Increased urinary excretion of bilirubin oxidative metabolites in septic patients: a new marker for ocidative stress in vivo

脓毒症患者尿中胆红素氧化代谢物排泄增加：体内氧化应激的新标志物

• 批准号: 12671234
• 负责人: SHIMIZU Shuji
• 金额: $ 2.37万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671234/
• 关键词: bilirubin; sepsis; oxidative stress; antioxidant; urine; severity; surgery; complication

Background. Bilirubin oxidative metabolites (BOMs) are generated from bilirubin as a result of its scavenging action against free radicals. During sepsis, excess amounts of free radicals are produced, and they play an important role in the pathophysiological process. We studied whether urinary excretion of BOMs would increase under septic conditions in humans and compared BOM levels with other well-established clinical parameters of inflammation.Methods. In 19 septic patients and 28 nonseptic control patients, the BOM concentrations in urine were measured by enzyme-linked immunosorbent assay with an anti-bilirubin antibody.Results. Urinary BOM levels in septic patients were much higher than those in control patients (21.6±2.5 v.s. 1.4±0.4 mol/g creatinine, P < 0.001). Although there was a linear correlation between urinary BOM and serum total bilirubin levels (r = 0.392, P = 0.008), serum bilirubin levels were not significantly higher in the septic group than in the control group (P = 0.072). BOM levels correlated with body temperature (r = 0.801, P < 0.001), white blood cell counts in the peripheral blood (r = 0.590, P < 0.001), serum C-reactive protein (r = 0.653, P < 0.001), and the acute physiological and chronic health evaluation (APACHE II) score (r = 0.467, P = 0.003).Conclusions. These results demonstrated a urinary increase in BOMs in septic patients. This increase indicates that urinary BOM level is a possible marker for continuous monitoring of sepsis severity in clinical practice.

背景胆红素氧化代谢产物（BOM）是胆红素清除自由基的结果。在脓毒症期间，产生过量的自由基，并且它们在病理生理过程中发挥重要作用。我们研究了在人类脓毒症条件下，BOM的尿排泄是否会增加，并将BOM水平与其他公认的炎症临床参数进行了比较。采用酶联免疫吸附试验（ELISA）检测19例脓毒症患者和28例非脓毒症对照者尿中BOM浓度。脓毒症患者尿BOM水平明显高于对照组（21.6±2.5 vs. 1.4± 0.4mol/g肌酐，P < 0.001）。虽然尿BOM与血清总胆红素水平呈线性相关（r = 0.392，P = 0.008），但脓毒症组的血清胆红素水平并不显著高于对照组（P = 0.072）。与体温相关的BOM水平（r = 0.801，P < 0.001），外周血白色细胞计数（r = 0.590，P < 0.001），血清C反应蛋白急性生理和慢性健康状况评分（APACHE II）评分（r = 0.467，P = 0.003）。这些结果表明脓毒症患者的尿液BOM增加。这一增加表明尿BOM水平是临床实践中持续监测脓毒症严重程度的可能标志物。

项目成果

Kazahiro Otani, Shuji Shimizu, Kazuo Chijiiwa, et al.: "Increased urinary excretion of bilirubin oxidative metabolites in septic patients : a new marker for oxidative stress"Journal of Surgical Research. 96. 44-49 (2001)

Kazahiro Otani、Shuji Shimizu、Kazuo Chijiiwa 等人：“脓毒症患者胆红素氧化代谢物尿排泄增加：氧化应激的新标志物”《外科研究杂志》。

Kazuhiro Otani, Shuji Shimizu, Kazuo Chijiiwa, et al.: "Increased urinary excretion of bilirubin oxidative metabolites in septic patients: a new marker for oxidative stress"Journal of Surgical Research. 96. 44-49 (2001)

Kazuhiro Otani、Shuji Shimizu、Kazuo Chijiiwa 等人：“脓毒症患者胆红素氧化代谢物尿排泄增加：氧化应激的新标志物”《外科研究杂志》。

Kazuhiro Otani, Shuji Shimizu, Kazuo Chijiiwa, et al.: "Increased urinary excretion of bilirubin oxidative metabolites in septic patients : a new marker for oxidative stress"Journal of Surgical Research. 96. 44-49 (2001)

Kazuhiro Otani、Shuji Shimizu、Kazuo Chijiiwa 等人：“脓毒症患者胆红素氧化代谢物尿排泄增加：氧化应激的新标志物”《外科研究杂志》。

Kazuhiro Otani,Shuji Shimizu,Kazuo Chijiiwa,Koji Yamaguchi,Masao Tanaka: "Increased urinary excretion of bilirubin oxidative metabolites in septic patients : a new marker for oxidative stress"Journal of Surgical Research. (印刷中). (2001)

Kazuhiro Otani、Shuji Shimizu、Kazuo Chijiiwa、Koji Yamaguchi、Masao Tanaka：“脓毒症患者胆红素氧化代谢物尿排泄增加：氧化应激的新标志”《外科研究杂志》（2001 年）。