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Regulation of Prolactin Secretion from Pituitary Lactotrophs in the Presence of Activin

激活素存在下垂体催乳素分泌的调节

基本信息

批准号：
12671577
负责人：
ABE Yumiko
金额：
$ 0.9万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671577/
关键词：
Activin Follistatin Inhibin Prolactin Growth Hormone Pituitary

项目摘要

It has been reported that activin suppressed the synthesis and secretion of prolactin (PRL) and growth hormone (GH) using the primary anterior pituitary cell culture or cell lines derived from somatotrophs or lactotrophs. Recently, it was also reported that activin suppressed the synthesis of PRL but stimulated the synthesis of GH in GH3 cells derived from rat somatolactotroph. The culture media used in all of these experiments contained sera. Therefore, it was speculated that the growth factors and cytokines in sera interacted with activin in the above culture systems. Antibiotics were also present. Aminoglidosids are the antibiotics often used in cell culture and it was reported that aminoglycosides suppressed the hydrolysis of phosphoinositide. Therefore, not only growth factors and cytokines in sera but also antibiotics might affect the signal transduction pathways of activin. In order to study the effect of activin on the synthesis of PRL and GH without other factors interfering, we established the culture system of GH3 cells using a defined medium without the addition of sera or antibiotics. We found that activin stimulated the synthesis of both PRL and GH in this system. Time dependent and dose dependent stimulation was observed. The activin binding protein follistatin suppressed the increased synthesis of PRL and GH in the presence of activin in a dose dependent manner. We also found that MAP kinase inhibitors inhibited the increased synthesis of PRL and GH in the presence of activin. Activin may regulate the synthesis of PRL and GH by interacting with MAP kinase pathways.

据报道，使用原代垂体前叶细胞培养物或源自生长激素细胞或催乳素细胞的细胞系，激活素可抑制催乳素（PRL）和生长激素（GH）的合成和分泌。最近，也有报道称，在大鼠生长泌乳素细胞来源的GH3细胞中，激活素抑制PRL的合成，但刺激GH的合成。所有这些实验中使用的培养基都含有血清。因此，推测血清中的生长因子和细胞因子与上述培养系统中的激活素相互作用。还存在抗生素。氨基糖苷类是细胞培养中常用的抗生素，据报道氨基糖苷类可抑制磷酸肌醇的水解。因此，不仅血清中的生长因子和细胞因子，抗生素也可能影响激活素的信号转导途径。为了在不受其他因素干扰的情况下研究激活素对PRL和GH合成的影响，我们使用确定的培养基建立了GH3细胞的培养体系，不添加血清或抗生素。我们发现激活素刺激该系统中 PRL 和 GH 的合成。观察到时间依赖性和剂量依赖性刺激。在存在激活素的情况下，激活素结合蛋白卵泡抑素以剂量依赖性方式抑制PRL和GH合成的增加。我们还发现 MAP 激酶抑制剂在激活素存在的情况下抑制 PRL 和 GH 合成的增加。激活素可通过与 MAP 激酶途径相互作用来调节 PRL 和 GH 的合成。