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A Comprehensive Study of the Inhibin/Activin-Family Proteins in Chorioamnionitis

绒毛膜羊膜炎中抑制素/激活素家族蛋白的综合研究

基本信息

批准号：
18591794
负责人：
ABE Yumiko
金额：
$ 1.25万
依托单位：
Gunma University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591794/
关键词：
chorioamnionitis inhibin activin dioxin comprehensive analysis microarray quantitative PCR real-time PCR

项目摘要

Activins are dimeric proteins composed of inhibin/activin beta-subunits, and their biological activities are regulated by inhibins, and activinbinding proteins called follistatins. Recently, the involvement of activins in inflammation and wound healing has been reported. In order to study the role of inhibin/activin family proteins in chorioamnionitis, which is the primary cause of premature rupture of the membranes, we used human amniotic cell culture with the permission of The Internal Review Board and the informed consent of the patients. We studied gene expressions in amniotic epithelial cells by DNA microarray and quantitative PCR analyses, and detected interferon-inducible genes and genes related to collagen synthesis or degradation as dioxin-inducible genes. DNA microarray analysis showed increased expression of the inhibin/activin beta A subunit also. The involvement of activins in the pathology of membranes by dioxins was suggested. In order to study if activins were involved in the inflammation in the compact layer and the fibroblast layer of the membranes, amniotic mesenchymal cells were studied. Tumor necrosis factor-alpha (TNF-alpha) did not increase the inhibin/activin beta B subunit mRNA but did increase the inhibin/activin beta A subunit mRNA. The increase of activin A protein was also shown by enzyme linked immunosorbent assay. Inflammatory cytokine TNF-alpha seemed to stimulate the production of activin A at both mRNA and protein levels in these cells.

激活素是由抑制素/激活素β亚基组成的二聚体蛋白，它们的生物活性由抑制素和激活素结合蛋白卵泡抑素调节。最近，活化素参与炎症和伤口愈合已被报道。为了研究抑制素/激活素家族蛋白在绒毛膜羊膜炎中的作用，这是导致膜早破的主要原因，我们在获得内部审查委员会许可和患者知情同意的情况下，使用了人羊膜细胞培养。我们通过DNA芯片和定量PCR分析羊膜上皮细胞的基因表达，检测干扰素诱导基因和胶原合成或降解相关基因为二恶英诱导基因。DNA微阵列分析显示抑制素/激活素β A亚基的表达也有所增加。激活素参与了二恶英对细胞膜的病理作用。为了研究激活素是否参与了羊膜致密层和成纤维层的炎症反应，我们对羊膜间充质细胞进行了研究。肿瘤坏死因子α (tnf - α)不增加抑制素/激活素β B亚基mRNA，但增加抑制素/激活素β A亚基mRNA。酶联免疫吸附试验也显示激活素A蛋白升高。炎症细胞因子tnf - α似乎在这些细胞的mRNA和蛋白水平上刺激激活素A的产生。