Effects of cerebellar and globus pallidusstimulation in epilepsy

小脑和苍白球刺激对癫痫的影响

• 批准号: 12670626
• 负责人: HORI Ariyuki
• 金额: $ 0.64万
• 依托单位: Kanazawa Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670626/
• 关键词: cerebellar stimulation; anti-epileptic effects; kindling; globus pallidus stimulation; globus pallidus pars interna; thalamo-cortical pathway; status epilepticus; Kainic acid

(1) Effects of cerebellar stimulation in kindled ratsElectrical stimulation of cerebellum was clinically introduced to control epilepsy and movements disorders in 1973. The efficacy of cerebellar stimulation on epilepsy and animal models of epilepsy, however, is still controversial. If cerebellar stimulation has antepileptic effects, transcranial magnetic stimulation, which is used clinically recently, might be avaable to suppress seizure. The aim of this study was to examine the antiepileptic effects of cerebellar stimulation.Electrodes were implanted to basolateral amygdala and contralateral cerebellum, stereotaxically under anesthesia in SD rats. Cerebellar simulation was delivered once a day in Rats fully kindled. Total 40 times cerebellar stimuli were given. Rats showed elevated afterdischarge threshold and reduced seizure stage after cerebellar stimulation. These antiepileptic effects, however, were transient. Intermittent cerebellar stimulation could not be available treatment to suppress seizure permanently. Further studies are needed to apply the transcranial magnetic stimulation as treatment of seizure.(2) Effects of globus pallidus pars interna (GPi) stimulation in status epilepticus(SE)GPi has inhibitory input to thalamus, which is important pathway at secondary generalization in SE. The efficacy of GPi stimulation on SE was studied. If GPi has anti-epileptic effects especially in SE, new treatment for SE might be established. The aim of this study was to examine the anti-epileptic effects of GPi.Electrodes were implanted to GPi in SD rats. SE was induced by Kainic acid injection intraperitoneally. Kainic acid injection induced SE with stage 5 seizure originated with limbic seizures. GPi stimulation was delivered continuously during SE GPi stimulation decreased severity of seizure stage and frequency of stage 5 seizures. GPi stimulation might be effective tosuppress the thalamo-cortical activation diring secondary generalized SE.

(1)电刺激小脑对点燃大鼠的作用1973年电刺激小脑被引入临床用于控制癫痫和运动障碍。然而，小脑刺激对癫痫和癫痫动物模型的有效性仍然存在争议。如果小脑刺激具有抗癫痫作用，那么最近临床上使用的经颅磁刺激可能有抑制癫痫发作的作用。本研究在麻醉状态下，将电极植入SD大鼠杏仁基底外侧核和对侧小脑，观察小脑刺激的抗癫痫作用。在完全点燃的大鼠中每天递送一次小脑模拟。共刺激小脑40次。刺激小脑后，大鼠的后放电阈值升高，癫痫发作期缩短。然而，这些抗癫痫作用是短暂的。间歇性小脑刺激不能永久抑制癫痫发作。经颅磁刺激作为癫痫治疗手段的应用尚需进一步研究。(2)苍白球内侧部（GPi）刺激对癫痫持续状态（SE）的影响苍白球内侧部（GPi）对丘脑有抑制性输入，是SE继发泛化的重要通路。研究了GPi刺激对SE的功效。如果GPi具有抗癫痫作用，特别是在SE中，则可能建立SE的新治疗方法。本实验采用SD大鼠GPi电极植入法，观察GPi的抗癫痫作用。腹腔注射红藻氨酸诱发SE。海人酸注射诱发SE伴5期癫痫发作，起源于边缘系统癫痫发作。在SE GPi刺激期间连续递送GPi刺激降低了癫痫发作阶段的严重程度和5级癫痫发作的频率。刺激GPi可有效抑制继发性SE时丘脑-皮层的激活。

项目成果

堀有行: "キンドリングてんかんモデルにおける小脳電気刺激のてんかん閾値およびてんかん症状に及ぼす影響"てんかん治療研究振興財団研究年報. 12. 89-92 (2000)

Yuyuki Hori：“小脑电刺激对点燃癫痫模型中癫痫阈值和癫痫症状的影响”癫痫治疗研究基金会年度报告 12. 89-92 (2000)。

堀 有行: "キンドリングてんかんモデルにおける小脳電気刺激のてんかん閾値およびてんかん症状に及ぼす影響"てんかん治療研究振興財団研究年報. 12. 89-92 (2000)

Yuyuki Hori：“小脑电刺激对点燃癫痫模型中癫痫阈值和癫痫症状的影响”癫痫治疗研究基金会年度报告 12. 89-92 (2000)。

Ariyuki Hori, Genjiro Hirose: "Effects of cerebellar stimulation in kindled rats"Ann. Rep. Jpn. Epi. Res. Found.. 12. 8992 (2000)

Ariyuki Hori、Genjiro Hirose：“小脑刺激对点燃大鼠的影响”Ann。