Detection of vancomycin-resistant MRSA in oral cavity of dental patients

牙科患者口腔中耐万古霉素MRSA的检测

• 批准号: 12672026
• 负责人: OKAHASHI Nobuo
• 金额: $ 2.05万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672026/
• 关键词: dental clinic; methicillin-resistance; Staphylococcus aureus; vancomycin; coagulase-negative staphylococci

Methicillin-resistant Staphylococcus aureus (MRSA) emerged in the 1980s as a major clinical and epidemiologic problem in hospitals. Very recently, emergence of vancomycin-resistance MRSA is reported around the world. In dental clinics, implantation is now popular method in order to treat patients with periodontal diseases. Such invasive treatments will increase the risk of infection by oral bacteria. In this project, the investigator study the distribution of vancomycin-resistant MRSA in oral cavity of dental patients.In the first year of this project, the investigator has established the PCR detection system for MRSA by using mecA, femA and IS431 probes. PCR analysis for mecA of isolated staphylococci will reveal whether the isolate is methicillin-resistant or not. Oral swab samples from healty dental patients were examined by using staphylococcus-selective Staphylococcus 110 agar plates. Most of them showed white colonies indicating that most of the isolates were coaglase-negative st … More aphylococci. Less than 1 % of the appeared colonies was the yellow-colony forming S. aureus. However, more than 7% of the coagulase-negative staphylococci were mecA-positive. These results suggested that whereas MRSA in oral cavity of healty human is negligible, a number of methicillin-resistant coaglase-negative staphylococci colonize in human oral cavity. Similar study was performed in oral cavity of physically handicapped dental patients. The percentage of mecA-positive staphylococci was significantly higher than that of healty dental patients. These results suggested that methicilin-resistant staphylococci colonize in oral cavity of handicapped persons. Taken together, the wide distribution of mecA-positive staphylococci may contribute the spread of antibiotics-resistance in many other oral and enteric bacteria.Since the investigator isolated less than 1 0 clones of MRSA from dental patients, no solid conclusion could obtained about the vancomycin-resistance. In these clones of MRSA, no vancomycin-resistant was found. Less

耐甲氧西林金黄色葡萄球菌（MRSA）出现于20世纪80年代，是医院的主要临床和流行病学问题。最近，世界各地报道了耐万古霉素MRSA的出现。在牙科诊所，种植牙是治疗牙周病的常用方法。这种侵入性治疗将增加口腔细菌感染的风险。在本项目中，研究者研究了万古霉素耐药MRSA在牙科患者口腔中的分布。在项目第一年，研究者使用mecA、femA和IS431探针建立了MRSA的PCR检测系统。对分离的葡萄球菌的mecA进行PCR分析，可以揭示该葡萄球菌是否耐甲氧西林。采用葡萄球菌选择性葡萄球菌110琼脂板对健康牙科患者的口腔拭子样本进行检测。多数菌落呈白色，表明大部分分离株为凝固酶阴性。出现的菌落中只有不到1%是形成金黄色葡萄球菌的黄色菌落。然而，超过7%的凝固酶阴性葡萄球菌为meca阳性。这些结果表明，尽管MRSA在健康人的口腔中可以忽略不计，但大量耐甲氧西林的凝固酶阴性葡萄球菌在人的口腔中定植。对残障牙科患者的口腔进行了类似的研究。meca阳性葡萄球菌比例明显高于健康口腔患者。提示耐甲氧西林葡萄球菌在残疾人口腔中有定植。综上所述，meca阳性葡萄球菌的广泛分布可能导致许多其他口腔和肠道细菌抗生素耐药性的传播。由于研究者从牙科患者中分离到的MRSA克隆不到10个，因此无法得出万古霉素耐药的可靠结论。在这些MRSA克隆中，没有发现万古霉素耐药性。少

项目成果

Yamato K, et al: "Dissociation of bone morphogenetic protein-mediated growth arrest and apoptosis of mouse B cells by HPV-16 E6/E7"Exp.cell Res.. 257. 198-205 (2000)

Yamato K 等人：“HPV-16 E6/E7 引起的骨形态发生蛋白介导的小鼠 B 细胞生长停滞和细胞凋亡的解离”Exp.cell Res. 257. 198-205 (2000)

Okahashi, N. et al.: "Osteoclast differentiation is associated with transient upregulation of cyclin-dependent kinase inhibitors p21(WAF1/ClP1)and p27(KlP1)"J. Cell. Biochem. 80. 339-345 (2001)

Okahashi, N. 等人：“破骨细胞分化与细胞周期蛋白依赖性激酶抑制剂 p21(WAF1/ClP1) 和 p27(KlP1) 的瞬时上调有关”J.

Murase, Y. et al.: "Possible involvement of protein kinases and Smad2 signaling pathways on ostecolast differentiation enhanced by activin A"J. Cell. Physiol.. 188. 236-242 (2001)

Murase, Y. 等人：“蛋白激酶和 Smad2 信号通路可能参与激活素 A 增强的成骨细胞分化”J。

Nonaka, K., et al.: "Involvement of caspases in apoptotic cell death of murine macrophages infected with Actinobacillus actinomycetemcomitans"Journal of Periodontal Research. 36. 40-47 (2001)

Nonaka, K.等人：“半胱天冬酶参与感染伴放线放线杆菌的小鼠巨噬细胞的凋亡细胞死亡”牙周研究杂志。

Okahashi,N. et al.: "Osteoclast differentiation is associated with transient upregulation of cyclin-dependent kinase inhibitors p21^<WAF1/CIP1> and p27^<KIP1>"J.Cell.Biochem.. 80. 339-345 (2001)

冈桥，N.