Development of the gene therapy for the malignant brain tumor using the oncolytic herpes virus vector
利用溶瘤疱疹病毒载体开发恶性脑肿瘤基因治疗
基本信息
- 批准号:15591535
- 负责人:
- 金额:$ 1.15万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The vector which we have developed was termed HSV1yCD and an oncolytic vector. The most unique character of this virus vector was that it can selectively infect and lyse the tumor cell but not lyse the normal cell. In other words, it can selectively destroy the tumor cells infiltrating into the normal brain. The purpose of this project was to collect the experimental data about the oncolytic virus gene therapy against the brain tumor and to utilize the data for the future clinical gene therapy. Until the year 2003 we have confirmed that HSV1yCD vector can infect the tumor cells and efficiently produce cytosine deaminase which can convert 5-FC to 5-FU. We also have confirmed that 5-FU was effective drug against glioma cells in vitro. In the year 2004 we have challenged the in vivo study using the HSV1yCD. 9L and C6 glioma cells were injected into the rat brains and subsequently the HSV1yCD was injected into the brain. Several experimentally condition has been examined and the optimal experimental methods were established. We have examined the infectious efficacy of the HSV1yCD and the oncolytic activity. Compared the contol vector, HSV1yCD could efficiently destroy the tumor cells. We also have examined the survival rate of the rats injected the tumor cells and subsequently treated with the HSV1yCD and 5-FC. We concluded the HSV1yCD may be a effective vector for the treatment of the brain tumor. Although a further investigation should be required, the HSV1yCD could be a promising tool for the future clinical tool against the brain tumor.
我们开发的载体被命名为HSV1yCD和溶瘤载体。该病毒载体最独特的特点是可以选择性地感染和溶解肿瘤细胞,而不能溶解正常细胞。换句话说,它可以选择性地摧毁渗透到正常大脑中的肿瘤细胞。本课题的目的是收集溶瘤病毒基因治疗脑肿瘤的实验数据,为今后的临床基因治疗提供依据。直到2003年,我们已经证实HSV1yCD载体可以感染肿瘤细胞,并高效地产生胞嘧啶脱氨酶,将5-FC转化为5-FU。我们也证实了5-FU是体外抗胶质瘤细胞的有效药物。2004年,我们对使用HSV1yCD的体内研究提出了挑战。将9L和C6胶质瘤细胞注射到大鼠脑内,然后将HSV1yCD注射到脑内。考察了几种实验条件,确定了最佳实验方法。我们检测了HSV1yCD的感染效果和溶瘤活性。与对照载体相比,HSV1yCD能有效地杀伤肿瘤细胞。我们还检测了注射肿瘤细胞并随后用HSV1yCD和5-FC治疗的大鼠的存活率。结论HSV1yCD可能是治疗脑肿瘤的有效载体。虽然还需要进一步的研究,但HSV1yCD有望成为未来治疗脑肿瘤的临床工具。
项目成果
期刊论文数量(40)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Ushio Y.et al.: "Correlation of molecular genetic analysis of p53, MDM2, p16, PTEN, and EGFR and survival of patients with anaplastic astrocytoma and glioblastoma"Front Biosci.. 1(8). 281-288 (2003)
Ushio Y.等人:“p53、MDM2、p16、PTEN 和 EGFR 的分子遗传学分析与间变性星形细胞瘤和胶质母细胞瘤患者生存的相关性”Front Biosci.. 1(8)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Selectivity of an oncolytic herpes simplex virus for cells expressing the DF3/MUC1 antigen
- DOI:10.1158/0008-5472.can-03-3431
- 发表时间:2004-04-01
- 期刊:
- 影响因子:11.2
- 作者:Kasuya, H;Pawlik, TM;Tanabe, KK
- 通讯作者:Tanabe, KK
Contribution of angiotensin-converting enzyme and angiotensin II to ischemic stroke: their role in the formation of stable and unstable carotid atherosclerotic plaques.
- DOI:10.1016/j.surneu.2004.02.027
- 发表时间:2004-10
- 期刊:
- 影响因子:0
- 作者:M. Morioka;J. Hamada;A. Hashiguchi;Yu Hasegawa;T. Todaka;S. Yano;Y. Kai;M. Miura;S. Fujioka;Y. Ushio
- 通讯作者:M. Morioka;J. Hamada;A. Hashiguchi;Yu Hasegawa;T. Todaka;S. Yano;Y. Kai;M. Miura;S. Fujioka;Y. Ushio
Dephosphorylation of eNOS on Thr 495 after transient forebrain ischemia in gerbil hippocampus.
沙鼠海马短暂前脑缺血后,eNOS 在 Thr 495 上的去磷酸化。
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Hatakeyama S.;et al.;Hara T.et al.;Kaneko-Oshikawa C. et al.;A.Kamata;S.Moriguchi;F.Han;N.Shioda;H.Sakagami;Y.Hasegawa;S.Yano;A.Hashiguchi
- 通讯作者:A.Hashiguchi
Ischemia-induced neuronal cell death is mediated by the endoplasmic reticulum stress pathway involving CHOP
- DOI:10.1038/sj.cdd.4401365
- 发表时间:2004-04-01
- 期刊:
- 影响因子:12.4
- 作者:Tajiri, S;Oyadomari, S;Mori, M
- 通讯作者:Mori, M
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NAKAMURA Hideo其他文献
NAKAMURA Hideo的其他文献
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{{ truncateString('NAKAMURA Hideo', 18)}}的其他基金
Development of a novel therapy for malignant glioma based on theSNIP-microarray analysis and Glioma Cancer Stem cell theory
基于 SNIP 微阵列分析和神经胶质瘤干细胞理论开发恶性神经胶质瘤新疗法
- 批准号:
22591616 - 财政年份:2010
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of capacitively-coupled multi-channel surface EMG interface
电容耦合多通道表面肌电接口的开发
- 批准号:
21700587 - 财政年份:2009
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Development of computer interface based on neuromuscular system activity signal on multi-channel surface electromyograms
基于多通道表面肌电图神经肌肉系统活动信号的计算机接口开发
- 批准号:
19700453 - 财政年份:2007
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Investigation for the mechanism of the therapeutic resistance in central nervous system germ cell tumors based on the genetic & biological analysis
基于遗传学的中枢神经系统生殖细胞肿瘤耐药机制研究
- 批准号:
19591689 - 财政年份:2007
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Functional analysis of malignant brain tumor related gene <h-neu> and development of a novel molecular tageting therapy based on the h-neu gene function
恶性脑肿瘤相关基因<h-neu>的功能分析及基于h-neu基因功能的新型分子标记疗法的开发
- 批准号:
17591520 - 财政年份:2005
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Proposal and Application of Evaluation System of "Level of City Life"
“城市生活水平”评价体系的提出及应用
- 批准号:
14550533 - 财政年份:2002
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The research of regeneration, differentiation and the role of fibroblast growth factor in the olfactory system
嗅觉系统再生、分化及成纤维细胞生长因子作用的研究
- 批准号:
12671653 - 财政年份:2000
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Development of an Expert System for Diagnosis and Prescription of Traffic-Related Environment in Metropolises
大都市交通相关环境诊断与处方专家系统的开发
- 批准号:
10555185 - 财政年份:1998
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Relationship between heat treatment and fatigue crack extension properties demonstrated on the dental alloy casting used as the compact-type specimen
在用作致密型样品的牙科合金铸件上展示了热处理与疲劳裂纹扩展性能之间的关系
- 批准号:
10671820 - 财政年份:1998
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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Development of recombinant vaccines against canine protozoan diseases by using canine herpes virus vector
利用犬疱疹病毒载体研制犬原虫疾病重组疫苗
- 批准号:
13556049 - 财政年份:2001
- 资助金额:
$ 1.15万 - 项目类别:
Grant-in-Aid for Scientific Research (B)