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Prevalence and Molecular Biological Feature of Human Aberrant Crypt Foci

人类异常隐窝病灶的患病率和分子生物学特征

基本信息

批准号：
12671255
负责人：
TOGASHI Kazutomo
金额：
$ 2.11万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671255/
关键词：
aberrant crypt foci K ras adenoma hyperplastic polyp colonoscopy aberrant crypt foci K-ras 大腸腺種鋸歯状腺種拡大内視鏡大腸癌

项目摘要

Purpose : To study the nature of human aberrant crypt foci (ACF). Methods: Lower part of rectums in 166 patients who gave informed consent were observed by magnifying chromo-colonoscopy for identifying and counting ACF. 125 biopsy specimens proved to be histologically ACF and were analyzed the sequence of K-ras, exon 1 by using nested PCR and direct sequencing. The sequences of K-ras in 52 hyperplastic polyps were analyzed as a comparison. Results : Median number of ACF counted in patients who had undergone colorectal cancer (CRC) surgery or who had CRC at the time of colonoscopy was the same as that in patients without CRC. Median number of ACF had no significant difference regarding gender, but that of ACF in patients with the age of 70-year or older was greater than that in younger patients (4 versus 2, p<0.0001,). Histologic diagnoses of 125 biopsy specimens were dysplastic ACF in 11, hyperplastic ACF in 26 and non-hyperplastic ACF in 88. Dysplastic ACF can be clearly discriminated from the other histologic types of ACFs, but there were a small number of intermediate type ACFs between hyperplastic ACF and non-hyperplastic ACF.K-ras mutation rates are shown in the table. K-ras mutation rate in hyperplastic ACF is similar to that in hyperplastic polyp. Non-hyperplastic ACF as well as dysplastic ACF showed higher mutation rate than hyperplastic ACF, but K-ras mutantation type was different between non-hyperplastic ACF and dysplastic ACF. Conclusions: Hyperplastic ACF can be regarded as a precursor of hyperplastic polyp. Analysis of K-ras mutant type may suggest that non-hyperplastic ACF and hyperplastic ACF are different from dysplastic ACF.

目的：研究人类异常隐窝病灶（ACF）的性质。方法：对166例知情同意的患者，采用放大染色结肠镜观察直肠下段ACF的识别和计数。125例活检标本经组织学证实为ACF，采用巢式PCR和直接测序法分析K-ras外显子1的序列。对52例增生性息肉的K-ras基因序列进行分析比较。结果：结直肠癌（CRC）手术患者或结肠镜检查时结直肠癌患者的ACF中位数与未结直肠癌患者相同。ACF中位数在性别上无显著差异，但70岁及以上患者的ACF中位数大于年轻患者（4比2，p<0.0001,）。125例活检标本的组织学诊断为ACF发育不良11例，增生ACF 26例，非增生ACF 88例。发育不良ACF与其他组织学类型的ACF有明显区别，但在增生性ACF与非增生性ACF之间存在少量中间型ACF。K-ras突变率见表。增生性ACF中K-ras突变率与增生性息肉相似。非增生性ACF和发育不良ACF的突变率均高于增生性ACF，但K-ras突变类型在非增生性ACF和发育不良ACF之间存在差异。结论：增生性ACF可视为增生性息肉的前兆。分析K-ras突变型可能提示非增生性ACF和增生性ACF与发育不良ACF不同。