Behavioral and neuropharmacological study on the cellular expression of transcriptional factors in rat basal ganglia

1)我们研究是否L-DOPA，可能会增加生产的羟基自由基在完整的和多巴胺去神经大鼠纹状体。水杨酸捕获结合体内微透析提供了作为羟基自由基产生标志物的2，3-二羟基苯甲酸（2，3-DHBA）的测量。结果表明，重复给予高剂量L-DOPA可增加去多巴胺神经纹状体内羟自由基的产生。通过腹膜内注射安非他明激活表达Fos免疫反应性。纹状体传出活性抑制纹状体内注入的反义寡核苷酸针对即时早期基因，c-fos的信使RNA。这种抑制产生了鲁棒的旋转行为， 关于我们 安非他明刺激后同侧GP和EP中Fos的非典型表达。定量分析表明，大多数的安非他明激活的神经元在GP和EP表达GABA。本研究结果表明，这两个核团的投射神经元的抑制作用受纹状体输出活动的调节。3）为了阐明6-羟基多巴胺损伤大鼠胚胎腹侧中脑（VM）纹状体内移植物“发育”过程中离子型谷氨酸受体免疫阳性细胞的形态学变化，免疫组化检测表达N-甲基-D-天冬氨酸（NMDA）受体亚单位1（NR 1）的细胞，移植后1、4和12周纹状体内VM移植物中的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸酯（AMPA）受体亚单位（GluR 1、GluR 2/3和GluR 4）或酪氨酸羟化酶（TH）。结果表明，离子型谷氨酸受体在纹状体内VM移植物的发育过程中具有不同的作用。少

1) We examined whether L-DOPA, might increase production of hydroxyl radicals in intact and dopamine-denervated rat striatum. Salicylate trapping combined with in vivo microdialysis provided measurements of 2, 3-dihydroxybenzoic acid (2, 3-DHBA) as a marker of hydroxyl radical production. The results indicated that repeated administration of high dose L-DOPA increased production of hydroxyl radicals in dopamine-denervated striatum.2) Double immunostaining for Fos and γ-aminobutyric acid (GABA) was used in a previously established animal model of striatal dysfunction to examine whether GABA-immunoreactive neurons in the globus pallidus (GP) and entopeduncular nucleus (EP) are activated to express Fos immunoreactivity by intraperitoneal injection of amphetamine. Striatal efferent activity was suppressed by intrastriatal infusions of antisense oligodeoxynucleotide targeted to the messenger RNA of the immediate early gene, c-fos. This suppression produced robust rotational behavior and an … More atypical expression of Fos in the ipsilateral GP and EP following amphetamine challenge. Quantitative analysis revealed that a majority of the amphetamine-activated neurons in the GP and EP express GABA. The present results suggest an inhibition by projection neurons of the two nuclei that are regulated by striatal output activity.3) To elucidate the morphological changes in immunopositive cells of ionotropic glutamate receptors within intrastriatal "developing" grafts of fetal ventral mesencephalon (VM) in 6-hydroxydopamine-lesioned rats, immunohistochemistry was performed to detect cells expressing N-methyl-D-aspartate (NMDA) receptor subunit 1 (NR1), the α-amino-3 -hydroxy-5 -methyl-4-isoxazolepropionate (AMPA) receptor subunits (GluRl, GluR2/3, and GluR4), or tyrosine hydroxylase (TH) in the intrastriatal VM grafts at 1, 4, and 12 weeks following transplantation. The results suggest that the ionotropic glutamate receptors have differential roles during the developmental period of the intrastriatal VM grafts. Less