Analysis of microcirculation of experimental metastatic lung tumor using artificial oxygen carrier as a tool for altering the microvascular tone

使用人工氧载体作为改变微血管张力的工具分析实验性转移性肺肿瘤的微循环

基本信息

  • 批准号:
    12671328
  • 负责人:
  • 金额:
    $ 1.54万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

The environment of metastatic tumor in the lung is much different in terms of oxygen supply and microcirculation compare to those metastatic tumors in other organs. To investigate the establishment of microcirculation of metastatic lung tumor, we have to establish syngenic model of lung metastasis.To observe lung microcirculation, we implanted a crystal window chamber in the chest wall of a rat. Through this window, we could observe pulmonary arteriole, alveolar capillary, and pulmonary vein using laser confocal microscope and fluorescent dye. The images were recorded in VCR and analyzed afterwards. Inner diameter of vasculature could be measured and blood flow could be evaluated in capillary area. More sophistication was necessary to measure the blood flow in pulmonary arteriole because flow was fast enough to capture individual fluorescent labeled red blood cells. To establish lung metastasis, we evaluated 5 models using LY80 which is ascitis hepatoma in Donryu rat. Examined modaliti … More es were as follows, intraperitoneal tumor cell injection, subcutaneous implantation of tumor cells, implantation of tumor cells to the lung directly by thoracotomy, tail vein infusion of tumor cells, and tail vein infusion of small tissue suspension taken from subcutaneous implanted tumor. Among these modalities, tail vein infusion of small tissue suspension taken form subcutaneous implanted tumor gave us the most reliable and stable results. After 15 to 17 days after infusion, we could evaluate the pulmonary metastatic tumor.To evaluate the reactivity of tumor microvasculature, we used oxyhemoglobin solution and HbV suspension. Oxyhemoglobin solution is a strong potential of contraction of microvasculature, while HbV (liposome encapsulated hemoglobin), which is originally developed as an artificial oxygen carrier, is thought to have little effect on microvasculature. In normal animals, pulmonary arterioles contract after injection of Oxyhemoglobin while transient contraction was observed after HbV injection. There are little change of diameter, flow in alveolar capillary. However, pulmonary arteriole react these challenges revealing that Nitric Oxide is one of major modulator in determining the tone of pulmonary vasculature.In tumor burden animals, especially in current model, alveolar capillary was dilated. But we could not observe any changes in arteriole and/or venule area. The effect of oxyhemoglobin and HbV in this model is similar to those observed in intact animals. We have to improve the model to analyze the event in microcirculation of metastatic lung tumor. Less
肺转移瘤的氧供和微循环环境与其他器官转移瘤有很大不同。为了研究肺转移瘤微循环的建立,必须建立同系肺转移瘤模型,为了观察肺微循环,我们在大鼠胸壁植入了一个晶体窗小室。通过这个窗口,我们可以用激光共聚焦显微镜和荧光染料观察肺小动脉、肺泡毛细血管和肺静脉。将图像记录在VCR中并随后进行分析。可以测量血管的内径,并可以评估毛细血管区域的血流。需要更复杂的方法来测量肺小动脉中的血流,因为血流速度足够快,可以捕获单个荧光标记的红细胞。为了建立肺转移模型,我们用LY 80腹水肝癌在东柳大鼠进行了5种模型的评价。检查模态 ...更多信息 方法:腹腔内肿瘤细胞注射、皮下肿瘤细胞植入、开胸直接肺内肿瘤细胞植入、尾静脉输注肿瘤细胞、尾静脉输注皮下植入肿瘤的小组织悬液。在这些方法中,尾静脉输注来自皮下植入肿瘤的小组织悬液给我们提供了最可靠和稳定的结果。输注后15 ~ 17天,我们可以评估肺转移瘤,为了评估肿瘤微血管的反应性,我们使用氧合血红蛋白溶液和HbV悬液。氧合血红蛋白溶液具有很强的收缩微血管的潜力,而最初作为人工氧载体开发的HbV(脂质体包裹的血红蛋白)被认为对微血管几乎没有影响。在正常动物中,肺小动脉在注射氧合血红蛋白后收缩,而在注射HbV后观察到短暂收缩。肺泡毛细血管直径、流量变化不大。然而,肺小动脉对这些挑战作出反应,表明一氧化氮是决定肺血管张力的主要调节剂之一。在肿瘤负荷动物中,特别是在本模型中,肺泡毛细血管扩张。但未观察到微动脉和/或微静脉面积的变化。该模型中氧合血红蛋白和HbV的作用与在完整动物中观察到的作用相似。为了更好地分析肺转移瘤的微循环事件,需要对模型进行改进。少

项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Iwamaru A, Watanabe M, Yu S, Ohtsuka T, Horinouchi H, Kobayashi K: "Measurement of tumor blood flow using dye extraction microspheres in two rat tumor models"Int J Oncol. 18. 227-232 (2001)
Iwamaru A、Watanabe M、Yu S、Ohtsuka T、Horinouchi H、Kobayashi K:“在两种大鼠肿瘤模型中使用染料提取微球测量肿瘤血流”Int J Oncol。
  • DOI:
  • 发表时间:
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Iwamaru A, Yu S, Ohtsuka T, Watanabe M, Horinouchi H, Kobayahi: "Measurement of tumor blood flow using dye extraction microspheres in two rat tumor models"Int J Oncol. 18. 227-232 (2001)
Iwamaru A、Yu S、Ohtsuka T、Watanabe M、Horinouchi H、Kobayahi:“在两种大鼠肿瘤模型中使用染料提取微球测量肿瘤血流”Int J Oncol。
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    0
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HORINOUCHI Hirohisa其他文献

HORINOUCHI Hirohisa的其他文献

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{{ truncateString('HORINOUCHI Hirohisa', 18)}}的其他基金

Analysis of oxygen transport to malignant tumor and mechanism of enhancing effect of treatment by oxygenation of tumor tissue
恶性肿瘤氧输送及肿瘤组织氧合增强治疗效果的机制分析
  • 批准号:
    21591824
  • 财政年份:
    2009
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Enhanced radiation response andn inhibition of angiogenesis by artificial oxygen carrier
人工氧载体增强辐射反应并抑制血管生成
  • 批准号:
    18591560
  • 财政年份:
    2006
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Control of angiogenesis by attenuation of oxygen tension in the metastatic tumor of the lung
通过减弱肺转移性肿瘤中的氧张力来控制血管生成
  • 批准号:
    16591410
  • 财政年份:
    2004
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of metastasis mechanism in experimental lung metastasis with heparanase transgenic tumor cells
乙酰肝素酶转基因肿瘤细胞实验性肺转移机制分析
  • 批准号:
    14571278
  • 财政年份:
    2002
  • 资助金额:
    $ 1.54万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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Evaluation and therapeutic application of lymphangiogenesis using animal model with lymph node metastasis of lung neoplasm
肺肿瘤淋巴结转移动物模型淋巴管生成的评价及治疗应用
  • 批准号:
    22591561
  • 财政年份:
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  • 批准号:
    15591487
  • 财政年份:
    2003
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  • 项目类别:
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COMPARISON OF SPONTANEOUS & OZONE-INDUCED LUNG NEOPLASM IN B6C3F1 MICE
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  • 批准号:
    3755392
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