Study on the Influence of Environmental Hormone to Cleft Lip and/or Palate

目的：产妇环境因素对唇腭裂的发生有重要影响。在clp易发A/J品系小鼠中，雌二醇可降低唇裂和/或腭裂的发生率，提示多种激素影响面部生成。环境激素与女性激素类似，对人体的影响受到关注。因此，我们研究了环境激素对唇腭裂的影响。方法：采用A/J系小鼠（日本SLC）进行实验。由于在妊娠第9天给药时，唇裂和/或腭裂的发生率最低，因此在妊娠第9天给药BisPhenolA。BisPhenolA给药量分别为0、0.1、10mg/kg。在妊娠第18天调查唇腭裂的发生率。结果：对照组唇腭裂发生率为7.82%。1.01mg/kg组唇腭裂发生率分别为4.08%、1.96%和7.31%，0.1mg/kg组、1mg/kg组和10mg/kg组唇腭裂发生率分别为4.91%、4.91%和7.31%。0.01mg/kg双酚ola组和0.1mg/kg双酚ola组唇腭裂发生率降低，但随剂量增加唇腭裂发生率增加。讨论：双酚ola分子非常小，可以通过胎盘到达胎儿。它与雌激素受体结合，起到雌性激素的作用。由于颅神经嵴细胞在颌面发育中起着重要的作用，其运动或生长的中断会导致唇裂和/或腭裂等先天性异常。我们认为BisPhenolA给药后9天胎儿神经嵴细胞的移动基本完成，BisPhenolA作用于细胞，促进其增殖。此外，大量的环境激素会导致先天性畸形，如唇裂和/或腭裂。因此，0.1mg/kg给药组对唇腭裂发生率有抑制作用，但1mg/kg和10mg/kg给药组表现出致畸性，唇腭裂发生率增高。少

Purpose : Maternal environmental factors have much influence on the occurrence of cleft lip and/or palate. As incidence of cleft lip and/or palate in CLP-prone A/J strain mouse decreases by administrating estradiol, it is suggested that various hormones affect facial generation. As environmental hormone acts like female hormone, its influence to human body is concerned. Therefore, we studied about the influence of environmental hormone to cleft lip and/or palate.Method : We used A/J strain mouse (Japan SLC) for experiment. As incidence of cleft lip and/or palate decreases to a minimum when estrogen is administered on the 9^<th> gestation day, BisPhenolA was administered on the 9_<th> gestation day. Amount of BisPhenolA administered was 0, 0.1, 10mg/kg each. Incidence of cleft lip and/or palate was investigated on the 18^<th> gestation day.Result : Incidence of cleft lip and/or palate in the control group was 7.82%. Incidence of cleft lip and/or palate in 1.01mg/kg group was 4.08%, 1.96 … More % in 0.1mg/kg group, 4.91% in 1mg/kg group and 7.31% in 10mg/kg group. Though incidence of cleft lip and/or palate decreased in the group administered 0.01mg/kg BisPhenolA and 0.1mg/kg group, incidence increased as amount increased.Discussion : Molecular of BisPhenolA is so small that it reaches fetus through placenta. It combines with estrogen receptor and acts like female hormone. As cephalo neural crest cell has an important role in maxillofacial generation, it is well known that interruption of its move or growth causes congenital anomalies like cleft lip and/or palate. It was considered that move of neural crest cell is almost completed in the BisPhenolA administered fetus at 9^<th> day, BisPhenolA acted to the cell and promoted its proliferation. Also, environmental hormone in large amounts induces congenital anomalies like cleft lip and/or palate. Therefore, it suppressed incidence of cleft lip and/or palate for 0.1mg/kg administered group, but it showed teratogenicity for 1mg/kg and 10mg/kg groups and incidence of cleft lip and/or palate increased. Less