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THE DEVELOPMENT OF AN ATTACHING AND EFFACING LESION BY AN ENTEROHAEMORRHAGIC ECHERICHIA COLI IN VIVO

肠出血性大肠杆菌体内附着和脱落病变的形成

基本信息

批准号：
12670502
负责人：
SUEYOSHI Masao
金额：
$ 2.11万
依托单位：
MIYAZAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670502/
关键词：
intestine Escherichia coli eaeA stx cattle

项目摘要

Young chicken were inoculated orally with an attaching and effacing E. coli (O103 : H-), and an inoculated microorganisms were isolated from 3 hours to 3 months after the inoculation. The adhesion of the microorganisms to the intestines of the chicken and the attaching-effacing lesions were also observed. Furthermore, one hundred of the calves with diarrhea were observed pathologically.AEEC was isolated from the cecal contents of all the chickens from 3 hrs until the first day in the infection experiment with the average 8.67 ± 0.41 (log_<10>CFU/g ± SD). Adhesion to the intestinal surface of AEEC was recognized from 3 hrs until 3 wks after the inoculation. The AE lesion was recognized from 9 hrs until 3 wks after the inoculation. AEEC was found on the surface of slight cellular the early stages of infection. The intracellular-filament and microvilli were expanded, and the microorganisms that attached to the mucosal surface were surrounded in the microvilli. And, its microvilli were disappeared and the Cup-formation and the pedestal protrusion were recognized as a cell membrane. The AE lesions expanded into the net-shaped to surround the crypt. AEEC left mucous membrane at the infection latter term, and the mucous membrane was repaired. The stx 1 gene (20/83), stx 1 + 2 gene (2/83) and an eaeA gene (9/83) were detected by the PCR method in the intestinal contents of calves with diarrhea, and the rate of the stx 1 genetic possession strain was very high.

对幼鸡口服接种附着性和消除性大肠杆菌(O103：H-)，并在接种后3小时至3个月内分离接种的微生物。还观察了微生物在鸡肠道上的粘附情况以及附着-消失的病变情况。此外，对100头腹泻犊牛进行病理观察。从感染实验3小时至第一天所有鸡的盲肠内容物中分离出AEEC，平均值为8.67±0.41(log_ 10 CFU/g±SD)。接种后 3 小时至 3 周，可观察到 AEEC 与肠表面的粘附。 AE损伤在接种后9小时至3周内被识别。感染早期在微小细胞表面发现AEEC。细胞内丝和微绒毛扩张，附着在粘膜表面的微生物被微绒毛包围。并且，其微绒毛消失，并且杯形和基座突起被识别为细胞膜。 AE病灶扩展成网状，包围隐窝。 AEEC在感染后期留下了粘膜，并且粘膜得到了修复。采用PCR法在腹泻犊牛肠道内容物中检测到stx 1基因(20/83)、stx 1+2基因(2/83)和eaeA基因(9/83)，stx 1基因拥有株率很高。