Intermediate Cell Functions in Epidermal Development

表皮发育中的中间细胞功能

• 批准号: 9770766
• 负责人: Terry H Lechler
• 金额: $ 20.65万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2021-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9770766
• 关键词: Address; Adult; Animals; Basal Cell; Basement membrane; Cell Proliferation; Cell physiology; Cells; Development; Differentiation Antigens; Embryo; Embryonic Development; Environment; Epidermis; Epithelial; Epithelium; Family; Foundations; Gene Expression; Generations; Goals; In Vitro; Integrins; Ker10 protein; Longevity; Mediating; Mitotic; Morphogenesis; Mus; Pathway interactions; Population; Pregnancy; Premature Infant; Proliferating; Regenerative Medicine; Reporting; Role; Signal Transduction; Site; Skin; Specific qualifier value; Stem cells; Stratification; Stratum Basale; Testing; Therapeutic Uses; Time; Tissue Differentiation; Tissue Expansion; Tissues; Wound Healing; cell type; insight; mouse model; novel; tool; transcription factor; transcriptome

Abstract During embryonic development, the epidermis transforms from a single cell layer to a stratified, fully differentiated tissue that forms a barrier to the external environment. Stratification is driven by proliferation in both the basal cell layer and in a transient transit-amplifying cell population called intermediate cells. These cells express differentiation markers yet remain mitotic, and are hypothesized to promote tissue expansion. However, the roles for intermediate cells have never been directly addressed. Neither do we understand how these cells remain proliferative in an environment that normally promotes differentiation. Here we take advantage of novel mouse lines that we have developed to address roles of intermediate and spinous cells in the expansion and morphogenesis of the epidermis, and to determine how these cells remain mitotically active when not attached to the underlying basement membrane. These studies are expected to identify pathways to promote tissue expansion/maturation for pre-term infants and for regenerative medicine, as well as provide novel mechanistic basis for signaling between epidermal cell layers.

摘要 在胚胎发育过程中，表皮从单细胞层转变为 成层的、完全分化的组织，形成对外部环境的屏障。 分层是由基底细胞层和瞬时细胞层中的增殖驱动的。 一种叫做中间细胞的传递放大细胞群。这些细胞表达 分化标志物仍保持有丝分裂状态，并被假设促进组织 扩张.然而，中间细胞的作用从来没有直接 处理。我们也不知道这些细胞是如何在一个 通常促进分化的环境。在这里，我们利用小说 我们开发的小鼠品系，以解决中间细胞和棘细胞的作用， 在表皮的扩张和形态发生中，并确定这些细胞如何 当不附着在下面的基底膜上时保持有丝分裂活性。 这些研究有望确定促进组织 用于早产儿和再生医学的扩张/成熟，以及 为表皮细胞层之间的信号传导提供了新的机制基础。