The research of regeneration, differentiation and the role of fibroblast growth factor in the olfactory system
嗅觉系统再生、分化及成纤维细胞生长因子作用的研究
基本信息
- 批准号:12671653
- 负责人:
- 金额:$ 1.92万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Olfactory receptor cells are widely thought to regenerate after degeneration and also thought to show turnover under normal circumstances in the animal olfactory epithelium. The regeneration is carried out about 1 month after axotomy. Recently it was reported that stem cell migrated from the subventricular zone to the subependymal zone of the olfactory bulb through rostal migratory pathway. We studied cell division dynamics the guinea pig olfactory bulb using immunohistochemical double staining. Anti-bromodeoxyuridine (BrdU) antibody for detecting dividing cells, anti-neural cell adhesion molecule, anti-protein gene product antibody and anti-calbindin antibody, for neural cells, and anti S-100 antibody for glial cells were used immunohistochemical staining.At 1 hour after BrdU injection, many BrdU-labeled cells were present in the subventricular zone. BrdU-labeled cells migrated from the subventricular zone to the subependymal zone of the olfactory bulb through rostal migratory pathway within about 1 week. Labeled cells then moved peripherally from the bulb zone differentiated neural cells and glial cells in some layers.Our results support the idea that an adequate dose of bFGF plays an important role in the neurogenesis in the olfactory epithelium. Further study is needed to clarify the efficacy of bFGF in the applied olfactory cells, but at least bFGF was found to influence to the dividing activity in olfactory cells.
嗅觉受体细胞被广泛认为在变性后再生,并且也被认为在动物嗅觉上皮中在正常情况下显示更新。再生在轴突切断后约1个月进行。近年来研究发现,嗅球干细胞可通过吻侧迁移途径从室管膜下区迁移至嗅球室管膜下区。应用免疫组织化学双染法研究了豚鼠嗅球细胞分裂动力学。免疫组化染色:用抗溴脱氧尿苷(BrdU)抗体检测分裂细胞,用抗神经细胞粘附分子、抗蛋白基因产物抗体和抗钙结合蛋白抗体检测神经细胞,用抗S-100抗体检测神经胶质细胞。BrdU标记的细胞在1周内从嗅球室管膜下区通过喙部迁移途径迁移至嗅球室管膜下区。标记细胞从嗅球区向周围迁移,分化为神经细胞和神经胶质细胞。我们的结果支持了足够剂量的bFGF在嗅上皮神经发生中起重要作用的观点。bFGF对嗅细胞的作用还需进一步研究,但至少bFGF对嗅细胞的分裂活性有影响。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hideo Nakamura: "The effect of basic fibroblast growth factor on the regeneration of guinea pig olfactory epithelium"European Archieve of Otorhinolaryngology. 259. 166-169 (2002)
Hideo Nakamura:“碱性成纤维细胞生长因子对豚鼠嗅上皮再生的影响”欧洲耳鼻喉科档案馆。
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中村 英生: "嗅球内分裂細胞の動態"日本鼻科学会誌. (発表予定). (2002)
Hideo Nakamura:“嗅球分裂细胞的动力学”,日本鼻学会杂志(待出版)。
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- 影响因子:0
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Hideo Nakamura: "The effect of basic fibroblast growth factor on the regeneration of guinea pig's olfactory epithelium"European Archives of Oto-Rhino-Laryngology. (発表予定). (2002)
Hideo Nakamura:“碱性成纤维细胞生长因子对豚鼠嗅觉上皮再生的影响”欧洲耳鼻喉学档案(即将出版)。
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- 影响因子:0
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中村 英生: "モルモット嗅球内における分裂細胞の動態"日本鼻科学会誌. 41. 1-4 (2002)
Hideo Nakamura:“豚鼠嗅球分裂细胞的动力学”日本鼻学会杂志 41. 1-4 (2002)。
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- 影响因子:0
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Hideo Nakamura: "Cell dynamics of dividing cells in the olfactory bulb of guinea pigs"The Journal of Japan rhinologic society. Vol.41. 1-4 (2002)
Hideo Nakamura:“豚鼠嗅球分裂细胞的细胞动力学”日本鼻学会杂志。
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NAKAMURA Hideo其他文献
NAKAMURA Hideo的其他文献
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{{ truncateString('NAKAMURA Hideo', 18)}}的其他基金
Development of a novel therapy for malignant glioma based on theSNIP-microarray analysis and Glioma Cancer Stem cell theory
基于 SNIP 微阵列分析和神经胶质瘤干细胞理论开发恶性神经胶质瘤新疗法
- 批准号:
22591616 - 财政年份:2010
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of capacitively-coupled multi-channel surface EMG interface
电容耦合多通道表面肌电接口的开发
- 批准号:
21700587 - 财政年份:2009
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of computer interface based on neuromuscular system activity signal on multi-channel surface electromyograms
基于多通道表面肌电图神经肌肉系统活动信号的计算机接口开发
- 批准号:
19700453 - 财政年份:2007
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Investigation for the mechanism of the therapeutic resistance in central nervous system germ cell tumors based on the genetic & biological analysis
基于遗传学的中枢神经系统生殖细胞肿瘤耐药机制研究
- 批准号:
19591689 - 财政年份:2007
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analysis of malignant brain tumor related gene <h-neu> and development of a novel molecular tageting therapy based on the h-neu gene function
恶性脑肿瘤相关基因<h-neu>的功能分析及基于h-neu基因功能的新型分子标记疗法的开发
- 批准号:
17591520 - 财政年份:2005
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of the gene therapy for the malignant brain tumor using the oncolytic herpes virus vector
利用溶瘤疱疹病毒载体开发恶性脑肿瘤基因治疗
- 批准号:
15591535 - 财政年份:2003
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Proposal and Application of Evaluation System of "Level of City Life"
“城市生活水平”评价体系的提出及应用
- 批准号:
14550533 - 财政年份:2002
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Development of an Expert System for Diagnosis and Prescription of Traffic-Related Environment in Metropolises
大都市交通相关环境诊断与处方专家系统的开发
- 批准号:
10555185 - 财政年份:1998
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Relationship between heat treatment and fatigue crack extension properties demonstrated on the dental alloy casting used as the compact-type specimen
在用作致密型样品的牙科合金铸件上展示了热处理与疲劳裂纹扩展性能之间的关系
- 批准号:
10671820 - 财政年份:1998
- 资助金额:
$ 1.92万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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