Autologous fibrine glue that enhance the wound healing -Combined usage of autologous fibrinogen and autologous fibroblast-

促进伤口愈合的自体纤维蛋白胶 -自体纤维蛋白原和自体成纤维细胞的结合使用-

• 批准号: 12671150
• 负责人: SUZUKI Kazuya
• 金额: $ 1.66万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671150/
• 关键词: wound healing; biological glue; autologous blood; autologous fibroblast; fibrinogen; VEGF; 自己繊維芽細胞; 創傷治療

Both Autologous fibrin glue and Autologous cultured fibroblast were used in the tracheal anastomosis, gastric wall suturing sites and intestine anastomosis.Pathological examination revealed good wound healing in experimental group comparing the control group in which commercially available fibrin glue was used or no materials were used.Physiological examination by the measurement of bursting pressure showed highest pressure in the experimental group.Concentration of hydroxyproline contained in collagen was highest in experimental group, that means the production of collagen by the autologous fibroblasts.These results showed that combined usage of autologous fibrin glue and autologous cultured fibroblasts at the wounds is very useful for the healing process.This new method seems to be safe and be applied easily to the clinical usage.

自体纤维蛋白胶和自体培养成纤维细胞均用于气管吻合、胃壁缝合部位和肠吻合。病理检查结果显示，实验组创面愈合较市售纤维蛋白胶和不使用纤维蛋白胶的对照组好。生理检查爆破压力测量显示，实验组压力最高。胶原中羟基脯氨酸含量以实验组最高，说明胶原是由自体成纤维细胞生成的。结果表明，自体纤维蛋白胶与自体培养成纤维细胞在创面的联合应用对创面的愈合是非常有益的。该方法安全可靠，易于临床应用。

项目成果

Kazuya Suzuki: "Reconstruction of diaphragm using autologous fascia lata : an experimental study in dogs"Ann Thorac Surg. 74・1. 209-212 (2002)

Kazuya Suzuki：“使用自体阔筋膜重建隔膜：在狗中的实验研究”Ann Thorac Surg 74・1（2002）。

Yoshino A, Suzuki K, Urano T, Aoki K, Takada Y, Takada A: "Enhanced secretion of tissue plasminogen activator by simultaneous use of retinoic acid and ascorbic acid from tissue cultured gastroepiploic artery"Life Sciences. 70. 1461-1470 (2002)

Yoshino A、Suzuki K、Urano T、Aoki K、Takada Y、Takada A：“同时使用来自组织培养的胃网膜动脉的视黄酸和抗坏血酸增强组织纤溶酶原激活剂的分泌”生命科学。

Kita Y, Nogimura H, Ida M, Kageyama Y, Ohi S, Ito Y, Matsushita K, Takahashi T, Suzuki K, Kazui T, Hayashi S: "Combined therapy of CTLA4Ig-gene transfection with FTY720 administration in rat lung allografts"Transplant Proc. 34(5). 1437-1440 (2002)

Kita Y、Nogimura H、Ida M、Kageyama Y、Ohi S、Ito Y、Matsushita K、Takahashi T、Suzuki K、Kazui T、Hayashi S：“CTLA4Ig 基因转染与 FTY720 给药在大鼠同种异体肺移植物中的联合治疗”移植

Kazuya Suzuki: "Polymer sheet for covering tumors during endoscopic surgery"Surgical Endoscopy. 14. 305-307 (2000)

Kazuya Suzuki：“在内窥镜手术中用于覆盖肿瘤的聚合物片”外科内窥镜检查。

Suzuki K, Takahashi T, Ito Y, Asai K, Shimota H, Kazui T: "Reconstruction of diaphragm using autologous fascia lata : an experimental study in dogs"Ann Thorac Surg. 74(1). 209-212 (2002)

Suzuki K、Takahashi T、Ito Y、Asai K、Shimota H、Kazui T：“使用自体阔筋膜重建膈肌：狗的实验研究”Ann Thorac Surg。