Development of the dental implant with a periodontal tissue using stem cells

釉质基质衍生物通过诱导血管生成，在牙周组织再生中发挥重要作用。本研究旨在评价人骨髓间充质干细胞(HMSC)或人牙周膜成纤维细胞(HPDL)在钛表面经生物化学处理后的动力学变化。甲苯氯活化法可以很容易地固定化EMD。在hMSCs和hPDL中，EMD固定组与其他对照组相比，钛表面血管内皮生长因子的产生均显著增加。此外，作为牙周韧带标志物的Periostin和PLAL-1在钛表面的hMSCs和hPDL中的表达水平在EMD固定组均高于其他对照组。这一方法的进一步完善将促进临床相关方法的发展，用于钛种植体上的PDL再生。

Enamel matrix derivative (EMD) plays an important role in periodontal tissue regeneration by inducing angiogenesis. The present study was aimed to evaluate the kinetics of human mesenchymal stem cells (hMSC) or human periodontal ligament fibroblasts (hPDLs) on the titanium surface with biochemical treatment, namely, with or without EMD immobilization. EMD could be easily immobilized by tresyl chloride-activation technique. In the both hMSCs and hPDLs, the production of VEGF was significantly increased on titanium surface with EMD immobilization than other control groups. Furthermore, the mRNA levels ofPeriostinand PLAL-1, which were the markers of a periodontal ligament, were up-regulated in both hMSCs and hPDLs on titanium surface with EMD immobilization than other control groups. Further refinement of this approach will facilitate the development of clinically relevant methods for PDL regeneration on titanium implants in humans.