Artificial Peritoneum for Cancer Research and Surgical Treatment

用于癌症研究和手术治疗的人工腹膜

• 批准号: 12671163
• 负责人: UCHIYAMA Akihiko
• 金额: $ 2.24万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671163/
• 关键词: peritoneum; artifical peritoneum; peritoneal carcinomtosis; IFN-_γ; gene transfection; peritoneal trnasplantation; INF-γ; 中皮細胞; 移植

Peritoneum is an physiologically important tissue which serve as barrier between peritoneal cavity and general circulation system. In the present study, we have reconstituted artificial peritoneal tissue which consists of mesothelial cell monolayer and connective tissue. We also established an in vivo model of artificial peritoneum using rat artificial peritoneum transplantation model. Our result may provide a new useful peritoneum model in which we can assess pathophysiology of many disease related to peritoneum. Light and elecron microscopy studies have confirmed this artificial peritoneal tissue closely resembled the morphological properties of in vivo tissue. Surface of this tissue was covered with mesothelial monolayer that was stained positive for cytokeratin CAM5.2. Although we have tried to transfect IFN-_γ gene-coding plasmid to the artificial peritoneum, no detectable amount of IFN-_γ have been found so far. For in vivo model, we have made artificial peritoneum from WKA rat and transplanted it to peritoneal defect of the syngenic rat. The transplanted peritoneum was well adapted to the surrounding peritoneum with minor response of host inflammatory cells and little adhesion. In the histological examination, the peritoneum had microvessel suggesting that the transplanted peritoneum induced angiogenesis. Collectively, our artificial peritoneum provide a novel experimental model of peritoneal disease such as malignant or infectious peritonitis. Furthermore our artificial peritoneal tissue may become a transplantable bio-engineered material for surgical peritoneal defect.

腹膜是生理上重要的组织，是腹膜腔与全身循环系统之间的屏障。在本研究中，我们重建了由间皮细胞单层和结缔组织组成的人工腹膜组织。采用大鼠人工腹膜移植模型，建立了人工腹膜在体模型。我们的研究结果可能提供一个新的有用的腹膜模型，我们可以评估许多疾病的病理生理与腹膜有关。光镜和电镜研究证实这种人工腹膜组织与体内组织的形态学特征非常相似。该组织的表面覆盖有细胞角蛋白CAM 5.2染色阳性的间皮细胞单层。虽然我们曾尝试将IFN-γ基因编码质粒转染到人工腹膜中，但至今未检测到IFN-γ的表达。在体内模型方面，我们采用WKA大鼠制作人工腹膜，并将其移植到同系大鼠的腹膜缺损处。移植腹膜与周围腹膜适应良好，宿主炎症细胞反应轻微，粘连少。组织学检查显示腹膜内有微血管生成，提示移植腹膜诱导了血管生成。总的来说，我们的人工腹膜提供了一种新的腹膜疾病，如恶性或感染性腹膜炎的实验模型。此外，我们的人工腹膜组织可能成为一种可移植的生物工程材料，用于外科腹膜缺损。