾灸30 min后观察到伏隔核分泌神经递质多巴胺。条件位置偏好为正。微阵列分析显示,嗅球和附睾白色脂肪组织中大部分基因的表达发生了变化。讨论:与普遍认为艾灸的效果仅仅是由艾草燃烧产生的热量产生的观点相反,它对动物身体的影响被认为是由热量和艾草的香气结合引起的。根据本研究获得的生理和分子生物学结果,艾灸会导致大脑中基因表达的变化。这可能会改变个人的行为和慢性疾病的治疗。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Phospholylation of neuronal nitric oxide synthase at Ser847 in the nucleus intermediolateralis after spinal cord injury in mice.
小鼠脊髓损伤后中间外侧核神经元一氧化氮合酶 Ser847 的磷酸化。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Inukai T;Osuka K;Takagi T;Usuda N;Yoshida J;Takayasu M.;Sarkar J;Osuka K
- 通讯作者:Osuka K
「研究成果報告書概要(和文)」より
摘自《研究结果报告摘要(日文)》
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Kawauchi;et. al.;Nishimura et al.;Dezawa et al.;Yoshizawa et al.;星野 幹雄;星野 幹雄
- 通讯作者:星野 幹雄
Phosphorylation of neuronal nitric oxide synthase at Ser847 in the nucleus intermediolateralis after spinal cord injury in mice
- DOI:10.1016/j.neuroscience.2006.10.034
- 发表时间:2007-03-02
- 期刊:
- 影响因子:3.3
- 作者:Osuka, K.;Watanabe, Y.;Yoshida, J.
- 通讯作者:Yoshida, J.
Activation of c-jun in the rat basilar artery after subarachnoid hemorrhage.
蛛网膜下腔出血后大鼠基底动脉中 c-jun 的激活。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Inukai T;Osuka K;Takagi T;Usuda N;Yoshida J;Takayasu M.
- 通讯作者:Takayasu M.
Transcription coactivator PRIP, the peroxisome proliferator-activated receptor(PPAR)-interacting protein, is redundant for the function of nuclear receptors PPARα and CAR, the constitutive androstane receptor, in mouse liver.
转录辅激活因子 PRIP 是一种过氧化物酶体增殖物激活受体 (PPAR) 相互作用蛋白,对于小鼠肝脏中核受体 PPARα 和 CAR(组成型雄甾烷受体)的功能来说是多余的。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Inukai T;Osuka K;Takagi T;Usuda N;Yoshida J;Takayasu M.;Sarkar J
- 通讯作者:Sarkar J
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USUDA Nobuteru其他文献
USUDA Nobuteru的其他文献
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{{ truncateString('USUDA Nobuteru', 18)}}的其他基金
The basic investigation on the organ differences of fatty acid beta-oxidation system
脂肪酸β-氧化系统器官差异的基础研究
- 批准号:
25504016 - 财政年份:2013
- 资助金额:
$ 10.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Neuroscientific studies on the effect of moxibustion on chronic diseases
艾灸对慢性病影响的神经科学研究
- 批准号:
20390200 - 财政年份:2008
- 资助金额:
$ 10.84万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The study on the morphogenesis of peroxisomes employing a targeting signal and its receptors
靶向信号及其受体过氧化物酶体形态发生的研究
- 批准号:
12670012 - 财政年份:2000
- 资助金额:
$ 10.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The study on the morphogenesis of peroxisomes by the inhibition of the expression of transcription factors, PPARs.
通过抑制转录因子 PPAR 的表达来研究过氧化物酶体的形态发生。
- 批准号:
10670011 - 财政年份:1998
- 资助金额:
$ 10.84万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Immuno-electron microscopy on the morphological marker enzymes and proteins for subcompartments of cell organellae.
免疫电子显微镜观察细胞器亚区室的形态标记酶和蛋白质。
- 批准号:
05670005 - 财政年份:1993
- 资助金额:
$ 10.84万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
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全国针灸使用情况及用户满意度调查[J].
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Clinical Study on the Use of Traditional Medicine in Pregnancy and for Safe Delivery - Effectiveness and Safety of Moxibustion Therapy -
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