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Investigation of Enviromental Factors for Oral Cancer Screening

口腔癌筛查的环境因素调查

基本信息

批准号：
12671964
负责人：
YAMANE Gen-yuki
金额：
$ 1.66万
依托单位：
TOKYO DENTAL COLLEGE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671964/
关键词：
Oral Cancer Epithelial Dysplasia p53 gene early diagnosis 口腔上皮異形成色素染色異型上皮

项目摘要

A study was conducted in rats with early tongue carcinoma induced by 4-nitroquinoline 1-oxide (4NQO), in order to investigate the early diagnosis of malignant potential of epithelial dysplasia.The rat tongue lesions were classified by their severity into three groups corresponding to early cancer, dysplasia and no change. The grade of epithelium was determined according to 13 items of WHO Epithelial Dysplasia Criteria.The expression levels of p53 and Bcl-2 proteins were detected immunohistochemically, and apoptotic cells were detected using the TUNEL method. In addition, a p53 mulation by lesions was detected. The expression ratio of p53 protein was high in dysplasia, and the ratio of Bcl-2 protein was high in early cancer and dysplasia. The TUNEL-positive cells were obscrved primarily in the granular layers of the no change cells, and their numbers decreased as the cells shifted to the early cancer stage. The p53 mutation was detected using a microdissection method in dysplasia, where it was found in three out of nine lesions. All the mutations in dysplasia detected were on the same codon that was found to be mutated in the early cancer.These results indicate that the association between the p53 mutation and histological changes in canceration of epithelial dysplasia is strong, and that both the identification of p53- and Bcl-2- positive epithelium, and decrease in the TUNEL positive ratio, were useful for the diagnosis of the malignant potential of precancerous lesions.

为了探讨上皮不典型增生恶性潜能的早期诊断，在4-硝基喹啉1-氧化物（4NQO）诱导的早期舌癌大鼠中进行了一项研究。将大鼠舌病变按严重程度分为三组，分别对应于早期癌、不典型增生和无变化。根据WHO上皮不典型增生标准13项进行上皮分级。免疫组化法检测p53、Bcl-2蛋白表达水平，TUNEL法检测凋亡细胞。此外，还检测到了病变的 p53 表达。 p53蛋白在不典型增生中表达比例较高，Bcl-2蛋白在早期癌和不典型增生中表达比例较高。 TUNEL阳性细胞主要在无变化细胞的颗粒层中观察到，并且随着细胞进入早期癌症阶段，其数量减少。 p53 突变是通过显微解剖方法在不典型增生中检测到的，在九个病变中发现了三个。所有检测到的不典型增生突变均与早期癌症中发现的突变密码子相同。这些结果表明，p53突变与上皮不典型增生癌变的组织学变化之间有很强的相关性，p53和Bcl-2阳性上皮的鉴定以及TUNEL阳性率的下降有助于诊断癌前病变的恶性潜力。