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The Research of Reciprocal Connections to the Pathway of Gustatory and Rhinencephalons

味觉和鼻脑通路相互联系的研究

基本信息

批准号：
17591918
负责人：
UEMURA Masanori
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17591918/
关键词：
gustatory sensation olfaction VPMpc Single cell tracing virus tracer insular cortex amygdala amygdalostriatal transition area 嗅覚回路味覚回路 Sindbis virus 神経回路

项目摘要

In the present study, we aimed to reveal the interactions between the neural pathway. of gustatory sensation and brain areas related to olfaction. In our previous study, projections from the thalamic gustatory nucleus, i.e. the parvicellular part of the posteromedial ventral thalamic nucleus (VPMpc) to the forebrain regions were observed in the insular cortex, amygdaloid complex, and amygdalostriatal transition area (ASt). Thus we attempted to show how each neuron in the VPMpc project to these areas. We established the single cell tracing method with Sindbis virus vectors the coded membrane-targeted GFP, some neurons were labeled in a Golgi-like manner by the immunoperoxidase method with an anti-GFP antibody. After the reconstruction of projection axons, some neurons in VPMpc were observed to project their axons to only one area respectively. This result suggested that each neuron in the VPMpc had different character.Then we the attended to the amygdalostriatal transition area (ASt) that the fibers from the VPMpc mainly projected. The neurons of the ASt are consisted of the medium-sized spiny projection neurons are the neostriatal principal neurons. Although the neostriatum is considered to be an important region involved in the control of the voluntary movements, the neurons in the ASt are like to striatal projection neurons. So we now started the analysis of the relation between the ASt and the neostriatum in the gustatory sensation.

在本研究中，我们旨在揭示神经通路之间的相互作用。味觉和与嗅觉相关的大脑区域。在我们以前的研究中，在岛叶皮质、杏仁复合体和杏仁纹状体过渡区，观察到了丘脑味觉核，即丘脑后内侧腹侧核(VPMPC)的小细胞部向前脑区的投射。因此，我们试图展示VPMPC中的每个神经元是如何投射到这些区域的。我们建立了Sindbis病毒载体编码的膜靶向GFP的单细胞示踪方法，用抗GFP抗体的免疫过氧化物酶方法对部分神经元进行高尔基样标记。在重建投射轴突后，观察到VPMPC内的一些神经元仅将轴突投射到一个区域。这一结果提示VPMPC内的每个神经元具有不同的特性。然后，我们关注了来自VPMPC的纤维主要投射的杏仁核-纹状体过渡区。AST的神经元由中等大小的棘突投射神经元组成，是新纹状体的主要神经元。尽管新纹状体被认为是控制自主运动的重要区域，但AST中的神经元类似于纹状体投射神经元。因此，我们现在开始分析AST和新纹状体在味觉感觉中的关系。