Study on the Influence of Environmental Hormone to Cleft Lip and/or Palate

目的：母体环境因素对唇腭裂的发生有很大影响。在易患CLP的A/J品系小鼠中，给予雌二醇可降低唇裂和/或腭裂的发生率，这表明不同的激素影响面部发育。由于环境激素的作用类似于女性激素，其对人体的影响备受关注。因此，我们研究了环境激素对唇腭裂的影响。方法：采用A/J系小鼠(日本SLC)进行实验。当在妊娠第9天使用雌激素时，唇裂和/或腭裂的发生率降至最低，因此在妊娠第9天使用双酚A。双酚A剂量分别为0、0.1、10 mg/kg。结果：对照组唇腭裂发生率为7.82%。1.01 mg/kg组唇腭裂发生率为4.08%，1.96…0.1 mg/kg组、1 mg/kg组、10 mg/kg组分别为4.91%、7.31%。双酚A 0.01 mg/kg组和0.1 mg/kg组唇腭裂的发生率虽有所下降，但随着剂量的增加，双酚A的发生率呈上升趋势。它与雌激素受体结合，起到女性荷尔蒙的作用。由于头神经嵴细胞在颌面部发育过程中起着重要的作用，其运动或生长受阻会导致唇腭裂等先天性畸形，这是众所周知的。认为双酚A作用于第9天的胎儿神经脊细胞运动已基本完成，双酚A作用于神经脊细胞并促进其增殖。此外，大量的环境荷尔蒙会导致先天性畸形，如唇裂和/或腭裂。因此，0.1 mg/kg剂量组可抑制唇腭裂的发生，而1 mg/kg和10 mg/kg剂量组则表现出致畸作用，唇腭裂发生率升高。较少

Purpose : Maternal environmental factors have much influence on the occurrence of cleft lip and/or palate. As incidence of cleft lip and/or palate in CLP-prone A/J strain mouse decreases by administrating estradiol, it is suggested that various hormones affect facial generation. As environmental hormone acts like female hormone, its influence to human body is concerned. Therefore, we studied about the influence of environmental hormone to cleft lip and/or palate.Method : We used A/J strain mouse (Japan SLC) for experiment. As incidence of cleft lip and/or palate decreases to a minimum when estrogen is administered on the 9^<th> gestation day, BisPhenolA was administered on the 9_<th> gestation day. Amount of BisPhenolA administered was 0, 0.1, 10mg/kg each. Incidence of cleft lip and/or palate was investigated on the 18^<th> gestation day.Result : Incidence of cleft lip and/or palate in the control group was 7.82%. Incidence of cleft lip and/or palate in 1.01mg/kg group was 4.08%, 1.96 … More % in 0.1mg/kg group, 4.91% in 1mg/kg group and 7.31% in 10mg/kg group. Though incidence of cleft lip and/or palate decreased in the group administered 0.01mg/kg BisPhenolA and 0.1mg/kg group, incidence increased as amount increased.Discussion : Molecular of BisPhenolA is so small that it reaches fetus through placenta. It combines with estrogen receptor and acts like female hormone. As cephalo neural crest cell has an important role in maxillofacial generation, it is well known that interruption of its move or growth causes congenital anomalies like cleft lip and/or palate. It was considered that move of neural crest cell is almost completed in the BisPhenolA administered fetus at 9^<th> day, BisPhenolA acted to the cell and promoted its proliferation. Also, environmental hormone in large amounts induces congenital anomalies like cleft lip and/or palate. Therefore, it suppressed incidence of cleft lip and/or palate for 0.1mg/kg administered group, but it showed teratogenicity for 1mg/kg and 10mg/kg groups and incidence of cleft lip and/or palate increased. Less