Diagnosis of oral cancer by ^<11>C-Choline PET

使用氟-18氟脱氧葡萄糖（FDG PET）的正电子发射断层扫描可用于检测恶性肿瘤复发和评估治疗反应。近年来，^<11>C-胆碱作为另一种肿瘤示踪剂被引入，并成功地实现了恶性肿瘤的显像。在本研究中，我们评估了^< 11b> c -胆碱PET在临床应用的可能性。HeLa S3细胞中^<11> c -胆碱摄取与细胞周期期的关系，以及它们与传统示踪剂FDG和^<11> c -蛋氨酸（Met）的比较。首先，我们构建了一个计算机控制的^<11> c -胆碱合成装置，用于PET的常规临床应用。2-二甲氨基乙醇装载到Sep-Pak Accell CM墨盒上，随后捕获^< 11bb0 c -甲基碘化物。结果表明，合成结束时^<11>C胆碱的平均量为140 mCi，放射化学纯度大于99%。该方法简便，使用一次性材料进行净化，有利于临床常规使用。与G1期相比，HeLa S3细胞在S期早期和G2/M期的FDG摄取显著增加。此外，在G2/M期，Met和^<11> c -胆碱摄取较高。我们认为，细胞周期依赖性反映在肿瘤组织PET成像中FDG、Met和^<11> c -胆碱的摄取上。这些结果揭示了肿瘤的增殖活性，并有助于评估治疗反应。进一步将^<11>C-Choline给予兔肿瘤模型，在PET上观察，准确确定肿瘤位点，并在^<11>C-Choline-PET上显示肿瘤可适用的影像学。

Positron emission tomography using fluorine-18 fluoro-deoxyglucose (FDG PET) is useful of the detection of malignant tumor recurrences and for the evaluation of a therapeutic response. Recently, ^<11>C- Choline was introduced as another tumor tracer and successfully visualized malignant tumor. In this research, we evaluated possibility to clinical application of ^<11>C-Choline PET. The relationship between ^<11>C-choline uptake and the cell cycle phase in HeLa S3 cells, as well as how they compare to the conventional tracer FDG and ^<11>C-Methionine (Met)At first we constructed a computer -controlled apparatus of ^<11>C-Choline synthesis for routine clinical application of PET. 2-dimethylaminoethanol was loaded onto a Sep-Pak Accell CM cartridge, subsequent to the trapping of ^<11>C-methyl iodide. As a result, the average amount of ^<11>C Choline at end of synthesis was 140 mCi, the radiochemical purity was more than 99%. The simplicity of this method and the use of disposable materials for purification way be advantageous for routine clinical use. FDG uptake in HeLa S3 cells was significantly higher in the early S phase and G2/M phase compared to the G1 phase. In addition, Met and ^<11>C-Choline uptakes were higher in the G2/M phase. It has been concluded cell cycle dependency is reflected in the uptake of FDG, Met and ^<11>C-Choline seen during PET imaging of tumor tissue. These results reveal tumor proliferative activity, and can assist in evaluating a therapeutic response.Furthermore, ^<11>C-Choline was administered to a rabbit neoplasm model, and it was observed in PET, and neoplasm locus was identified precisely, and what imaging of neoplasm could apply was shown in ^<11>C-Choline-PET.