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Study on the role of inhibin-action system in the phisiology and pathophysiology of follicular growth

抑制素作用系统在卵泡生长的生理学和病理生理学中的作用研究

基本信息

批准号：
12671579
负责人：
FUJIWARA Toshihiro
金额：
$ 1.98万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

1.The expression levels of inhibin, activin, and follistatin mRNA were measured in granulose cells (GCs) obtained from IVF-ET patients, which were consequently evaluated if there was any relationship with several clinical parameters. As a result inhibin a-subunit mR.NA level was higher in follicles which contained maturing oocytes. However it was lower in follicles from which high quality embryos were obtained. This suggests though expression of inhibin a=subunit is necessary for oocyte maturation, its too much expression causes negative effect on embryos.2.Follicles of either normal cycling women or polycystic ovarian syndrome (PCOS) patients were transvaginally aspirated and both follicular fluid (FF) and GCs were obtained. Protein levels of inhibin (A or B), activin A, free follistatin and several steroid hormones (androstenedione, estradiol, rogesterone) in FF as well as niR.NA levels of inhibin subunits (a, (3A, (3B), follistatin subtypes (288 and 315) were measured. In normal cycling women androstenedione was high, while estradiol was low in FF of small follicles. As follicles grew, the fonner decreased, while the latter increased. Both itt ibin A and progesterone showed increase following. follicular growth, whereas inhibin B, activin A, free follistatin levels did not change significantly. In the levels of rnRNA, both inhibin a and (3A-subunit showed increase along with follicular growth, whereas neither inhibin (3B-subunit nor follistatin 288/315 changed significantly. In PCOS GCs ni NA levels of both inhibin subunit-a and (3A were significantly lower compared to those of size matched follicles. The other mRNA did not show significant difference. These findings imply the possible involvement of inhibin/activtn system in pathophysiology of PCOS.

1.测量了从IVF-ET患者中获得的颗粒细胞（GC）中抑制素、激活素和卵泡抑制素mRNA的表达水平，从而评估其是否与多个临床参数有任何关系。结果表明，含有成熟卵母细胞的卵泡中卵泡素a亚基mRNA水平较高。但在获得高质量胚胎的卵泡中，其含量较低。2.经阴道抽取正常月经周期妇女和多囊卵巢综合征（PCOS）患者的卵泡，采集卵泡液（FF）和GCs。检测FF中卵泡抑素（A或B）、激活素A、游离卵泡抑素和几种类固醇激素（雄烯二酮、雌二醇、罗格列酮）的蛋白水平以及卵泡抑素亚基（α、β A、β B）、卵泡抑素亚型（288和315）的mRNA水平。在正常的周期妇女雄烯二酮高，而雌二醇低FF的小卵泡。随着卵泡的生长，前者减少，后者增加。孕激素和白蛋白A均随时间延长而升高。卵泡生长，而卵泡抑素B、激活素A、游离卵泡抑素水平无显著变化。在mRNA水平上，卵泡抑素α和β A亚基均随卵泡生长而沿着增加，而卵泡抑素β B亚基和卵泡抑素288/315均无明显变化。在PCOS GCs中，与大小匹配的卵泡相比，两种β-氨基丁酸亚基-α和β-氨基丁酸亚基-α的NA水平均显著降低。其他mRNA表达差异不显著。这些结果提示，PCOS的病理生理过程中可能存在着BMPIN/activtn系统的参与。