Research for clinical application of gene therapy for head and neck cancer

头颈癌基因治疗的临床应用研究

• 批准号: 14370543
• 负责人: NIBU Ken-ichi
• 金额: $ 8.77万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14370543/
• 关键词: head and neck cancer; gene therapy; adenoviral vector; COX-2; adenoviral vector; head and neck cancer; repication-selective; gene therpay; oncolysis; 頭頚部癌; 増殖型アデノウイルスベクター; CAR

Objective : To teat the oncolytic activity of COX2-promoter based conditional replication-selective adenovirus vector for squamous cell carcinoma cells of head and neck.Subjects : A conditional replication-selective adenovirus vector in which the expression of Ela, required for viral replication, is controlled by the COX-2 promoter, Ad-COX2-Ela, was generated. Its oncolytic activity according to the levels of COX-2 and CAR (Coxsackie and adenovirus receptor) expression was tested in a series of human head and neck squamous cell carcinoma cell lines.ResultsThe respective COX-2 mRNA expression ratios of KB, H891, T891, T892, and L871 were 1.5, 60, 1, 14.6, and 1.3. The corresponding CAR mRNA expression ratios were 1, 1, 5, 4, and 3. In vitro assays showed significant growth suppression of cancer cell lines with strong expressions of COX-2. In addition, Ad-COX2-Ela showed significant growth suppression of COX-2 expressing tumors in vivo model.CenclusionsIn this study, we demonstrated the possibility of oncolytic therapy using the COX-2 promoter based conditional replication-selective adenovirus for head dnd neck squamous cell carcinoma expressing COX-2.

目的：目的：研究考克斯-2启动子调控的条件复制选择性腺病毒载体（Ad-COX-2-Ela）对头颈部鳞癌细胞的杀伤活性。其溶瘤活性根据考克斯-2和CAR（科萨基和腺病毒受体）的表达水平进行了测试，在一系列的人头颈部鳞状细胞癌细胞line.ResultsThe相应的考克斯-2 mRNA表达率KB，H891，T891，T892，和L 871分别为1.5，60，1，14.6，和1.3。相应的CAR mRNA表达比率为1、1、5、4和3。体外试验显示，考克斯-2强表达的癌细胞系的生长受到显著抑制。此外，Ad-COX 2-Ela对表达考克斯-2的肿瘤有明显的生长抑制作用，提示利用基于考克斯-2启动子的条件复制选择性腺病毒治疗表达考克斯-2的头颈部鳞状细胞癌是可行的。

项目成果

RET/PTC fusion gene rearrangements in Japanese thyroid carcinomas

• DOI: 10.1007/s00405-004-0835-8
• 发表时间: 2005-05-01
• 期刊: EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
• 影响因子: 2.6
• 作者: Nibu, K;Otsuki, N;Rothstein, JL
• 通讯作者: Rothstein, JL

Partial laryngectomy to treat early glottic cancer after failure of radiation therapy

• DOI: 10.1001/archotol.128.8.909
• 发表时间: 2002-08-01
• 期刊: ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY
• 作者: Toma, M;Nibu, K;Sugasawa, M
• 通讯作者: Sugasawa, M

Intraductal papilloma arising from Stenson's duct

源自斯滕森导管的导管内乳头状瘤

• 期刊: J Otolaryngol (In press)(未定)
• 作者: Tanimoto H;et al.
• 通讯作者: et al.

A replication-selective adenoviral vector for head and neck cancers

• DOI: 10.1001/archotol.131.7.630
• 发表时间: 2005-07-01
• 期刊: ARCHIVES OF OTOLARYNGOLOGY-HEAD & NECK SURGERY
• 作者: Tanaka, H;Shirakawa, T;Nibu, K
• 通讯作者: Nibu, K

Adenovirsu-mediated gene transfer in olfactory epithelium and olfactgory bulb : a long-term study

腺病毒介导的嗅上皮和嗅球基因转移：一项长期研究

• 发表时间: 2005
• 期刊: Ann Otol Rhinol Laryngol 114
• 作者: Doi K;Nibu K;Ishida H;Okada H;Terashima T
• 通讯作者: Terashima T