MOLECULAR AND CELL BIOLOGICAL STUDY ON CELLULAR RESPONSE TO MECHANICAL STRESS

细胞对机械应力反应的分子和细胞生物学研究

• 批准号: 12670032
• 负责人: TAKEMASA Tohru
• 金额: $ 1.92万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670032/
• 关键词: MECHANICAL STRESS; STRESS FIBER; ACTIN; BIOMECHANICS; MOLECULAR AND CELLULAR BIOLOGY; PATENT; CYCLIC STRAIN; EXERCISE; 転写

The stress fiber is one of the most prominent cytoskeletal components that contribute to the maintenance of cell architecture. It has generally been believed that upon cyclic stretching, both cells and their stress fibers become perpendicularly aligned to the direction of stretching. However, using our newly developed stretching device, we have recently found the contrary evidence that stress fibers in endothelial cells rapidly become rearranged at a specific oblique angle relative to the direction of stretching. In light of this finding, we attempted to establish the explanation for such a phenomenon either experimentally or theoretically. It was thus revealed that stress fibers align at a particular angle so as to minimize their length alterations in cyclic deforming fields. Rearrangement of the stress fibers at this angle probably occurs as a result of avoiding compressive stress and may be interpreted as a way of reducing the mechanical stress to which. they are subjected during the deformation. This hypothesis explains well the reason not only for the survival of the stress fibers at a particular oblique angle, but also for the reduced numbers of stress fibers found, at the other angles on cyclic deforming fields. Moreover, theoretical study supports this hypothesis of intracellular stress fiber orientation under cyclic deformation.We also expand our study from microscopic level to macroscopic level, that is, individual human being. Exercise with skeletal muscle contraction can be considered to mechanical stress from the standpoint of cells in body. We studied cellular response to both endurance and resistance exercise.

应力纤维是维持细胞结构的最重要的细胞骨架成分之一。一般认为，在循环拉伸时，细胞和它们的应力纤维都垂直于拉伸方向排列。然而，使用我们新开发的拉伸装置，我们最近发现了相反的证据，即内皮细胞中的应力纤维在相对于拉伸方向的特定斜角处迅速重排。根据这一发现，我们试图从实验或理论上解释这种现象。结果表明，应力纤维以特定的角度排列，从而使其在循环变形场中的长度变化最小。应力纤维在这个角度的重新排列可能是由于避免了压缩应力而发生的，并且可以被解释为减少机械应力的一种方式。它们在变形过程中受到影响。这一假设很好地解释了不仅在特定的斜角处应力纤维存活的原因，而且在循环变形场的其他角度处发现的应力纤维数量减少的原因。此外，理论研究也支持了循环变形下细胞内应力纤维取向的假设，并将研究从微观层面扩展到宏观层面，即人类个体。从机体细胞的角度来看，骨骼肌收缩运动可视为机械应力。我们研究了细胞对耐力和阻力运动的反应。

项目成果

Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi: "Theoretical study of intracellular stress fiber orientation under cyclic deformation"Journal of Biomechanics. 33・11. 1501-1505 (2000)

Hiroshi Yamada、Tohru Takemasa、Takami Yamaguchi：“循环变形下细胞内应力纤维取向的理论研究”生物力学杂志33・11（2000）。

Hioshi Yamada et al.: "Theoretical study of inhacethar stress fiber onentation under cyclic deformation"Jouranl of Biomechanics. 33(11). 1501-1505 (2000)

Hioshi Yamada 等：“循环变形下 Inhacethar 应力纤维方向的理论研究”生物力学杂志。

Hiroshi Yamada, Tohru Takemasa, Takami Yamaguchi(編著者の一人): "Clinical Application of Computational Mechanics for the Cardiovascular System"Springer-Verlag Tokyo. 382 (2000)

Hiroshi Yamada、Tohru Takemasa、Takami Yamaguchi（编辑之一）：“心血管系统计算力学的临床应用”Springer-Verlag Tokyo 382 (2000)。

Keiji Sugimato et al.: "Detection of intracellular mtricoxide using combination of aldehyde fixafives with 4-5-damino fluorescein diacetate"Histochemistry and cell Biology. 113. 341-347 (2000)

Keiji Sugimato 等人：“使用醛固定剂与 4-5-damino 荧光素二乙酸酯的组合检测细胞内氧化酶”组织化学和细胞生物学。

Keiji Sugimoto, Sachiko Fujii, Tohru Takemasa, Kazuo Yamashita: "Detection of intracellular nitric oxide using a combination of aldehyde fixatives with 4,5-diaminofluorescein diacetate"Histochemistry and Cell. 113. 341-347 (2000)

Keiji Sugimoto、Sachiko Fujii、Tohru Takemasa、Kazuo Yamashita：“使用醛固定剂与 4,5-二氨基荧光素二乙酸酯的组合检测细胞内一氧化氮”组织化学和细胞。