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The morphological and functional change of the gap junction and the effect of irsogladine maleate in the peripheral nerve regeneration

间隙连接的形态和功能变化及马来酸伊索拉定在周围神经再生中的作用

基本信息

批准号：
12671427
负责人：
KITAMURA Hirokazu
金额：
$ 2.18万
依托单位：
OITA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The research in this fiscal year did next fact that "The morphological analysis of the Cx32 expression in rat normal peripheral nerve." by continueing in last year. It is already reported that Cx32 signal was recognized at the myelin sheath of peripheral nerve near the node of Ranvier by the fluorescence microscope. In this fiscal year, the peripheral nerve was observed by transmission electron microscope comparison to the immunoelectron microscopy image using the HAM8 antibody which recognized Cx32 was carried out.In the observation of the super micro localization of the gap junction in normal peripheral nerve, the artifact of the vortex state was seen in the myelin sheath even if using the various fixative solution, embedding agent, and temperature etc. And, It also continues on the change of the Cx32 expression in orthograde degeneration provocation peripheral nerve at present, the examination is being carried out.In the meantime, support of this study subsidy, the research of the C … More x32 expression in the lachrymal gland organization was also carried out beingly which Cx32 equal to the peripheral nerve has manifested the gland tissue. Immunofluorescent stain and immunoelectron microscopy image using the HAM8 antibody were observed on rat lachrymal gland tissue slice and primary cultured cells of the gland, and the localization of the gap junction was clarified. It was revealed that two types of gap junctions existed at rat lacrymal gland tissue in vivo. One was the intercellular type between adjacencies gland cell and the other was reflexive type of gap junction between self cell membrane. Later type was clarified where it had been formed in basal plane. In the experimental result of in vitro using the HAM8 antibody, the time-dependent transfer of the gap junction spot was clarified. By the and, transmission electron microscope, they were revealed that the reconstruction cell aggregate had the structure which is morphologically differentiated and intercellular1 gap junction existed between adjacent lacrymal gland cells. It was morphologically shown that this was functionally committed even in the reconstruction lachrymal gland cell.The subsidy was put in consumable stores, etc. without purchasing image recording personal computer set applied in the relation with the delivery amount in the purchase schedule as this fiscal year furniture. Less

本财年的研究接下来进行了“大鼠正常周围神经中Cx32表达的形态学分析。"去年继续。荧光显微镜观察到在Ranvier结附近的周围神经髓鞘中有Cx32信号。在本财政年度，通过透射电子显微镜观察周围神经，与使用识别Cx32的HAM 8抗体的免疫电子显微镜图像进行比较。在正常周围神经中差距连接的超微定位观察中，即使使用各种固定液、包埋剂、另外，目前还在继续对Cx32在顺行性变性诱发周围神经中表达的变化进行检测，同时，在本研究资助下， ...更多信息 x32在泪腺组织中也有表达，其中Cx32相当于周围神经表现了腺体组织。用HAM 8抗体对大鼠泪腺组织切片和原代培养的泪腺细胞进行免疫荧光染色和免疫电镜观察，明确了差距连接的定位。结果表明，在体大鼠泪腺组织中存在两种类型的缝隙连接。一种是相邻腺细胞间的细胞间型，另一种是自身细胞膜间的反射型缝隙连接。后来的类型是明确的，它已经形成在基面。在使用HAM 8抗体的体外实验结果中，明确了差距连接点的时间依赖性转移。透射电镜观察显示，重建的泪腺细胞团具有形态分化的结构，相邻泪腺细胞间存在细胞间隙连接。从形态学上看，即使在再造泪腺细胞中，这也是功能性的。补贴放在消费品商店等，而不购买图像记录个人计算机组，作为本财政年度的家具，与采购计划中的交付量有关。少