Study of Environmental Factor on Occurrence of Cleft Lip and/or Palate by Fertilized Ovum Transplantation

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I. We carried out transplantation and implantation of fertilized ovum between the same strain mice and between the different strain mice and studied about the prevalence of congenital anomalies such as cleft lip and/or palate, cardiovascular and thymic anomalies. We obtained the following results.1. Fertilized ova of pro-CLP strain transplanted to anti-CLP strain was suppressed the occurrence of CLP, cardiovascular and thymic anomalies.2. Fertilized ova of anti-CLP strain transplanted to pro-CLP strain did not cause congenital anomalies.3. It is suggested that about the multiple anomaly within high incidence family, multi-generic diseases can be suppressed by environmental changes.II. We administered folic acid to A/J strain mice with the amount equivalent to 0.8mg/day per a person between the period of 2 weeks before pregnancy and 13 gestation day, and 0.4mg/day per a person between the period of 14 gestation day and 18 gestation day. We studied about the influence of folk acid to the … More fetus body weight and incidence of CLP among fetuses and obtained the following results.1. Resorption rate was 12.3% in the control group and 10.2% in the experimental group (0.8mg administered) respectively, showing no significant difference between them.2. About the average weight of fetuses, while both experimental groups (10mg administered and 1mg administered) decreased significantly compared to the control group, experimental group (0.8mg administered) did not decrease, not showing significant difference compared to the control group.3. While the incidence of CP only of 10mg group and 1mg group decreased showing significant difference compared to the control group, 0.8mg group showed no suppression effect.4. Incidence of CL/P in all of 10mg, 1mg and 0.8mg experimental groups did not decrease showing no significant differences compared to the control group.These results suggest that 0.8mg administration of folic acid on the period from pre-pregnancy to organogenesis, 0.4mg administration of folic acid on fetus growing stage can prevent weight decrease. Further study is thought to make it possible to prevent CL/P. Less