A comprehensive study on periodontal regeneration using induced pluripotent stem(iPS) cells

本研究的目的是探讨诱导多能干细胞移植牙周再生的可能性，并建立人牙龈成纤维细胞衍生的诱导多能干细胞。在成骨培养基中培养iPS细胞，通过茜素红S染色和骨钙素阳性免疫染色观察细胞的钙配置。在大鼠牙周开孔模型中，移植诱导多能干细胞不仅能促进新骨的形成，还能促进脱落牙本质表面牙骨质的形成。成骨培养基刺激小鼠iPS细胞促进大鼠牙周开窗模型牙周再生。此外，我们建立了人牙龈成纤维细胞衍生的iPS细胞。这项研究的结果可能为新的牙周治疗奠定基础。

The purpose of this study was to investigate the possibility of the periodontal regeneration by iPS cell transplantation and to establish human gingival fibroblast derived iPS cell.Culturing iPS cells in osteogenic medium, we observed calcium diposition by Alizarin Red S staining and osteocalcin positive immunostaining cells. In rat periodontal fenestration model, transplantation of the iPS cells encouraged not only new bone formation but also cementum formation on the denuded dentin surface.Mouse iPS cells stimulated with osteogenic medium promote periodontal regeneration in rat periodontal fenestration model.Moreover, we established human gingival fibroblast derived iPS cell. The results of this study may underlie new periodontal therapy.