Application of gene therapy on squamous cell carcinoma of the head and neck (SCCHN)

基因治疗在头颈鳞状细胞癌（SCCHN）中的应用

• 批准号: 12671685
• 负责人: ENDO Sohei
• 金额: $ 1.79万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671685/
• 关键词: squamous cell carcinoma; head and neck; gene therapy; antisense plasmid vector; transforming growth factor (TGF)-alpha; liposome; cisplatin

According to recent meta-analyses, chemotherapy has been proved to be beneficial in improving survival for the patients with squamous cell carcinoma of the head and neck (SCCHN). Cisplatin is the standard chemotherapeutic agent in treating SCCHN patients. Therefore cisplatin resistant is one of the major obstacles in improving survival. At first we established cisplatin-resistant SCCHN cell lines. Then we developed animal model where subcutaneously inoculated SCCHN tumor progresses even with cisplatin administration which were weekly injected intra-peritoneally (IP).On the other hand, we have also shown that antisense (AS) gene delivery targeting transforming growth factor (TGF)-α was effective in suppressing SCCHN tumor growth in vivo.This time we succeeded to show the results where simultaneous administration of TGF-α AS plasmid vector injected topically enhanced anti-tumor effects of cisplatin on SCCHN in vivo. This animal design provides a good preclinical model for the treatment of cisplatin-resistant SCCHN.

根据最近的荟萃分析，化疗已被证明对提高头颈部鳞状细胞癌(SCCHN)患者的生存率是有益的。顺铂是治疗SCCHN的标准化疗药物。因此，顺铂耐药是提高生存率的主要障碍之一。首先建立了顺铂耐药的SCCHN细胞系。另一方面，我们还证明了以转化生长因子-α为靶点的反义基因传递能有效地抑制小细胞肺癌的体内生长。这一次，我们成功地证明了在局部注射转化生长因子-α作为载体的同时，局部注射顺铂增强了顺铂对小细胞肺癌的抗肿瘤作用。该动物设计为顺铂耐药的SCCHN的治疗提供了良好的临床前模型。

项目成果

S Endo, et al.: "TGF-α antisense gene therapy inhibits head and neck aquamous cell carcinoma growth in vivo"Gene Therapy. 7. 1906-1914 (2000)

S Endo 等人：“TGF-α 反义基因治疗抑制体内头颈水状细胞癌生长”基因治疗。 7. 1906-1914 (2000)

S Endo, et al.: "TGF-α antisense gene therapy inhibits head and neck squamous cell carcinoma growth in vivo"Gene Therapy. 7. 1906-1914 (2000)

S Endo 等人：“TGF-α 反义基因疗法抑制体内头颈鳞状细胞癌生长”Gene Therapy 7. 1906-1914 (2000)。

S Endo, et al.: "TGF-α antisense gene therapy inhibits head and neck squamous cell carcinoma growth in vivo"Gene Ther.. 1906-1914 (2000)

S Endo 等人：“TGF-α 反义基因疗法抑制体内头颈鳞状细胞癌生长”Gene Ther. 1906-1914 (2000)

Endo S, et al.: "TGF-α antisense gene therapy inhibits head and neck squamous cell carcinoma growth in vivo"Gene Therapy. 7. 1906-1914 (2000)

Endo S 等人：“TGF-α 反义基因疗法抑制体内头颈鳞状细胞癌生长”基因疗法 7. 1906-1914 (2000)。