Epidemological study on the relationship between ischemia heart disease and anti-CagA antibody of Helicobacter pylori.

缺血性心脏病与幽门螺杆菌抗CagA抗体关系的流行病学研究

• 批准号: 12670334
• 负责人: MIYAZAKI Motonobu
• 金额: $ 2.18万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670334/
• 关键词: Helicobacter pylori; CagA; Ischemic heart disease

The purpose of this case-control study, carried out by examining for the presence of anti-CagA antibodies, was to investigate the relationship between ischemic heart disease (IHD) and Helicobacterpylori (H. pylori) infection. Enrolled subjects were 242 patients 94 controls. All cases were angiographically confirmed stenoses of coronary artery. Of 242 patients, patients with acute coronary syndrome (ACS) were 164. A control case is with the result of 'within normal limit' on coronary angiography. H. pylori seropositivity was determined with an IgG-specific enzyme II assay kit (Biomerica, Newport Beach, CA). Antibodies to CagA were determined with an enzyme-linked immunosorbent assay (ELISA). The antigen was a recombinant CagA antigen, which was supplied by Dr. Antonello Covacci (Immunobiological Research Institute, Siena, Italy). The logistic regression model was applied to analyze a relationship between an ACS and a control in regard to H. pylori seropositivity. Odds ratios (ORs) and their 95% confidence intervals (95% CIs) were determined to evaluate the strength of the association. Probability values less than 0.05 were considered significant. The data were analysed with the Statistical Analysis System (SAS Institute, Gary, NC). The seropositivity for H. pylori in ACS group was 70.4% and it in controls was 72.1%. No statistically significant difference was observed (OR= 0.92, 95% CI ; 0.51-1.67, p= 0.073). Of 100 H. pylori-seropositive ACS cases, 64.0% was CagA positive and in 62 H. pylori-serbpositive controls, 66.1% was CagA positive. There was not statistically significant difference about the anti-CagA antibodies (OR= 0.91, 95% Cl ; 0.47-1.77, p= 0.783).

这项通过检测抗CagA抗体的病例对照研究的目的是调查缺血性心脏病(IHD)和幽门螺杆菌(H.Pylori)感染之间的关系。入选对象为242例患者和94例对照。所有病例均经冠状动脉造影证实为冠状动脉狭窄。242例患者中，急性冠脉综合征患者164例。一例对照病例的冠状动脉造影结果为“在正常范围内”。用免疫球蛋白特异性酶II试剂盒检测幽门螺杆菌血清阳性率(加利福尼亚州新港海滩Bimerica)。抗CagA抗体检测采用酶联免疫吸附试验(ELISA)。该抗原为重组CagA抗原，由意大利锡耶纳免疫生物学研究所Antonello Coacci博士提供。Logistic回归模型用于分析急性冠脉综合征和对照之间关于幽门螺杆菌血清阳性的关系。确定优势比(OR)及其95%可信区间(95%CI)来评估这种关联的强度。概率值小于0.05被认为是显著的。使用统计分析系统(SAS研究所，加里，北卡罗来纳州)对数据进行分析。急性冠脉综合征组幽门螺杆菌阳性率为70.4%，对照组为72.1%。两组间差异无统计学意义(OR=0.9295%CI；0.51~1.67，P=0.073)。在100例Hp阳性的急性冠脉综合征患者中，CagA的阳性率为64.0%，在62例Hp阳性的对照组中，CagA的阳性率为66.1%。抗CagA抗体阳性率差异无统计学意义(OR=0.91，95%CI；0.47~1.77，P=0.783)。