Monitoring of brain oxygen status using near-infrared spectroscopy

使用近红外光谱监测脑氧状态

• 批准号: 12671486
• 负责人: KAKIHANA Yasuyuki
• 金额: $ 2.24万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671486/
• 关键词: Near-infrared spectroscopy; cytochrome oxidase; deep hypothermic circulatory arrest; Cardiopulmonary bypass; cerebral oxygenation; 近赤外分光法; チトクロームオキシターゼ; 超低体温; 完全循環停止; 脳酸素化状態; S-100b蛋白; 内頸静脈酸素飽和度; チトフロームオキシターゼ; S-100蛋白

Although the signal obtained for cytochrome oxydase (cyt. ox.) by near-infrared spectroscopy (NIRS) should be a useful indicator of intracellular hypoxia, the cyt. ox. signal represents a small component of the total change, and is therefore vulnerable to artifact. Recently it has been reported that cyt. ox. signal measured by NIRS is highly dependent on hematocrit. We have developed a new approach to the measurement of cyt. ox. in the brain involving the use of our new algorithm. In this study, we used NIRS device with our algorithm to examine possible cross-talk between the cytochrome and Hb signals while a change was occurring in the hemodilution. In another experiments, deep hypothermic circulatory arrest (DHCA) was induced for 60 min under 25℃ or 15℃ and evaluated the relationship between the redox behavior of cyt. ox. during DHCA and postoperative neurological prognosis. After institution of cardiopulmonary bypass (CPB), dogs received sodium cyanide to uncouple cytochrome from Hb. The hematocrit was then decreased from 35 to 5% by hemodilution. In this study, the Hb concentration calculated by NIRS device showed a strong positive correlation with hematocrit values in the blood during hemodilution. However, there was no correlation between the hematocrit and the cyt. ox. signal, which indicate that the cyt. ox. signal calculated using our recently developed algorithm was not much influenced by hematocrit. Furthermore, there was a good correlation between the redox behavior of cyt. ox. in the brain during DHCA and postoperative brain injury. In conclusion, the cyt. ox. signal measured by NIRS is dependent on the algorithm employed. Therefore, provided an accurate algorithm is employed the cyt. ox. signal measured by NIRS should provide us with important information even under various changes in hematocrit and/or hypothermia.

虽然通过近红外光谱 (NIRS) 获得的细胞色素氧化酶 (cyt. ox.) 信号应该是细胞内缺氧的有用指标，但细胞色素氧化酶 (cyt. ox.) 信号应该是细胞内缺氧的有用指标。牛。信号代表总变化的一小部分，因此容易受到伪影的影响。最近有报道称，cyt.牛。 NIRS 测量的信号高度依赖于血细胞比容。我们开发了一种测量细胞色素的新方法。牛。在大脑中涉及使用我们的新算法。在这项研究中，我们使用 NIRS 设备和我们的算法来检查血液稀释发生变化时细胞色素和 Hb 信号之间可能存在的串扰。在另一项实验中，在25℃或15℃下诱导深低温停循环（DHCA）60分钟，并评估细胞色素氧化还原行为之间的关系。牛。 DHCA 期间和术后神经系统预后。进行体外循环 (CPB) 后，狗接受氰化钠以将细胞色素与血红蛋白解偶联。然后通过血液稀释将血细胞比容从 35% 降低至 5%。本研究中，NIRS装置计算出的Hb浓度与血液稀释过程中血液中的血细胞比容值呈强正相关。然而，血细胞比容和细胞色素之间没有相关性。牛。信号，表明细胞色素。牛。使用我们最近开发的算法计算的信号不受血细胞比容影响太大。此外，细胞色素的氧化还原行为之间存在良好的相关性。牛。 DHCA 期间和脑损伤术后的大脑中。总之，细胞色素。牛。 NIRS 测量的信号取决于所采用的算法。因此，只要采用准确的算法，细胞色素即可。牛。即使在血细胞比容和/或体温过低的各种变化下，NIRS 测量的信号也应该为我们提供重要信息。

项目成果

Yasuyuki Kakihana, Shunichiro Yamaguchi, Takeshi Omae, Tamotsu Kuniyoshi, Masanori Onomoto, Kazumi Tobo, Yoshitami Kadota, Kouichi Kawasaki, Yuichi Kanmura: "Cognitive dysfunction and cerebral monitoring after cardiopulmonary bypass - Significance of cyto

Yasuyuki Kakihana、Shunichiro Yamaguchi、Takeshi Omae、Tamotsu Kuniyoshi、Masanori Onomoto、Kazumi Tobo、Yoshitami Kadota、Kouichi Kawasaki、Yuichi Kanmura：“体外循环后的认知功能障碍和脑监测 - 细胞的意义

Yasuyuki Kakihana, Akira Matsunaga, Kazumi Tobo, Sukazu Isowaki, Masayuki Kawakami, Isao Tsuneyoshi, Yuichi Kanmura, Mamoru Tamura: "Redox behavior of cytochrome oxidase and neurological prognosis in 66 patients who underwent thoracic aortic surgery"Europ

Yasuyuki Kakihana、Akira Matsunaga、Kazumi Tobo、Sukazu Isowaki、Masayuki Kawakami、Isao Tsuneyoshi、Yuichi Kanmura、Mamoru Tamura：“66 例接受胸主动脉手术的患者的细胞色素氧化酶的氧化还原行为和神经学预后”Europ

Yasuyuki Kakihana, Akira Matsunaga, Kazumi Tobo, Sumikazu Isowaki, Masayuki Kawakami, Isao Tsuneyoshi, Yuichi Kanmura, Mamoru Tamura: "Redox behavior of cytochrome oxidase and neurological prognosis in 66 patients who underwent thoracic aortic surgery"Eur

Yasuyuki Kakihana、Akira Matsunaga、Kazumi Tobo、Sumikazu Isowaki、Masayuki Kawakami、Isao Tsuneyoshi、Yuichi Kanmura、Mamoru Tamura：“66 名接受胸主动脉手术的患者的细胞色素氧化酶的氧化还原行为和神经学预后”Eur

垣花泰之, 山口俊一郎, 尾前 毅, 国吉 保, 尾野本真徳, 富房和巳, 門田善民, 川崎孝一, 上村裕一: "体外循環に起因する脳高次機能と脳モニタリング-近赤外線分光法(NIRS)によるcytochrome oxidase測定の意義-"臨床麻酔. 25・12. 1881-1885 (2001)

Yasuyuki Kakihana、Shunichiro Yamaguchi、Takeshi Omae、Tamotsu Kuniyoshi、Masanori Onomoto、Kazumi Tomifusa、Yoshitami Kadota、Koichi Kawasaki、Yuichi Uemura：“体外循环引起的高级脑功能和脑监测 - 近红外光谱通过（ NIRS）-“临床麻醉。25・12。1881-1885（2001）

垣花泰之, 国吉 保, 磯脇純和, 永田悦郎, 川上雅之, 垣吉勇雄, 上村裕一, 田村 守: "近赤外線分光法によるミトコンドリア内チトクロームオキシダーゼ測定について"脈管学. 42. 6-9 (2002)

Yasuyuki Kakihana、Tamotsu Kuniyoshi、Sumi-kazu Isowaki、Etsuro Nagata、Masayuki Kawakami、Isao Kakiyoshi、Yuichi Uemura、Mamoru Tamura：“通过近红外光谱法测量线粒体内细胞色素氧化酶”血管学42. 6-9 (2002))。