Development of the dental implant with a periodontal tissue using stem cells

利用干细胞开发带有牙周组织的牙种植体

• 批准号: 23659922
• 负责人: NOGUCHI Kazuyuki
• 金额: $ 2.33万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659922/
• 关键词: 歯周組織; エナメルマトリックスタンパク; 幹細胞; インプラント; 歯周組織再生

Enamel matrix derivative (EMD) plays an important role in periodontal tissue regeneration by inducing angiogenesis. The present study was aimed to evaluate the kinetics of human mesenchymal stem cells (hMSC) or human periodontal ligament fibroblasts (hPDLs) on the titanium surface with biochemical treatment, namely, with or without EMD immobilization. EMD could be easily immobilized by tresyl chloride-activation technique. In the both hMSCs and hPDLs, the production of VEGF was significantly increased on titanium surface with EMD immobilization than other control groups. Furthermore, the mRNA levels ofPeriostinand PLAL-1, which were the markers of a periodontal ligament, were up-regulated in both hMSCs and hPDLs on titanium surface with EMD immobilization than other control groups. Further refinement of this approach will facilitate the development of clinically relevant methods for PDL regeneration on titanium implants in humans.

牙釉质基质衍生物（enamelmatrixderivatives，EMD）通过诱导血管生成在牙周组织再生中发挥重要作用.本研究的目的是评估人间充质干细胞（hMSC）或人牙周膜成纤维细胞（hPDL）在钛表面的生化处理，即，有或没有EMD固定的动力学。三甲苯基氯活化法可以很容易地固定EMD。在hMSCs和hPDLs中，EMD固定的钛表面上的VEGF的产生比其他对照组显著增加。此外，牙周膜标志物Periostin和PLAL-1的mRNA水平在EMD固定的钛表面上的hMSCs和hPDLs中均比其他对照组上调。这种方法的进一步完善将促进临床相关方法的发展，在人类钛种植体的PDL再生。

项目成果

Enamel matrix derivative induces VEGF production in human gingival fibroblasts

牙釉质基质衍生物诱导人牙龈成纤维细胞产生 VEGF

• 发表时间: 2012
• 作者: Sakoda K;Nakajima Y;Noguchi K.
• 通讯作者: Noguchi K.

Enamel matrix derivative induces production of vascular endothelial cell growth factor in human gingival fibroblasts

• DOI: 10.1111/j.1600-0722.2012.00999.x
• 发表时间: 2012-12-01
• 期刊: EUROPEAN JOURNAL OF ORAL SCIENCES
• 影响因子: 1.9
• 作者: Sakoda, Kenji;Nakajima, Yumiko;Noguchi, Kazuyuki
• 通讯作者: Noguchi, Kazuyuki