Study of roles of EP receptors and their clinical application in periodontal disease

EP受体在牙周病中的作用及其临床应用研究

基本信息

  • 批准号:
    12672029
  • 负责人:
  • 金额:
    $ 2.5万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

We investigated the roles of prostaglandin E_2 (PGE_2) receptors, which are divided into EP1, EP2, EP3, EP4, in human gingival fibroblasts (HGF) and periodontal ligament (PDL) cells. HGF and PDL cells were stimulated with IL-1β and IL-1α, respectively, and produced IL-6 and PGE_2. Inhibition of endogenous PGE_2 by indomthacin, a cyclooxygenase inhibitor, enhanced IL-1-induced IL-6 production, although it completely inhibited IL-1-induced PGE_2 production. Exogenous PGE_2 suppressed IL-1-induced IL-6 production in both types of cells. 11-deoxy-PGE_1, a selective EP2/EP3/EP4 agonist, and butaprost, a selective EP2 agonist, inhibited IL-1-induced IL-6 production, although butaprost was less potent than 11-deoxy-PGE_1. ONO-AP-324, an EP3 agonist, could not affect IL-1-induced IL-6 production. 17-phenyl-ω-trinor PGE_2 enhanced IL-1-induced IL-6 production in HGF. Reverse transcription-polymerase chain reaction analysis demonstrated mRNA expression of EP2 and EP4 in PDL cells. Dibutyryl cAMP, a cAMP analog, and forskolin, an activator of adenylate cyclases, reduced IL-6 production by IL-1-stimulated cellsIn conclusion, we suggest that PGE_2 downregulates IL-1-induced IL-6production via EP2/EP4 receptors by cAMP-dependent pathways in both HGF and PDL cells. EP2 and/or EP4 receptors may play critical roles in controlling inflammatory periodontal conditions.
本实验研究了前列腺素E_2(PGE_2)受体(EP 1、EP 2、EP 3、EP 4)在人牙龈成纤维细胞(HGF)和牙周膜细胞(PDL)中的作用。用IL-1β和IL-1α分别刺激HGF和PDL细胞,产生IL-6和PGE_2。环氧化酶抑制剂indomthacin抑制内源性PGE_2的产生,可增强IL-1诱导的IL-6的产生,但完全抑制IL-1诱导的PGE_2的产生。外源性PGE_2抑制IL-1诱导的两种细胞IL-6的产生。选择性EP 2/EP 3/EP 4激动剂11-deoxy-PGE_1和选择性EP 2激动剂butaprost抑制IL-1诱导的IL-6产生,但butaprost的作用不如11-deoxy-PGE_1。EP 3激动剂ONO-AP-324不能影响IL-1诱导的IL-6产生。17-phenyl-ω-trinor PGE_2增强IL-1诱导的HGF产生IL-6。逆转录-聚合酶链反应(RT-PCR)分析显示,PDL细胞中EP 2和EP 4的mRNA表达。cAMP类似物Dibutyryl cAMP和腺苷酸环化酶激活剂forskolin均能抑制IL-1刺激的细胞产生IL-6。EP 2和/或EP 4受体可能在控制炎症性牙周疾病中起关键作用。

项目成果

期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Noguchi K.: "Prostaglandin F_<2α> upregulates interleukin-6 production in human gingival fibroblasts"J Periodont Res. 36. 80-87 (2001)
Noguchi K.:“前列腺素 F_<2α> 上调人牙龈成纤维细胞中白细胞介素 6 的产生”J periodont Res. 36. 80-87 (2001)
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    0
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Noguchi, K: "Involvement of cyclooxygenase-2 in serum-induced prostaglandin production by human oral gingival epithelial cells"J Periodont Res. 36. 124-130 (2001)
Noguchi, K:“环氧合酶-2 参与血清诱导的人口腔牙龈上皮细胞产生前列腺素”J 牙周研究。
  • DOI:
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    0
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  • 通讯作者:
Noguchi K.: "Prostaglandin F_<2α> to upregulaters matrix metalloproteinase-1 production in human gingival fibroblasts"J Periodont Res. 36. 334-339 (2001)
Noguchi K.:“前列腺素F_<2α>对人牙龈成纤维细胞中基质金属蛋白酶-1产生的上调”J periodont Res. 36. 334-339 (2001)
  • DOI:
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  • 影响因子:
    0
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Noguchi, K: "Prostaglandin F2α upregulates interleukin-6 production in human gingival fibroblasts"J Periodont Res. 36. 80-87 (2001)
Noguchi, K:“前列腺素 F2α 上调人牙龈成纤维细胞中白细胞介素 6 的产生”J periodont Res. 36. 80-87 (2001)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kazuyuki Noguchi et al.: "Upregulation of matrix metalloproteinase-1 production by prostaglandin F_<2α> in human gingival fibroblasts"J Periodont Res. (印刷中).
Kazuyuki Noguchi 等人:“人牙龈成纤维细胞中前列腺素 F_<2α> 对基质金属蛋白酶-1 产生的上调”J periodont Res。
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NOGUCHI Kazuyuki其他文献

Analysis of short-term cardiac and renal disorders in mice with cardio-renal damages
心肾损伤小鼠短期心肾功能紊乱分析
  • DOI:
  • 发表时间:
    2022
  • 期刊:
  • 影响因子:
    0
  • 作者:
    NOGUCHI Kazuyuki;ISHIDA Junji;MUROMACHI Naoto;AKIYAMA Tomoki;Kim Jun-Dal;USUI Joichi;YAMAGATA Kunihiro;and FUKAMIZU Akiyoshi
  • 通讯作者:
    and FUKAMIZU Akiyoshi

NOGUCHI Kazuyuki的其他文献

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{{ truncateString('NOGUCHI Kazuyuki', 18)}}的其他基金

Development of novel periodontal/bone regenerative therapy utilizing LIPUS, BMP9, and dedifferentiated fat cells
利用 LIPUS、BMP9 和去分化脂肪细胞开发新型牙周/骨再生疗法
  • 批准号:
    19K10169
  • 财政年份:
    2019
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of new regeneration therapy using strong osteoplasty protein BMP-9 and cells derived from adipose tissue
使用强骨形成蛋白 BMP-9 和脂肪组织来源的细胞开发新的再生疗法
  • 批准号:
    15H05036
  • 财政年份:
    2015
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of the dental implant with a periodontal tissue using stem cells
利用干细胞开发带有牙周组织的牙种植体
  • 批准号:
    23659922
  • 财政年份:
    2011
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
A comprehensive study on periodontal regeneration using induced pluripotent stem(iPS) cells
使用诱导多能干(iPS)细胞进行牙周再生的综合研究
  • 批准号:
    21390525
  • 财政年份:
    2009
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Regulation of cementoblast differenciation by transcriptional factors and periodontal regeneration
转录因子对成牙骨质细胞分化和牙周再生的调节
  • 批准号:
    19592381
  • 财政年份:
    2007
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Elucidation of roles and functions of cementoblasts in the destruction and regeneration of periodontal tissue
阐明成牙骨质细胞在牙周组织破坏和再生中的作用和功能
  • 批准号:
    15592185
  • 财政年份:
    2003
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Cytological and pharmacological studies on the pathogenesis of nifedipine-associated gingival hyperplasia
硝苯地平相关牙龈增生发病机制的细胞学和药理学研究
  • 批准号:
    09671945
  • 财政年份:
    1997
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The roles of cyclooxygenase-2 in the pathogenesis of periodontal disease
环氧合酶2在牙周病发病机制中的作用
  • 批准号:
    07672073
  • 财政年份:
    1995
  • 资助金额:
    $ 2.5万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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Elucidation of CEBP beta expression control mechanism in hypoxia-induced modification human periodontal ligament cells
阐明缺氧诱导的修饰人牙周膜细胞CEBPβ表达控制机制
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牙周膜细胞与成牙骨质细胞CD40-CD40L相互作用的功能
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    21K21062
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    2021
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Stromal cell-derived factor1 expression on periodontal ligament cells derived from Down syndrom
唐氏综合症牙周膜细胞上基质细胞衍生因子1的表达
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    20K10235
  • 财政年份:
    2020
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球体培养在牙周膜细胞频繁传代恶化中的应用及分子基础
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基于牙周膜细胞向干细胞转化的牙周组织再生评价及其机制的阐明
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Investigating the mechanism to regulate osteocytes by circadian rhythm that effected by the crosstalk with periodontal ligament cells
探讨牙周膜细胞的昼夜节律调节骨细胞的机制
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    19J11906
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缺氧诱导牙周膜细胞细胞因子级联修饰的分子机制
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预测牙周膜细胞同种异体移植具有优异再生潜力的细胞
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    17K17346
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Characterization of Treponema Denticola-Mediated Interactions With Periodontal Ligament Cells Leading to Persistent Host Cell/Tissue Destruction
齿垢密螺旋体介导的与牙周膜细胞相互作用导致宿主细胞/组织持续破坏的特征
  • 批准号:
    10454807
  • 财政年份:
    2017
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    $ 2.5万
  • 项目类别:
Characterization of Treponema Denticola-Mediated Interactions With Periodontal Ligament Cells Leading to Persistent Host Cell/Tissue Destruction
齿垢密螺旋体介导的与牙周膜细胞相互作用导致宿主细胞/组织持续破坏的特征
  • 批准号:
    9470510
  • 财政年份:
    2017
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    $ 2.5万
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