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The induction of heme oxygenese-1 in intestinal mucosa of septic rat

The induction of heme oxygenese-1 in intestinal mucosa of septic rat
脓毒症大鼠肠黏膜血红素氧合酶1的诱导
批准号:
09671234
负责人:
SHIMIZU Shuji
金额:
$1.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1997
资助国家:
日本
项目状态:
已结题
起止时间:
1997 至 1998

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英文摘要
Backgrounds : Bilirubin is known to be a physiological antioxidant. Heme oxygenase (HO)-1, the rate-limiting enzyme in the heme degradation, is known to be induced by oxidative stress in the liver, kidney, or brain, but its induction has not yet been known in the intestine, We studied the expression of HO-1 in intestinal mucosa induced by oxidative stress using a rat septic model. Methods : Male Wistar rats were given with lipopolysaccharide (LPS) from E.coli (10mg/kg/body weight) by peritoneal injection. They were killed at 0, 1, 3, 5, 7.5, 10 and 24 hrs and their small intestinal mucosa was harvested. In intestinal mucosa, reduced glutathione (GSH), lipid peroxide, HO-1 mRNA, HO-1 protein, bilirubin, and bilirubin oxidative metabolites (BOM) were measured. Results : Intestinal GSH level was significantly decreased after 3 to 5 hrs by 40% of the control level. Lipid peroxides level was 1 .7-folds of control level at 5 hrs. The level of HO-1 mRNA increased to 3 times of the control at 3 hrs and HO-1 protein was strongly detected 7.5 hrs after LPS injection. Bilirubin level increased to 4-folds of control (peak level) at 10 hrs and BOM showed 5-folds increase with the peak level at 10 hrs. Conclusions : Sepsis reduced the GSH content and increased lipid peroxides. This intestinal oxidative stress induced the expression of HO-1 mRNA as well as the production of the protein in the intestinal mucosa. Bilirubin production and subsequent oxidation to BOM indicates that bilirubin acted as an antioxidant. This suggested that small intestine had ability to respond quickly to oxidative stress in sepsis, not just as a digestive and absorptive organ.
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  • 批准号:
    20406027
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $10.9万
  • 财政年份:
    2008
  • 负责人:
    SHIMIZU Shuji
  • 依托单位:
Effect of hyperbaric oxygen on hyperbiliri.ibinemia from the viewpoint of antioxidant activity of bilirubin
  • 批准号:
    14571209
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.18万
  • 财政年份:
    2002
  • 负责人:
    SHIMIZU Shuji
  • 依托单位:
海外基金