Study of roles of EP receptors and their clinical application in periodontal disease
EP受体在牙周病中的作用及其临床应用研究
基本信息
- 批准号:12672029
- 负责人:
- 金额:$ 2.5万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2000
- 资助国家:日本
- 起止时间:2000 至 2001
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We investigated the roles of prostaglandin E_2 (PGE_2) receptors, which are divided into EP1, EP2, EP3, EP4, in human gingival fibroblasts (HGF) and periodontal ligament (PDL) cells. HGF and PDL cells were stimulated with IL-1β and IL-1α, respectively, and produced IL-6 and PGE_2. Inhibition of endogenous PGE_2 by indomthacin, a cyclooxygenase inhibitor, enhanced IL-1-induced IL-6 production, although it completely inhibited IL-1-induced PGE_2 production. Exogenous PGE_2 suppressed IL-1-induced IL-6 production in both types of cells. 11-deoxy-PGE_1, a selective EP2/EP3/EP4 agonist, and butaprost, a selective EP2 agonist, inhibited IL-1-induced IL-6 production, although butaprost was less potent than 11-deoxy-PGE_1. ONO-AP-324, an EP3 agonist, could not affect IL-1-induced IL-6 production. 17-phenyl-ω-trinor PGE_2 enhanced IL-1-induced IL-6 production in HGF. Reverse transcription-polymerase chain reaction analysis demonstrated mRNA expression of EP2 and EP4 in PDL cells. Dibutyryl cAMP, a cAMP analog, and forskolin, an activator of adenylate cyclases, reduced IL-6 production by IL-1-stimulated cellsIn conclusion, we suggest that PGE_2 downregulates IL-1-induced IL-6production via EP2/EP4 receptors by cAMP-dependent pathways in both HGF and PDL cells. EP2 and/or EP4 receptors may play critical roles in controlling inflammatory periodontal conditions.
本实验研究了前列腺素E_2(PGE_2)受体(EP 1、EP 2、EP 3、EP 4)在人牙龈成纤维细胞(HGF)和牙周膜细胞(PDL)中的作用。用IL-1β和IL-1α分别刺激HGF和PDL细胞,产生IL-6和PGE_2。环氧化酶抑制剂indomthacin抑制内源性PGE_2的产生,可增强IL-1诱导的IL-6的产生,但完全抑制IL-1诱导的PGE_2的产生。外源性PGE_2抑制IL-1诱导的两种细胞IL-6的产生。选择性EP 2/EP 3/EP 4激动剂11-deoxy-PGE_1和选择性EP 2激动剂butaprost抑制IL-1诱导的IL-6产生,但butaprost的作用不如11-deoxy-PGE_1。EP 3激动剂ONO-AP-324不能影响IL-1诱导的IL-6产生。17-phenyl-ω-trinor PGE_2增强IL-1诱导的HGF产生IL-6。逆转录-聚合酶链反应(RT-PCR)分析显示,PDL细胞中EP 2和EP 4的mRNA表达。cAMP类似物Dibutyryl cAMP和腺苷酸环化酶激活剂forskolin均能抑制IL-1刺激的细胞产生IL-6。EP 2和/或EP 4受体可能在控制炎症性牙周疾病中起关键作用。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Noguchi K.: "Prostaglandin F_<2α> upregulates interleukin-6 production in human gingival fibroblasts"J Periodont Res. 36. 80-87 (2001)
Noguchi K.:“前列腺素 F_<2α> 上调人牙龈成纤维细胞中白细胞介素 6 的产生”J periodont Res. 36. 80-87 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Noguchi, K: "Involvement of cyclooxygenase-2 in serum-induced prostaglandin production by human oral gingival epithelial cells"J Periodont Res. 36. 124-130 (2001)
Noguchi, K:“环氧合酶-2 参与血清诱导的人口腔牙龈上皮细胞产生前列腺素”J 牙周研究。
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- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
Noguchi K.: "Prostaglandin F_<2α> to upregulaters matrix metalloproteinase-1 production in human gingival fibroblasts"J Periodont Res. 36. 334-339 (2001)
Noguchi K.:“前列腺素F_<2α>对人牙龈成纤维细胞中基质金属蛋白酶-1产生的上调”J periodont Res. 36. 334-339 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Noguchi, K: "Prostaglandin F2α upregulates interleukin-6 production in human gingival fibroblasts"J Periodont Res. 36. 80-87 (2001)
Noguchi, K:“前列腺素 F2α 上调人牙龈成纤维细胞中白细胞介素 6 的产生”J periodont Res. 36. 80-87 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kazuyuki Noguchi et al.: "Upregulation of matrix metalloproteinase-1 production by prostaglandin F_<2α> in human gingival fibroblasts"J Periodont Res. (印刷中).
Kazuyuki Noguchi 等人:“人牙龈成纤维细胞中前列腺素 F_<2α> 对基质金属蛋白酶-1 产生的上调”J periodont Res。
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- 影响因子:0
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NOGUCHI Kazuyuki其他文献
Analysis of short-term cardiac and renal disorders in mice with cardio-renal damages
心肾损伤小鼠短期心肾功能紊乱分析
- DOI:
- 发表时间:
2022 - 期刊:
- 影响因子:0
- 作者:
NOGUCHI Kazuyuki;ISHIDA Junji;MUROMACHI Naoto;AKIYAMA Tomoki;Kim Jun-Dal;USUI Joichi;YAMAGATA Kunihiro;and FUKAMIZU Akiyoshi - 通讯作者:
and FUKAMIZU Akiyoshi
NOGUCHI Kazuyuki的其他文献
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{{ truncateString('NOGUCHI Kazuyuki', 18)}}的其他基金
Development of novel periodontal/bone regenerative therapy utilizing LIPUS, BMP9, and dedifferentiated fat cells
利用 LIPUS、BMP9 和去分化脂肪细胞开发新型牙周/骨再生疗法
- 批准号:
19K10169 - 财政年份:2019
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of new regeneration therapy using strong osteoplasty protein BMP-9 and cells derived from adipose tissue
使用强骨形成蛋白 BMP-9 和脂肪组织来源的细胞开发新的再生疗法
- 批准号:
15H05036 - 财政年份:2015
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of the dental implant with a periodontal tissue using stem cells
利用干细胞开发带有牙周组织的牙种植体
- 批准号:
23659922 - 财政年份:2011
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
A comprehensive study on periodontal regeneration using induced pluripotent stem(iPS) cells
使用诱导多能干(iPS)细胞进行牙周再生的综合研究
- 批准号:
21390525 - 财政年份:2009
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation of cementoblast differenciation by transcriptional factors and periodontal regeneration
转录因子对成牙骨质细胞分化和牙周再生的调节
- 批准号:
19592381 - 财政年份:2007
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Elucidation of roles and functions of cementoblasts in the destruction and regeneration of periodontal tissue
阐明成牙骨质细胞在牙周组织破坏和再生中的作用和功能
- 批准号:
15592185 - 财政年份:2003
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Cytological and pharmacological studies on the pathogenesis of nifedipine-associated gingival hyperplasia
硝苯地平相关牙龈增生发病机制的细胞学和药理学研究
- 批准号:
09671945 - 财政年份:1997
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The roles of cyclooxygenase-2 in the pathogenesis of periodontal disease
环氧合酶2在牙周病发病机制中的作用
- 批准号:
07672073 - 财政年份:1995
- 资助金额:
$ 2.5万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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