The proliferation and the differentiation of the fundic gland constituent cells by means of immunohistochemical and molecular biological methods

免疫组织化学和分子生物学方法观察胃底腺组成细胞的增殖和分化

• 批准号: 12670023
• 负责人: MURATA Fusayoshi
• 金额: $ 2.18万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670023/
• 关键词: Mucous neck cell; Intermediate cell; Chief cell; glycoconjugate; lectin; immunohistochemistry; Pepsinogen C; in situ hybridization; 高圧凍結; 凍結置換; H-K-ATPase; 壁細胞; in situ hybridization

Mucous neck cell is a seromucous cell and chief cell is a serous cell. Intermediate cell between mucous neck cell and chief cell is also discemible in the fundic gland. The granule of intermediate cell has a biphasic structure consist of serous and mucous portions. Using electron microscopical, glycoconjugate histochemical, immunohistochemical and in situ hybridization techniques, we have been studying the differentiation of various cell types in the fundic gland. PA-TCH-SP staining, lectin colloidal gold staining and HIK 1083 staining labeled mucous neck cell, intermediate cell in relation to its glycoconjugate concentration in the granule. In the intermediate cell, only mucous portion of this granule was labeled by these staining but chief cell granule was not labeled by these staining. Immunohistochemistry of pepsinogen C stained these three types of cells in crescent manner of labeling density. In protein level, it has been verified that the mucous neck is the precursor of the chief cell. Messenger RNA of pepsinogen C was also ascertained in mucous neck cell and chief cell. This means that our proposal has also been ascertained in the gene level

黏液颈细胞是浆液细胞，主细胞是浆液细胞。在基底腺中，粘液颈细胞和主细胞之间的中间细胞也不可见。中间细胞颗粒具有由浆液和黏液部分组成的双相结构。我们利用电镜、糖结合组织化学、免疫组织化学和原位杂交技术，研究了不同类型细胞在基底腺中的分化。PA-TCH-SP染色、凝集素胶体金染色和HIK 1083染色标记黏液颈细胞，中间细胞与其颗粒中糖缀合物的浓度有关。在中间细胞中，只有该颗粒的粘液部分被标记，而主细胞颗粒未被标记。胃蛋白酶原C免疫组化对三种细胞呈新月形标记密度。在蛋白水平上，已证实黏液颈是主细胞的前体。在黏液颈细胞和主细胞中也检测到胃蛋白酶原C的信使RNA。这意味着我们的建议在基因水平上也得到了证实

项目成果

Yang D-H. et al.: "Expression on N-acetylglucosamine residues in developing rat fundic gland cells"Histochemical J.. 32. 187-193 (2000)

杨D-H.

Hasui K. et al.: "Immunohistochemical analysis of the pathogenicity of helicobacter pylori infection : excess nitric oxide induced indirectly by Lewis X and Y is the cause of the pathogenicity of helicobacter pylori infestation in the stomach"Acta Histoch

Hasui K.等人：“幽门螺杆菌感染致病性的免疫组织化学分析：Lewis X和Y间接诱导的过量一氧化氮是胃内幽门螺杆菌感染致病性的原因”Acta Histoch

村田長芳: "ゴルジ装置"日本臨床電子顕微鏡学会編. 46-47 (2002)

Nagayoshi Murata：日本临床电子显微镜学会编辑的“高尔基体”46-47（2002）。

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Murata F：“高压冷冻：过去、现在、未来”Electron Microsc。

Murata F. et al.: "The Golgi apparatus, in Monograph"Gakusaikikaku. 46-47 (2002)

Murata F.等人：“高尔基体，专着”Gakusaikikaku。