CELL-TO-CELL INTERACTION IN THE MARGINALSINUS OF MESENTERIC LYMPH NODE
肠系膜淋巴结边缘的细胞间相互作用
基本信息
- 批准号:12670515
- 负责人:
- 金额:$ 1.98万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
1) Distribution of lymphatic microvessels and migration of lymphocyte in the mesenteric lymph node of ratLabeled lymphocytes in blood adhered to Peyer's patch venules preferentially. The lymphocytes transmigrated from the venules and entered efferent lymphatics in Peyer's patches, and the flowed into the lymph node. The lymphocytes were arrested in the marginal sinus, which has huge surface sufficient for lymphocytes pooling. It is indicated that lymphocytes homing to mesenteric lymph nodes predominantly passes through afferent lymphatics.2) Visualization of macrophage migration in mesenteric lymph nodeFluorescent particles were taken up by EDS positive macrophages beneath the marginal sinus. The number of the particleladen macrophages seen by epi-illumination increased up to 12 hr after injection, and then declined. Some particle-laden macrophages entered the marginal sinus and flowed rapidly toward the hilus of the lymph node. In cryostat sections, the particle-laden macrophages tran … More smigrated through the cortex toward the medulla in course of time. Upon phagocytosis of foreign bodies, the ED3 positive macrophages migrate toward the medulla through the marginal sinus or cortex to find immunoproliferative microenvironment.3) Experimental animal model of early lymph node metastasis and response of antigen presenting cells.Fluorescence labeled cancer cells (AH 130 ascites hepatoma cells) were arrested in the marginal sinus of the meso-cecum lymph node. The number of cancer cells, flowed into the marginal sinus, reached the maximum 6 hours after injection. The marginal sinus was filled with cancer cells and expanded 4days after injection. Beneath the cancer cell linings, ED 3 positive macrophages have accumulated. They incorporated apoptotic cancer cells. The dendritic cell, resided in the paracortex, did not accumulate, but distributed in small number in the rim of the cancer cell cluster. In lymph nodes depleted of macrophages, the cancer cells invaded early to the paracortex. Macrophages beneath the marginal sinus play a role in the first line defense against cancer proliferation and invasion.4) Migration of dendritic cells from the gut to lymph node.Labeled dendritic cells were seen to migrate to the mesenteric lymph node 6 hours after injection to the cecum submucosa, and reached the maximum in number at 48 hours, and then declined at 72 hours. Cytokines stimulation in vitro upregulated the expression of MHC class II of dendritic cells, resulting in early transfer to the lymph node. Less
1)大鼠肠系膜淋巴结微淋巴管的分布及淋巴细胞的迁移标记的淋巴细胞优先粘附于派尔结小静脉。淋巴细胞从淋巴结的小静脉进入淋巴结的输出淋巴管,流入淋巴结。淋巴细胞滞留在边缘窦中,边缘窦具有足够的表面用于淋巴细胞汇集。2)巨噬细胞在肠系膜淋巴结内迁移的观察在边缘窦下可见EDS阳性的巨噬细胞摄取荧光颗粒。在注射后12小时,由落射照明观察到的颗粒状巨噬细胞的数量增加,然后下降。一些携带颗粒的巨噬细胞进入边缘窦并迅速流向淋巴结门。在低温恒温切片中,携带颗粒的巨噬细胞通过 ...更多信息 随着时间的推移,从皮质向髓质迁移。ED 3阳性巨噬细胞吞噬异物后,通过边缘窦或皮质向髓质迁移,寻找免疫增殖微环境。3)早期淋巴结转移和抗原提呈细胞应答的实验动物模型:荧光标记的癌细胞(AH 130腹水肝癌细胞)被阻滞在盲肠系膜淋巴结边缘窦。癌细胞流入边缘窦的数量在注射后6小时达到最大值。注药后4d,边缘窦内癌细胞浸润,并扩大。在癌细胞内衬下,艾德3阳性巨噬细胞已经积聚。它们掺入了凋亡的癌细胞。树突状细胞位于副皮质区,不聚集,少量分布于癌细胞团边缘。在巨噬细胞耗尽的淋巴结中,癌细胞早期侵入副皮质。边缘窦下的巨噬细胞在对抗肿瘤增殖和侵袭的第一线防御中发挥作用。4)肠内树突状细胞向淋巴结的迁移盲肠粘膜下层注射后6h可见标记的树突状细胞向肠系膜淋巴结迁移,48h数量达到最大值,72h数量下降。细胞因子刺激在体外上调树突状细胞的MHC II类的表达,导致早期转移到淋巴结。少
项目成果
期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
岸川浩: "物理的圧力負荷による小腸上皮細胞の増殖およびサイトカイン放出の検討"Cytoprotection & biology. 18. 49-51 (2000)
Hiroshi Kishikawa:“由于物理压力负荷而检查小肠上皮细胞的增殖和细胞因子释放”《细胞保护与生物学》18. 49-51 (2000)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Nagata Hiroshi: "In vivo identification of parasinus macrophage in the mesenteric lymph node"Clinical Hemorheology and Microcirculation. 22(発表予定). (2000)
Hiroshi Nagata:“肠系膜淋巴结中副窦巨噬细胞的体内鉴定”临床血液流变学和微循环22(即将发表)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Nagata H et al: "Lymphocyte homing pathway to mesenteric lymph node"Microcirculation Annual. 16. 83-84 (2000)
Nagata H等人:“肠系膜淋巴结的淋巴细胞归巢途径”微循环年鉴。
- DOI:
- 发表时间:
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- 影响因子:0
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Nagata H et al: "Visualization of macrophage migration in mesenteric lymph node of rat"Microcirculation annual. 17. 139-140 (2001)
Nagata H等人:“大鼠肠系膜淋巴结中巨噬细胞迁移的可视化”微循环年度。
- DOI:
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- 期刊:
- 影响因子:0
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Hokari Ryota: "Involvement of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in the pathogenesis of granulomatous colitis in rats"Clin Exp Immunol. 126. 259-265 (2001)
Hokari Ryota:“粘膜地址素细胞粘附分子 1 (MAdCAM-1) 参与大鼠肉芽肿性结肠炎的发病机制”Clin Exp Immunol。
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- 影响因子:0
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NAGATA Hiroshi其他文献
NAGATA Hiroshi的其他文献
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{{ truncateString('NAGATA Hiroshi', 18)}}的其他基金
Relationship between lupus band and anti-basement membrane antibodies in cutaneous lupus erythematosus
皮肤红斑狼疮狼疮带与抗基底膜抗体的关系
- 批准号:
25860975 - 财政年份:2013
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Judgments of the grammaticality of sentences violating Chomsky's principle of Full Interpretation
违反乔姆斯基全面解释原则的句子语法判断
- 批准号:
15530471 - 财政年份:2003
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
MECHANISM OF REGULATING MOBILIZATION AND MIGRATION OF ANTIGEN PRESENTING CELLS DURING INFLAMMATION
炎症过程中抗原呈递细胞动员和迁移的调节机制
- 批准号:
14570507 - 财政年份:2002
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Analyses for association between nasal allergy and candidate genes for atopy
鼻过敏与特应性候选基因之间的关联分析
- 批准号:
12671650 - 财政年份:2000
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
An assimilation effect in judging the grammaticality of sentences violating the subjacency condition
判断违反相邻条件句子语法的同化效应
- 批准号:
09610082 - 财政年份:1997
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Screening for genetic polymorphisms of histamine H1 and NK1 receptors which are involved in development of hyperreactivity of the nose
筛选与鼻子高反应性发展相关的组胺 H1 和 NK1 受体的遗传多态性
- 批准号:
08671951 - 财政年份:1996
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanisum of the Separation of the Unsteady Boundary Layer on the Moving Cylinder in a Uniform Flow
均匀流运动圆柱上非定常边界层的分离机理
- 批准号:
62550126 - 财政年份:1987
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Studies on the relationships between ecotypic characteristics and distribution of some important trees in Japan
日本一些重要树木的生态型特征与分布关系的研究
- 批准号:
61304022 - 财政年份:1986
- 资助金额:
$ 1.98万 - 项目类别:
Grant-in-Aid for Co-operative Research (A)
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