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Nerve Fiber-circuits of Gustatory Sence

味觉神经纤维回路

基本信息

批准号：
12671776
负责人：
UEMURA Masanori
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671776/
关键词：
thalamus VPMpc insulacortex taste rat parabrachial nucleus insular cortex

项目摘要

Projections from the thalamic gustatory nucleus, i.e. the parvicellular part of the posteromedial ventral thalamic nucleus (VPMpc) to the forebrain regions were studied in the rat by the tract-tracing methods with anterograde tracer (biotinylated dextran amine: BDA) and anterogfade/retrograde tracer (wheat-germ aggluti ni n-horseradish peroxidase: WGA-HRP). After BDA injection into the VPMpc, terminal labeling was observed in the insular cortex, amygdaloid complex, and fundus striati. The terminal labeling in the amygdaloid complex was distributed in dorsolateral area of the rostral part of the lateral amygdaloid nucleus and the rostral part of the lateral subdivision of the central amygdaloid nucleus. The terminal labeling in the central amygdaloid nucleus extended to the fundus striati. The retrograde tracing study with WGA-HRP revealed that the projection fibers from the VPMpc to the amygdaloid complex originated from the medial part of the VPMpc and also from the thalamic area medial to the VPMpc. In the rats injected with Fluoro-Gold and WGA-HRP respectively into the insular cortex and amygdaloid complex, no double-labeled neuronal cell bodies were found in the VPMpc, although neurons labeled singly with Fluoro-Gold were intermingled with those singly labeled with WGA-HRP in the- medial part of the VPMpc. The results indicated that VPMpc neurons projecting to the amygdaloid complex constituted a population different from VPMpc neurons projecting to the insular cortex.

用顺行示踪剂(生物素化葡聚糖胺：BDA)和顺行/逆行示踪剂(小麦胚胶凝集蛋白-辣根过氧化物酶：WGA-HRP)，研究了大鼠丘脑味觉核，即丘脑腹侧后内侧核(VPMPC)的小细胞部向前脑区的投射。将BDA注入VPMPC后，在岛叶皮质、杏仁复合体和纹状眼底观察到终末标记。杏仁复合体内的终末标记分布在杏仁外侧核吻侧部的背外侧区和杏仁中央核外侧细分的吻侧部。杏仁中央核内的终末标记延伸至纹状体底。WGA-HRP逆行追踪研究表明，VPMPC向杏仁复合体的投射纤维来自VPMPC内侧部和丘脑内侧区至VPMPC。在岛叶皮质和杏仁复合体分别注射荧光金和WGA-HRP的大鼠，虽然在VPMpc的内侧部分，荧光金标记的神经元与WGA-HRP标记的神经元混合在一起，但在VPMpc内没有发现双标记的神经元胞体。结果表明，投射到杏仁复合体的VPMPC神经元与投射到岛叶皮质的VPMPC神经元构成了不同的群体。