A follow-up study on interactive effects of genetic factors and dietary habits on trends of blood pressure and bone mass among pregnant women

在2000年至2002年的三年研究项目中，我们进行了三项不同的研究。首先，在2000年2月至2000年6月期间，76名日本妇女被确认怀孕，随后经历了正常的怀孕和分娩，并可在位于东京都的妇产科诊所中心随访至分娩后6个月，使用定量超声方法测量跟骨骨量作为骨评估指数（OSI）。<th>在随访期间OSI的个体内变化进行了分析后，OSI或OSI从妊娠到产后的变化的关系，以140项半定量食物频率问卷获得的营养和食物摄入量的估计进行了分析，使用逐步多元回归分析。其次，对于上述第一次调查中涉及的相同受试者，营养素摄入和/或血管紧张素原T174M基因型（AGTT 174）与营养素摄入和/或血管紧张素原T174M基因型（AGTT 174）之间的相关性， 关于我们 方法：对1999~2000年10月在我院住院的100例孕妇，分别于妊娠第1次、妊娠36个月、分娩后1个月测量血压，并分析其血压变化情况。<th><st>第三，对日本三户市及周边地区中老年女性居民线粒体DNA 5178A/C多态性的"A"型和"C"型之间的几种与衰老或动脉粥样硬化相关的生物标志物水平进行比较（Mt5178A/C），以调查Mt5178A/C对日本女性健康风险的潜在作用。主要结果和结论第一项研究：妊娠期和/或产后铁、维生素D、水果和蔬菜的摄入量与OSI从妊娠期到产后的变化呈正相关，而与妊娠期或产后胡萝卜素、氯化钠和肉类的摄入量呈负相关。这些结果表明，足够的铁，维生素D，或水果和蔬菜的摄入量可能是重要的保存后的骨量deliveration.Second调查：虽然所有的对象的血压水平都在正常范围内，在所有的三个调查点的平均血压略高于AGTT 174 M中TT型的M基因携带者。妊娠期血压变化与维生素C摄入量呈显著负相关，与氯化钠或钙摄入量呈显著正相关。胡萝卜素的摄入似乎对孕妇的血压有有益的影响。AGTT174M与饮食习惯对血压或血压变化的相互作用尚不明确。第三次调查：发现Mt5178A/C的A型（长寿型）血清甘油三酯或白蛋白水平明显低于C型（非长寿型）。Mt5178A/C也可能是孕妇健康风险的重要决定因素。少

In the present study project for three years between 2000 and 2002, we conducted three different studies. First, for seventy-six Japanese women who were assured of pregnancy between February 2000 and June 2000, subsequently experienced normal pregnancy and labor, and could be followed up until 6^<th> month after delivery at an obstetrics and gynecology clinic center located in Tokyo Metropolitan, the calcaneous bone mass was measured as osteo-assenssment index (OSI) using the quantitative ultrasound method. After the intra-individual changes in the OSI during the follow-up period were analyzed, the relations of the OSI or OSI change from gestation to postpartum to the estimates of nutrient and food intake obtained by a 140-item semi quantitative food-frequency questionnaire were analyzed using a stepwise multiple regression analysis. Secondly, for the same subjects involved in aforementioned first survey, associations of nutrients intake and / or angiotensinogen T174M genotype (AGTT174 … More M) with blood pressure or blood pressure changes during the investigation period were analyzed on the basis of blood pressure levels measured at first *osis of pregnancy, 36^<th> month of gestation and 1^<st> month after delivery. Thirdly, for middle and old-aged female residents in Mito-shi, Japan or *rrounding areas, several bio-marker levels relating to aging or atherosclerosis were compared between 'A' type and 'C" type of mitochondrial DNA 5178A/C polymorphism (Mt5178A/C) in order to investigate the potential roles of Mt5178A/C on health risks in Japanese women.Main results and conclusionsThe first study: Intake of iron, vitamin D, and fruits and vegetables during gestation and/or postpartum were positive correlates with the OSI change from gestational to postpartum, whereas it was inversely related to intake of carotene, sodium chloride and meats in gestation or postpartum. These results suggest that sufficient intake of iron, vitamin D, or fruits and vegetables may be important for preservation of bone mass after delivery.The second survey: Although blood pressure levels were all within normal range among subjects, mean blood pressure was slightly higher in M alle carriers than TT type in AGTT174M at all of the three investigation points. Blood pressure change during gestation was related significantly inversely to vitamin C intake and significantly positively to sodium chloride or calcium intake during gestation. Carrotene intake appeared to have beneficial effects on blood pressure among the pregnant women. The interactions of AGTT174M and dietary habits on blood pressure or blood pressure change were not clear.The third survey: It was found that serum triglycerides or albumin levels were significantly lower in A type (longevity type) than in C type (non-longevity type) of Mt5178A/C. Mt5178A/C might also be an important determinant of health risks in pregnant women. Less