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IMPROVEMENT AND DEVELOPMENT FOR IMPLANT MATERIALS USING HUMAN GINGIVAL EPITHELIAL CELLS

利用人牙龈上皮细胞的种植材料的改进和开发

基本信息

批准号：
12671910
负责人：
OGUCHI Hitoshi
金额：
$ 1.47万
依托单位：
TSURUMI UNIVERSITY SCHOOL OF DENTAL MEDICINE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671910/
关键词：
In Vitro human epithelial gigiva cells hydroxyapatite TEM SEM ヒドロキアパタイトヒト歯肉細胞 IN VITRO

项目摘要

In 1991, Ishikawa established a human epithelial cell line from human gingiva and named it HGE-15. Since we have studied the biocompatibility for various biomaterials using HGE-15 cells. The results of this from 2000 to 2001 are as follows ;1. In 2000, we have investigated mainly the biocompatibility the coating hydroxyapatie (HA) indicated high crystallization offered from ADVANCE Co. ltd. As a result, we have given a new knowledge that its surface structure has advantage compared with other coating HA in early stage.2. In 2001, we have added some other biomaterials (commercial titanium, HA impacted titanium, porous HA, high-velocity frame HA, plasma HA, and Falcon) and studied ones using HGE-15 cells and MC3T3 cells and human bone cells as same experimental system. Though we have analyzed the results at present, we have understood that the biocompatibility for various biomaterials has differed by using cell type. Furthermore, we and the biocompatibility of HA materials are poor compa … More red with the titanium and Falcon, and start the cell adhesion to adsorb some protein in high-velocity frame HA in early stage etc by transmission electron microscopy (TEM) and scanning electron microscopy (SEM).I have partly reported these results in invited lecture at the 7th Biomimetic Engineering ; A new Role for Ceramics (June, 14 Faenza, Italy). Furthermore, At present, I have carried out the cooperation and development and investigated the biological studies of the new biomaterials that offered NIRIN and National Institute for Ceramic Technology Italian National Research Council.Five studies have examined human gingival cells in vitro as present. Most previous cell culture studies regarding the attachment of gingival epithelial cells and various biomaterials have used mainly primary cells ; however, adhesion between these cells and biomaterials are insufficient. In 1991, Ishikawa claimed that although SGL cells, it remains questionable whether they have the same properties as gingival epithelial cells and newly established a human gingival epithelial cell line.This system and results should provide a lot of suggestion with regard to the mechanism by which epithelial cells adhere to various biomaterials in vivo and clinic. Less

1991年，石川从人类牙龈中建立了人类上皮细胞系，并将其命名为HGE-15。由于我们利用HGE-15细胞研究了各种生物材料的生物相容性。2000 - 2001年的研究结果如下：2000年，我们主要研究了ADVANCE公司提供的具有高结晶性的羟基磷灰石涂层的生物相容性。因此，我们给出了一个新的认识，即它的表面结构在早期与其他涂层HA相比具有优势。2001年，我们加入了一些其他生物材料（商用钛、HA冲击钛、多孔HA、高速框架HA、等离子HA、Falcon），并将HGE-15细胞和MC3T3细胞与人骨细胞作为同一实验系统进行了研究。虽然我们目前对结果进行了分析，但我们了解到不同的生物材料的生物相容性因细胞类型的不同而不同。此外，通过透射电镜（TEM）和扫描电镜（SEM）观察，我们与羟基磷灰石材料的生物相容性较差，与钛和猎鹰相比更红，并在高速框架羟基磷灰石的早期阶段开始细胞粘附以吸附一些蛋白质等。我在第七届仿生工程大会的特邀演讲中部分报告了这些结果；陶瓷的新角色（6月14日，法恩扎，意大利）。此外，目前，我已经开展了合作和开发，并调查了新的生物材料，提供了NIRIN和国家陶瓷技术研究所意大利国家研究委员会的生物学研究。目前已有五项研究在体外检测了人类牙龈细胞。以往关于牙龈上皮细胞与各种生物材料附着的细胞培养研究大多采用原代细胞；然而，这些细胞与生物材料之间的粘附性是不够的。1991年，Ishikawa声称，虽然SGL细胞，但其是否具有与牙龈上皮细胞和新建立的人类牙龈上皮细胞系相同的特性仍然值得怀疑。该系统和结果将为研究上皮细胞在体内和临床中粘附各种生物材料的机制提供许多建议。少