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The analysis and clinical application cellular characteristics of lung carcinoma or thyroid cancer using radionuclide tracer

放射性核素示踪剂对肺癌或甲状腺癌细胞特征的分析及临床应用

基本信息

批准号：
12670914
负责人：
ISHIBASHI Masatoshi
金额：
$ 1.54万
依托单位：
KURUME UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Although thallium-201 (^<201>Tl) uptake is related to perfusion in many normal tissues, the biologic rationale for ^<201>Tl uptake in tumors is uncertain. To determine if tumor uptake is related to cell proliferation, we correlated the relative retention of ^<201>Tl in lung tumors with expression of Ki-67, an indicator of cell proliferation. Methods : Sixty patients with lung tumors, included small cell carcinoma (n=8) and non-small cell carcinoma (n=52), underwent ^<201>Tl single photon emission computed tomography (SPECT) imaging. The ^<201>Tl lesion uptake was determined on early and delayed images and the radiotracer retention index (RI) was calculated. Tumor specimens were obtained at surgery or bronchoscopy. The cell proliferation ratio was estimated using MIB-1, a monoclonal antibody that recognized the nuclear antigen Ki-67.The average ^<201>Tl index was 2.13 + 0.61 (early) and 2.46 ± 0.83 (delayed). The average RI was 17.44 ± 35.01. Overall, the ^<201>Tl index (delayed) and the cancer cell proliferation were correlated (r=0.70, p<.0001). Of interest, there was a significant correlation (r=.872, p<.0005) between the ^<201>Tl index on delayed images and cell proliferation ratio in patients with small cell but not non-small cell lung carcinoma. The ^<201>Tl index (delayed) was significantly higher (p<.0001) for patients with small cell lung carcinoma than for patients with non-small cell lung carcinoma. In conclusion, ^<201>Tl imaging appears to be useful for evaluating patients with small cell lung carcinoma but not non-small lung carcinoma, and is correlated with the monoclonal antibody MIB-1, a marker for cell proliferation.Next step, we have evaluated the cell proliferation and rate of proliferation for patients with non-small lung carcinoma or thyroid cancer using the specific antibody recognized to MAPK/ERK. The resultant data obtained by us can be presented within a couple of months.

尽管在许多正常组织中铊-201 （^<201>Tl）摄取与灌注有关，但肿瘤中铊-201 （^<201>Tl）摄取的生物学原理尚不确定。为了确定肿瘤摄取是否与细胞增殖有关，我们将肺肿瘤中^<201>Tl的相对保留与细胞增殖指标Ki-67的表达联系起来。方法：60例肺肿瘤患者，包括小细胞癌（n=8）和非小细胞癌（n=52），行^<201>Tl单光子发射计算机断层扫描（SPECT）。在早期和延迟图像上确定^<201>Tl病变摄取，并计算放射性示踪剂保留指数（RI）。肿瘤标本在手术或支气管镜检查中获得。使用识别核抗原Ki-67的单克隆抗体MIB-1估计细胞增殖率。平均^<201>Tl指数为2.13 + 0.61（早期）和2.46±0.83（延迟）。平均RI为17.44±35.01。总体而言，^<201>Tl指数（延迟）与癌细胞增殖相关（r=0.70, p< 0.0001）。有趣的是，在小细胞肺癌而非非小细胞肺癌患者中，延迟图像上的^<201>Tl指数与细胞增殖率之间存在显著相关性（r= 0.872, p< 0.0005）。小细胞肺癌患者的^<201>Tl指数（延迟）显著高于非小细胞肺癌患者（p< 0.0001）。综上所述，^<201>Tl成像似乎可用于评估小细胞肺癌而非非小细胞肺癌患者，并且与单克隆抗体MIB-1（细胞增殖标志物）相关。下一步，我们将使用MAPK/ERK识别的特异性抗体评估非小肺癌或甲状腺癌患者的细胞增殖和增殖率。我们获得的最终数据可以在几个月内提交。