权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Histochemical analysis of the digestive tract glycoconjugates

消化道糖复合物的组织化学分析

基本信息

批准号：
62570012
负责人：
MURATA Fusayoshi
金额：
$ 1.09万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

During past two years, the following four projects have been studied in my research group.(1) Using rat stomach and colon as model organ, the optimal staining conditions of labeled lectins have been established. In our study, postembedding staining method was used in combination to the colloidal gold labeled lectins and hydrophilic resin. Among many research groups, our group belongs the one which uses many kinds of lectins in the experiment.(2) Using above-mentioned staining conditions, we stained the various cell types of the rat hundic gland. We observed the decrease of the labeling intensity from mucous neck cell to chief cell. The secretory granules of the chief cell were hardly labeled. we also found an intermediate cell belonging between mucous neck cell and chief cell. Since pepsinogen 1 is also found in these three types of cells, we concluded the mucous neck cell is the precursor cell of the chief cell.(3) Terminal glycosylation sites of the cell was studied in the colonic mucous cell. HPA, DBA, SBA stained the cis side of the Golgi apparatus, RCA-1 and PNA stained the trans side of the Golgi and UEA-1 and LFA statined the transmost elements of the Golgi apparatus. The facts which had been ascertained by the electron microscopic radioautography have been reconfirmed by the lectin cytochemistry.(4) Using GSA-11 agglutin, we observed the developmental staining changes of this lectin in the fundic gland. This lectin statined the covering epithelia and gland base at the embryo. After birth, this lectin stained basal to intermediate regions. This lection stains the mucous neck region at adult. The staining shift has been ascerteined in this lection during the development of the fundic gland.

近两年来，课题组开展了以下四个项目的研究：（1）以大鼠胃和结肠为模型器官，建立了标记凝集素的最佳染色条件。在我们的研究中，将胶体金标记的凝集素和亲水性树脂组合使用包埋后染色方法。在众多课题组中，我们课题组属于实验中使用多种凝集素的课题组。(2)利用上述染色条件，对大鼠洪迪腺的各种细胞类型进行了染色。我们观察到从粘液颈细胞到主细胞的标记强度降低。主细胞的分泌颗粒几乎没有标记。我们还发现了一个介于颈粘液细胞和主细胞之间的中间细胞。由于胃蛋白酶原1也存在于这三种类型的细胞中，因此我们推断粘液颈细胞是主细胞的前体细胞。(3)在结肠粘液细胞中研究了该细胞的末端糖基化位点。 HPA、DBA、SBA 对高尔基体的顺侧进行染色，RCA-1 和 PNA 对高尔基体的反侧进行染色，UEA-1 和 LFA 对高尔基体的反侧元件进行染色。电镜放射自拍照所确定的事实，又被凝集素细胞化学所证实。(4)利用GSA-11凝集素，观察了该凝集素在胃底腺的发育染色变化。这种凝集素抑制了胚胎的覆盖上皮和腺基。出生后，这种凝集素对基底至中间区域进行染色。这种分泌物会在成年时将颈部粘液区域染色。在眼底腺发育过程中，该样本中的染色变化已被确定。