Development if a new bone implantation method using recombinant BMP-2 (induced bone implantation)

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1. We implanted bone induced by recombinant BMP-2 (rhBMP-2) or freeze-dried allogenic bone to bone defect and compared the restoration state.2. Collagen was used as carrier for rhBMP-2 (50 ug), the specimens were freeze-dried, and pellets (4 mm in diameter) were prepared. These pellets were implanted into the rectus abdominis muscle or rats in the induced bone implantation group, and soft X-ray films were serially obtained. After 6 weeks, new bone formed in the rectus abdominis muscle was removed. A bone defect (4 mm in diameter) was prepared in the skull of the same rats, and the induced bone was implanted into the defect.3. Bone pieces (4 mm in diameter) collected from the skull of killed rats were freeze-dried, and bone grafts in the allogenic bone implantation group were prepared. The bone graft was implanted into a bone defect (4 mm in diameter) prepared in the skull of rats in the allogenic bone implantation group.4. Six weeks after grafting, the rats in both groups were killed, … More and the graft area was removed, stained with H-E, and observed by microscopy.5. In the induced bone implantation, fibrous tissue and connective tissue containing capillaries were present between the graft bone and the recipient bed bone, and new trabecular growth from the recipient bed bone was partially observed specificly in areas adjacent to the recipient and graft bones. Six weeks after implantation, capillaries in the fibrous tissue between the recipient bed bone and graft bone decreased, and new trabecular growth from the margin of the recipient bed bone became more marked, and new bone connecting the graft bone with recipient bed bone was observed in areas close to the two bone. Nine weeks after implantation, new trabecular growth was observed in the entire periphery on the recipient bed bone side, and solitary bone tissue was also present in areas where the graft bone is separated from the recipient bed bone. There were no findings suggesting resorption of the graft bone.In the allogenic bone implantation group, the area between the graft bone and recipient bed bone was filled only with fibrous connective tissue, and no bone growth from the recipient bed bone was observed 3, 6, or 9 weeks after implantation.6. These result suggest the usefulness of BMP-2 induced bone implantation compared to allogenic bone implantation. Less