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The morphological and functional change of the gap junction and the effect of irsogladine maleate in the peripheral nerve regeneration

间隙连接的形态和功能变化及马来酸伊索拉定在周围神经再生中的作用

基本信息

批准号：
12671427
负责人：
KITAMURA Hirokazu
金额：
$ 2.18万
依托单位：
OITA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The research in this fiscal year did next fact that "The morphological analysis of the Cx32 expression in rat normal peripheral nerve." by continueing in last year. It is already reported that Cx32 signal was recognized at the myelin sheath of peripheral nerve near the node of Ranvier by the fluorescence microscope. In this fiscal year, the peripheral nerve was observed by transmission electron microscope comparison to the immunoelectron microscopy image using the HAM8 antibody which recognized Cx32 was carried out.In the observation of the super micro localization of the gap junction in normal peripheral nerve, the artifact of the vortex state was seen in the myelin sheath even if using the various fixative solution, embedding agent, and temperature etc. And, It also continues on the change of the Cx32 expression in orthograde degeneration provocation peripheral nerve at present, the examination is being carried out.In the meantime, support of this study subsidy, the research of the C … More x32 expression in the lachrymal gland organization was also carried out beingly which Cx32 equal to the peripheral nerve has manifested the gland tissue. Immunofluorescent stain and immunoelectron microscopy image using the HAM8 antibody were observed on rat lachrymal gland tissue slice and primary cultured cells of the gland, and the localization of the gap junction was clarified. It was revealed that two types of gap junctions existed at rat lacrymal gland tissue in vivo. One was the intercellular type between adjacencies gland cell and the other was reflexive type of gap junction between self cell membrane. Later type was clarified where it had been formed in basal plane. In the experimental result of in vitro using the HAM8 antibody, the time-dependent transfer of the gap junction spot was clarified. By the and, transmission electron microscope, they were revealed that the reconstruction cell aggregate had the structure which is morphologically differentiated and intercellular1 gap junction existed between adjacent lacrymal gland cells. It was morphologically shown that this was functionally committed even in the reconstruction lachrymal gland cell.The subsidy was put in consumable stores, etc. without purchasing image recording personal computer set applied in the relation with the delivery amount in the purchase schedule as this fiscal year furniture. Less

本财年的研究做了“大鼠正常周围神经中Cx32表达的形态学分析”。继续去年。据报道，荧光显微镜在靠近Ranvier结的周围神经髓鞘处识别到了Cx32信号。本财年，通过透射电子显微镜观察周围神经，与使用识别Cx32的HAM8抗体的免疫电子显微镜图像进行比较。在正常周围神经间隙连接的超微定位观察中，即使使用各种固定液、包埋剂和温度，在髓鞘中也可以看到涡旋状态的伪影。并且，目前还继续对顺行变性激发周围神经中Cx32表达的变化进行检测。同时，在本研究资助的支持下，还进行了泪腺组织中Cx32表达的研究，即Cx32相当于周围神经在腺组织中的表达。对大鼠泪腺组织切片和腺体原代培养细胞进行免疫荧光染色和使用HAM8抗体的免疫电镜观察，明确了间隙连接的定位。研究表明，体内大鼠泪腺组织中存在两种类型的间隙连接。一种是相邻腺细胞之间的细胞间连接型，另一种是自身细胞膜之间间隙连接的反射型。后来的类型被澄清是在基面中形成的。在体外使用HAM8抗体的实验结果中，阐明了间隙连接点的时间依赖性转移。透射电镜观察显示，重建细胞聚集体具有形态分化的结构，相邻泪腺细胞之间存在细胞间间隙连接。从形态上看，即使在重建泪腺细胞中，这也在功能上发生。补贴被投入到消耗品商店等，而不是购买与本财政年度家具的采购计划中的交货量相关的图像记录个人电脑套件。较少的