Association Between Serum Cytokine Concentration And Between-subject variability in Airway Obstructive Response In Welders.

焊工气道阻塞反应中血清细胞因子浓度与受试者间变异之间的关联。

• 批准号: 12670331
• 负责人: NAKADATE Toshio
• 金额: $ 1.92万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670331/
• 关键词: Serum cytokine; Forced Expiratory Volume in One Second; Magnetopneumography; Urine 8-Hydroxy-2'deoxyguanosine; Welding fume; Biomarker; Variability; Spirometry; 溶接作業; ヒューム

The objective of this study was to evaluate between and within-subject variability of serum level of several types of cytokine among electric arc welders, in relation with individual variability of pulmonary response to welding fumes, after controlling for cumulative exposure levels estimated by magnetopneumography (MPG).Study subjects were derived from an occupational cohort which was consisted of about 150 workers mainly engaging in electric arc welding in a factory. After given a informed consent from each participant, we examined serum level of interleukin-4 (IL4), interleukin-6 (IL6), interleukin-8 (11.8), and interferon-γ (IFNγ) in 2000 and in 2001, utilizing high sensitivity chemiluminescence ELISA method (Satoh T. et al, 1995). In addition, we also measured several other biomarkers in serum and in urine, such as 8-hydroxy-2-deoxyguanosine and cotinine levels in urine. Longitudinal change in cumulative exposure estimate by MPG and pulmonary function levels were also evaluated fi … More ve years after the baseline measurementsAll cytokines distributed in wide ranges and showed moderate or severe skewness towards positive direction. However, significantly positive association was observed between the first measurements in 2000 and the second ones in 2001 in each cytokine. These results suggest that withinsubject variability of cytokines is not so large as expected from cross-sectionally estimated betweensubject variation. Thus, it might be possible to consider that each individual worker has his own reference level of cytokines. Another interesting result was significant positive association between serum IL4 and IL8 levels (Spearman s rank correlation coefficient=0.62). It might indicate common aspects of the influence of welding fumes on respiratory system. MPG measurements showed marked reduction in magnetizability of the workers lung in a five-year follow-up. It was estimated as 66% decrease in deposition of fumes on average. Therefore, sufficient regulation of the working environment will be helpful in reducing the exposure to welding fumes and eliminating particulates already accumulated in the lungs. The association of serum cytokine levels with cumulative exposure to welding fumes and pulmonary function variability is now under consideration in detail. Less

本研究的目的是评估电弧焊工人中几种类型的细胞因子的血清水平的个体间和个体内变异性，以及与焊接烟雾肺反应的个体变异性的关系，在控制通过磁肺描记术（MPG）估计的累积暴露水平之后，研究对象来自一个职业队列，该队列由一家工厂的约150名主要从事电弧焊接的工人组成。于2000年和2001年，在获得每位参与者的知情同意后，采用高灵敏度化学发光ELISA法（SatohT.等人，1995）。此外，我们还测量了血清和尿液中的其他几种生物标志物，如尿液中的8-羟基-2-脱氧鸟苷和可替宁水平。通过MPG和肺功能水平评估累积暴露估计值的纵向变化， ...更多信息 基线测量后5年，所有细胞因子均呈宽范围分布，并呈中度或重度正向偏态。然而，在2000年的第一次测量和2001年的第二次测量之间观察到每种细胞因子的显着正相关。这些结果表明，受试者内细胞因子的变异性并不像预期的那样大。因此，可以考虑每个个体工人都有自己的细胞因子参考水平。另一个有趣的结果是血清IL 4和IL 8水平之间的显著正相关（斯皮尔曼等级相关系数=0.62）。这可能表明焊接烟尘对呼吸系统影响的共同方面。MPG测量结果显示，在五年的随访中，工人肺的磁化率明显降低。据估计，烟雾沉积平均减少66%。因此，充分调节工作环境将有助于减少焊接烟雾的暴露，并消除已积聚在肺部的颗粒物。血清细胞因子水平与累积暴露于焊接烟雾和肺功能变异性的关联现在正在详细考虑中。少

项目成果

Zheng Y., Saito M., Uchikawa Y, Kotani M., Nakadate T., Yagami T: "Role of magnetopneu mography measurement for prevention of pneumo-coniosis"Proceedings' of the 12th International Conference on Biomagnetism. 1019-1022 (2002)

郑Y.，齐藤M.，内川Y，小谷M.，中馆T.，八神T：“磁气压扫描测量对预防肺尘埃沉着病的作用”第12届国际生物磁学会议论文集。

Tsutsumi A., Kagawa J., Yamano Y., Nakadate T., Shimizu S: "Relation between cotinine in the urine and indices based on self-declared smoking habits."Environmental Health & Preventive Medicine. 6(4). 240-247 (2002)

Tsutsumi A.、Kakawa J.、Yamano Y.、Nakadate T.、Shimizu S：“尿液中的可替宁与基于自我声明的吸烟习惯的指数之间的关系。”环境健康

Tsutsumi, A., Kagawa, J., Yamano, Y., Nakadate, T., Shimizu, S.: "Relation between cotinine in the urine and indices based on self-declared smoking habit"Environmental Health and Preventive Medicine. 6. 240-247 (2002)

Tsutsumi, A.、Kakawa, J.、Yamano, Y.、Nakadate, T.、Shimizu, S.：“尿液中的可替宁与基于自我声明的吸烟习惯的指数之间的关系”环境健康与预防医学。

中館俊夫, 足立知永子, 他: "職業曝露作業者における血清サイトカイン値の変動性"日本衛生学雑誌. 56. 369-369 (2001)

Toshio Nakadate、Chieko Adachi 等人：“职业暴露工人血清细胞因子水平的变异性”日本卫生杂志 56. 369-369 (2001)。

Nakadate T., Yagami X, Zheng Y., Kotani M., Nishida A: "Longitudinal change in magnetopneu mographic measurements in Japanese arc weld ers, in relation with working conditions, pulmonary function, and chest x-ray findings."Proceedings' of the 12th Interna

Nakadate T.、Yagami X、Zheng Y.、Kotani M.、Nishida A：“日本电弧焊工磁肺成像测量的纵向变化与工作条件、肺功能和胸部 X 光检查结果的关系。”会议记录