权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The Role of Peroxisomes in Bone Formation

过氧化物酶体在骨形成中的作用

基本信息

批准号：
12671812
负责人：
FUJIWARA Satoko
金额：
$ 0.77万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671812/
关键词：
Peroxisomes Catalase MC3T3-E1 cell Uricase Epiphyseal cartilage D-aspartate oxidase D-amino acid oxidase Sucrose density gradient centrifugation ペルソキシソーム軟骨細胞

项目摘要

1. The morphology, distribution and biological function of peroxisomes in the fetal bovine articular cartilage of 5-month-old.Sucrose density gradient centrifugation of the homogenate from chondrocytes of the fetal bovine cartilage was performed.(1) The fetal bovine cartilage contained normal size peroxizomes with density of about 1.25, although it had been reported that the tissues out of liver and kidney contained microperoxisomes with low density.(2) Alanine : glyoxylate aminotransferase(AGT) which is only animotransferase in animal peroxisomes and is wide distributed in mammalian liver. AGT activity was not detected in peroxisomes of the fetal bovine cartilage, suggested that the function of peroxisomes in cartilage was different from liver.(3) D-aspartate oxidase(D-Asp O) was localized in peroxisomes in the fetal bovine cartilage, suggested that D-saparate was present in this tissue and D-Asp O was related to the developmental processes.2. The morphology, distribution and biological function of peroxisomes in MC3T3-E1 cell (osteoblast- like cell derived from mouse).Catalase activity was detected in the homogenate of MC3T3-E1 cells, suggested that peroxisomes were present in MC3T3-E1 cell. Other peroxisomal marker enzyme activities were not detected in the homogenate. Therefore, the proliferation of peroxisomes by clofibrate (peroxisomal proliferator) treatment and some peroxisomal enzyme activities must be investigated further.

1.5月龄胎牛关节软骨组织中过氧化体的形态、分布及生物学功能：(1)胎牛软骨含有正常大小的过氧化体，密度约为1.25，而肝、肾组织中含有密度较低的微体。(2)丙氨酸：乙二酸氨基转移酶(AGT)是动物过氧化酶体中唯一的一种酶，在哺乳动物肝脏中广泛分布。结论：1.胎牛软骨组织中未检测到AGT活性，提示软骨中的过氧化物体与肝脏中的功能不同。(3)D-天冬氨酸氧化酶(D-Asp O)定位于胎牛软骨中的过氧化物体中，提示D-天冬氨酸在该组织中存在，D-Asp O与发育过程有关。MC3T3-E1细胞(小鼠成骨样细胞)中过氧化物体的形态、分布和生物学功能。在MC3T3-E1细胞匀浆中检测到过氧化氢酶活性，表明MC3T3-E1细胞中存在过氧化物酶体。在匀浆中未检测到其他过氧化物体标记酶的活性。因此，必须进一步研究氯贝特(过氧化体增殖剂)对过氧化物体的增殖和某些过氧化物体酶活性的影响。