Artificial Peritoneum for Cancer Research and Surgical Treatment

腹膜是一种重要的生理组织，是腹膜与全身循环系统之间的屏障。在本研究中，我们重建了由间皮细胞单层和结缔组织组成的人造腹膜组织。我们还利用大鼠人工腹膜移植模型建立了体内人工腹膜模型。我们的结果可能提供一个新的有用的腹膜模型，在此模型中我们可以评估许多与腹膜相关的疾病的病理生理学。光学和电子显微镜研究证实，这种人造腹膜组织与活体组织的形态特征非常相似。组织表面覆盖有细胞角蛋白CAM5.2染色阳性的间皮单分子层。虽然我们已经尝试将干扰素-γ基因编码的质粒转入人工腹膜，但至今还没有发现可检测到的干扰素-γ。对于体内模型，我们制作了WKA大鼠的人工腹膜，并将其移植到同种异体大鼠的腹膜缺损处。移植腹膜与周围腹膜适应良好，宿主炎症细胞反应轻微，粘连少。在组织学检查中，腹膜有微血管形成，提示移植腹膜诱导了血管生成。总的来说，我们的人工腹膜提供了一种新的腹膜疾病的实验模型，如恶性腹膜炎或感染性腹膜炎。此外，我们的人工腹膜组织可能成为一种可移植的生物工程材料，用于外科腹膜缺损。

Peritoneum is an physiologically important tissue which serve as barrier between peritoneal cavity and general circulation system. In the present study, we have reconstituted artificial peritoneal tissue which consists of mesothelial cell monolayer and connective tissue. We also established an in vivo model of artificial peritoneum using rat artificial peritoneum transplantation model. Our result may provide a new useful peritoneum model in which we can assess pathophysiology of many disease related to peritoneum. Light and elecron microscopy studies have confirmed this artificial peritoneal tissue closely resembled the morphological properties of in vivo tissue. Surface of this tissue was covered with mesothelial monolayer that was stained positive for cytokeratin CAM5.2. Although we have tried to transfect IFN-_γ gene-coding plasmid to the artificial peritoneum, no detectable amount of IFN-_γ have been found so far. For in vivo model, we have made artificial peritoneum from WKA rat and transplanted it to peritoneal defect of the syngenic rat. The transplanted peritoneum was well adapted to the surrounding peritoneum with minor response of host inflammatory cells and little adhesion. In the histological examination, the peritoneum had microvessel suggesting that the transplanted peritoneum induced angiogenesis. Collectively, our artificial peritoneum provide a novel experimental model of peritoneal disease such as malignant or infectious peritonitis. Furthermore our artificial peritoneal tissue may become a transplantable bio-engineered material for surgical peritoneal defect.