Association Between Serum Cytokine Concentration And Between-subject variability in Airway Obstructive Response In Welders.

本研究的目的是在控制了磁肺成像(MPG)估计的累积暴露水平后，评估电焊工血清中几种细胞因子水平的对象间和对象内的变异性，以及与焊接烟雾肺反应的个体变异性的关系。研究对象来自一家工厂约150名主要从事弧焊作业的工人。在征得每个参与者的知情同意后，我们在2000年和2001年利用高灵敏度的化学发光ELISA法检测了血清白介素4(IL4)、白介素6(IL6)、白介素8(11.8)和干扰素-γ(干扰素γ)的水平(Satoh T.等，1995年)。此外，我们还检测了血清和尿液中的其他几种生物标志物，如尿中8-羟基-2-脱氧鸟苷和可替宁水平。通过MPG和肺功能水平估计的累积暴露的纵向变化也被FI…评估基线检测后5年以上所有细胞因子分布范围较广，呈中度或重度正向偏斜。然而，在2000年的第一次测量和2001年的第二次测量之间，每种细胞因子都观察到显著的正相关。这些结果表明，在受试者内部，细胞因子的变异性并不像从受试者之间的横断面估计的变量中预期的那样大。因此，可以认为每个工人都有自己的细胞因子参考水平。另一个有趣的结果是血清IL-4和IL-8水平呈显著正相关(S等级相关系数=0.62)。这可能表明焊接烟雾对呼吸系统影响的共同方面。MPG测量显示，在五年的随访中，工人肺的磁化能力明显下降。据估计，烟雾的沉积平均减少了66%。因此，对工作环境进行充分的监管将有助于减少接触焊接烟雾和消除肺部已积累的颗粒物。目前正在详细研究血清细胞因子水平与累积暴露于焊接烟雾和肺功能变异性之间的关系。较少

The objective of this study was to evaluate between and within-subject variability of serum level of several types of cytokine among electric arc welders, in relation with individual variability of pulmonary response to welding fumes, after controlling for cumulative exposure levels estimated by magnetopneumography (MPG).Study subjects were derived from an occupational cohort which was consisted of about 150 workers mainly engaging in electric arc welding in a factory. After given a informed consent from each participant, we examined serum level of interleukin-4 (IL4), interleukin-6 (IL6), interleukin-8 (11.8), and interferon-γ (IFNγ) in 2000 and in 2001, utilizing high sensitivity chemiluminescence ELISA method (Satoh T. et al, 1995). In addition, we also measured several other biomarkers in serum and in urine, such as 8-hydroxy-2-deoxyguanosine and cotinine levels in urine. Longitudinal change in cumulative exposure estimate by MPG and pulmonary function levels were also evaluated fi … More ve years after the baseline measurementsAll cytokines distributed in wide ranges and showed moderate or severe skewness towards positive direction. However, significantly positive association was observed between the first measurements in 2000 and the second ones in 2001 in each cytokine. These results suggest that withinsubject variability of cytokines is not so large as expected from cross-sectionally estimated betweensubject variation. Thus, it might be possible to consider that each individual worker has his own reference level of cytokines. Another interesting result was significant positive association between serum IL4 and IL8 levels (Spearman s rank correlation coefficient=0.62). It might indicate common aspects of the influence of welding fumes on respiratory system. MPG measurements showed marked reduction in magnetizability of the workers lung in a five-year follow-up. It was estimated as 66% decrease in deposition of fumes on average. Therefore, sufficient regulation of the working environment will be helpful in reducing the exposure to welding fumes and eliminating particulates already accumulated in the lungs. The association of serum cytokine levels with cumulative exposure to welding fumes and pulmonary function variability is now under consideration in detail. Less